BMRB Entry 16885

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PDB ID: 2kwv
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16885
MolProbity Validation Chart

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Title:
Solution Structure of UBM1 of murine Polymerase iota in Complex with Ubiquitin
Deposition date:
2010-04-20
Original release date:
2010-10-14
Authors:
Burschowsky, Daniel; Rudolf, Fabian; Rabut, Gwenael; Herrmann, Torsten; Peter, Matthias; Wider, Gerhard
Citation:

Citation: Burschowsky, Daniel; Rudolf, Fabian; Rabut, Gwenael; Herrmann, Torsten; Matthias, Peter; Wider, Gerhard. "Structural Analysis of the Conserved Ubiquitin-binding Motifs (UBMs) of the Translesion Polymerase iota in Complex with Ubiquitin."  J. Biol. Chem. 286, 1364-1373 (2011).
PubMed: 20929865

Assembly members:

Assembly members:
DNA_polymerase_iota_UBM1, polymer, 48 residues, 5125.6 Da.
Ubiquitin, polymer, 76 residues, 8576.914 Da.

Natural source:

Natural source:   Common Name: House mouse   Taxonomy ID: 10013   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGEX-4T-2

Entity Sequences (FASTA):

Entity Sequences (FASTA):
DNA_polymerase_iota_UBM1: GSDTSDLPLQALPEGVDQEV FKQLPADIQEEILSGKSREN LKGKGSLS
Ubiquitin: MQIFVKTLTGKTITLEVEPS DTIENVKAKIQDKEGIPPDQ QRLIFAGKQLEDGRTLSDYN IQKESTLHLVLRLRGG

Data sets:
Data typeCount
13C chemical shifts423
15N chemical shifts131
1H chemical shifts909

Additional metadata:

  • Assembly
  • Samples and Experiments
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Assembly:

Entity Assembly IDEntity NameEntity ID
1UBM11
2Ubiquitin2

Entities:

Entity 1, UBM1 48 residues - 5125.6 Da.

Residues 485-486 represent non-native cloning artifacts. Residues 487-490 and 527-532 represent flexible flanking regions that are not shown in the Structure.

1   GLYSERASPTHRSERASPLEUPROLEUGLN
2   ALALEUPROGLUGLYVALASPGLNGLUVAL
3   PHELYSGLNLEUPROALAASPILEGLNGLU
4   GLUILELEUSERGLYLYSSERARGGLUASN
5   LEULYSGLYLYSGLYSERLEUSER

Entity 2, Ubiquitin 76 residues - 8576.914 Da.

1   METGLNILEPHEVALLYSTHRLEUTHRGLY
2   LYSTHRILETHRLEUGLUVALGLUPROSER
3   ASPTHRILEGLUASNVALLYSALALYSILE
4   GLNASPLYSGLUGLYILEPROPROASPGLN
5   GLNARGLEUILEPHEALAGLYLYSGLNLEU
6   GLUASPGLYARGTHRLEUSERASPTYRASN
7   ILEGLNLYSGLUSERTHRLEUHISLEUVAL
8   LEUARGLEUARGGLYGLY

Related Database Links:

PDB 2KWV 1AAR 1CMX 1D3Z 1F9J 1FXT 1G6J 1GJZ 1NBF 1OGW 1P3Q 1Q5W 1S1Q 1TBE 1UBI 1UBQ 1UZX 1V80 1V81 1VX7 1WR6 1WRD 1XD3 1XQQ 1YD8 1YIW 1YJ1 1YX5 1YX6 1ZGU 2AYO 2BGF 2C7M 2C7N 2D3G 2DEN 2DX5 2FCM 2FCN 2FCQ 2FCS 2FID 2FIF 2FUH 2G45 2GMI 2HD5 2HTH 2IBI 2J7Q 2JF5 2JRI 2JY6 2JZZ 2K25 2K39 2K6D 2K8B 2K8C 2KDE 2KDF 2KHW 2KJH 2KLG 2KN5 2KOX 2KTF 2KWU 2KX0 2L0F 2L0T 2L3Z 2LD9 2LJ5 2LVO 2LVP 2LVQ 2LZ6 2MBB 2MBH 2MBO 2MBQ 2MCN 2MJ5 2MJB 2MOR 2MRE 2MUR 2NR2 2O6V 2OJR 2OOB 2PE9 2PEA 2QHO 2RR9 2RSU 2RU6 2W9N 2WDT 2WWZ 2WX0 2WX1 2XBB 2XEW 2XK5 2Y5B 2Z59 2ZCB 2ZCC 2ZNV 2ZVN 2ZVO 3A1Q 3A33 3A9J 3A9K 3AI5 3ALB 3AUL 3AXC 3B08 3B0A 3BY4 3C0R 3DVG 3DVN 3EEC 3EFU 3EHV 3H1U 3H7P 3H7S 3HM3 3I3T 3IFW 3IHP 3JSV 3JVZ 3JW0 3K9O 3K9P 3KVF 3KW5 3LDZ 3M3J 3MHS 3MTN 3N30 3N32 3NHE 3NOB 3NS8 3O65 3OFI 3OJ3 3OJ4 3ONS 3PHD 3PHW 3PRM 3PRP 3PT2 3PTF 3Q3F 3RUL 3TBL 3TMP 3U30 3UGB 3VDZ 3VFK 3VHT 3VUW 3VUX 3VUY 3ZLZ 3ZNH 3ZNI 3ZNZ 4A18 4A19 4A1B 4A1D 4ADX 4AP4 4AUQ 4BBN 4BOS 4BOZ 4BVU 4CXC 4CXD 4DDG 4DDI 4DHJ 4DHZ 4FJV 4HXD 4I6N 4IG7 4IUM 4JIO 4JQW 4K1R 4K7S 4K7U 4K7W 4KSK 4KSL 4KZX 4KZY 4KZZ 4LCD 4LDT 4LJO 4LJP 4M0W 4MDK 4MM3 4MSM 4MSQ 4NQK 4NQL 4P4H 4PIG 4PIH 4PIJ 4PQT 4RF0 4RF1 4UN2 4UPX 4UQ1 4UQ4 4UQ5 4W20 4W22 4W23 4W25 4W27 4W28 4WUR 4WZP
DBJ BAC25154 BAE26322 BAE32662 BAA03983 BAA09860 BAA11842 BAA11843 BAA23486
GB AAD50424 AAH82278 AAS75834 AAS75835 AEQ34091 AAA02769 AAA28154 AAA28997 AAA28998 AAA28999
REF NP_001129562 NP_001276444 NP_001276445 NP_036102 XP_006526008 NP_001005123 NP_001006688 NP_001009117 NP_001009202 NP_001009286
SP Q6R3M4 P0C273 P0C275 P0C276 P0CG47 P0CG48
BMRB 11505 11547 15047 15410 15689 15866 15907 16228 16582 16626 16763 16880 16895 17059 17181 17239 17333 17439 17769 17919 18582 18583 18584 18610 18611 18737 19394 19399 19406 19412 19447 25070 25230 4245 4375 4983 5101 5387 6457 6466 6470 6488 68 7111
EMBL CAA25706 CAA26488 CAA28495 CAA30183 CAA30815
PIR I50437 I51568 I65237 JN0790 S13928
PRF 0412265A 1101405A 1212243A 1212243B 1212243C
TPD FAA00319
TPG DAA18802 DAA20663 DAA20672 DAA24675 DAA28295
AlphaFold Q6R3M4 P0C273 P0C275 P0C276 P0CG47 P0CG48

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