BMRB Entry 4983
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4983
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Citation: Rajesh, Sundaresan. "Backbone 1H,13C,15N and side chain 13C assignment of YUH1-Ub in a 35 kDa
covalent complex" J. Biomol. NMR ., .-..
Assembly members:
Yuh1, polymer, 236 residues, Formula weight is not available
Ubiquitin, polymer, 76 residues, Formula weight is not available
Natural source: Common Name: Baker's yeast Taxonomy ID: 4932 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Saccharomyces cerevisiae
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
Yuh1: MSGENRAVVPIESNPEVFTN
FAHKLGLKNEWAYFDIYSLT
EPELLAFLPRPVKAIVLLFP
INEDRKSSTSQQITSSYDVI
WFKQSVKNACGLYAILHSLS
NNQSLLEPGSDLDNFLKSQS
DTSSSKNRFDDVTTDQFVLN
VIKENVQTFSTGQSEAPEAT
ADTNLHYITYVEENGGIFEL
DGRNLSGPLYLGKSDPTATD
LIEQELVRVRVASYMENANE
EDVLNFAMLGLGPNWE
Ubiquitin: MQIFVKTLTGKTITLEVEPS
DTIDNVKSKIQDKEGIPPDQ
QRLIFAGKQLEDGRTLSDYN
IQKESTLHLVLRLRGX
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 293 |
| 15N chemical shifts | 293 |
| 13C chemical shifts | 1182 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | YUH1 monomer | 1 |
| 2 | Ubiquitin | 2 |
Entities:
Entity 1, YUH1 monomer 236 residues - Formula weight is not available
| 1 | MET | SER | GLY | GLU | ASN | ARG | ALA | VAL | VAL | PRO | ||||
| 2 | ILE | GLU | SER | ASN | PRO | GLU | VAL | PHE | THR | ASN | ||||
| 3 | PHE | ALA | HIS | LYS | LEU | GLY | LEU | LYS | ASN | GLU | ||||
| 4 | TRP | ALA | TYR | PHE | ASP | ILE | TYR | SER | LEU | THR | ||||
| 5 | GLU | PRO | GLU | LEU | LEU | ALA | PHE | LEU | PRO | ARG | ||||
| 6 | PRO | VAL | LYS | ALA | ILE | VAL | LEU | LEU | PHE | PRO | ||||
| 7 | ILE | ASN | GLU | ASP | ARG | LYS | SER | SER | THR | SER | ||||
| 8 | GLN | GLN | ILE | THR | SER | SER | TYR | ASP | VAL | ILE | ||||
| 9 | TRP | PHE | LYS | GLN | SER | VAL | LYS | ASN | ALA | CYS | ||||
| 10 | GLY | LEU | TYR | ALA | ILE | LEU | HIS | SER | LEU | SER | ||||
| 11 | ASN | ASN | GLN | SER | LEU | LEU | GLU | PRO | GLY | SER | ||||
| 12 | ASP | LEU | ASP | ASN | PHE | LEU | LYS | SER | GLN | SER | ||||
| 13 | ASP | THR | SER | SER | SER | LYS | ASN | ARG | PHE | ASP | ||||
| 14 | ASP | VAL | THR | THR | ASP | GLN | PHE | VAL | LEU | ASN | ||||
| 15 | VAL | ILE | LYS | GLU | ASN | VAL | GLN | THR | PHE | SER | ||||
| 16 | THR | GLY | GLN | SER | GLU | ALA | PRO | GLU | ALA | THR | ||||
| 17 | ALA | ASP | THR | ASN | LEU | HIS | TYR | ILE | THR | TYR | ||||
| 18 | VAL | GLU | GLU | ASN | GLY | GLY | ILE | PHE | GLU | LEU | ||||
| 19 | ASP | GLY | ARG | ASN | LEU | SER | GLY | PRO | LEU | TYR | ||||
| 20 | LEU | GLY | LYS | SER | ASP | PRO | THR | ALA | THR | ASP | ||||
| 21 | LEU | ILE | GLU | GLN | GLU | LEU | VAL | ARG | VAL | ARG | ||||
| 22 | VAL | ALA | SER | TYR | MET | GLU | ASN | ALA | ASN | GLU | ||||
| 23 | GLU | ASP | VAL | LEU | ASN | PHE | ALA | MET | LEU | GLY | ||||
| 24 | LEU | GLY | PRO | ASN | TRP | GLU |
Entity 2, Ubiquitin 76 residues - Formula weight is not available
| 1 | MET | GLN | ILE | PHE | VAL | LYS | THR | LEU | THR | GLY | ||||
| 2 | LYS | THR | ILE | THR | LEU | GLU | VAL | GLU | PRO | SER | ||||
| 3 | ASP | THR | ILE | ASP | ASN | VAL | LYS | SER | LYS | ILE | ||||
| 4 | GLN | ASP | LYS | GLU | GLY | ILE | PRO | PRO | ASP | GLN | ||||
| 5 | GLN | ARG | LEU | ILE | PHE | ALA | GLY | LYS | GLN | LEU | ||||
| 6 | GLU | ASP | GLY | ARG | THR | LEU | SER | ASP | TYR | ASN | ||||
| 7 | ILE | GLN | LYS | GLU | SER | THR | LEU | HIS | LEU | VAL | ||||
| 8 | LEU | ARG | LEU | ARG | GLY | GLZ |
Samples:
sample_1: Yuh1, [U-95% 2H; U-13C; U-15N], 2 mM; Ubiquitin, [U-95% 2H; U-13C; U-15N], 2 mM
Cond_1: pH: 6.0; temperature: 303 K; ionic strength: 0.10 M
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H-15N HSQC | sample_1 | not available | Cond_1 |
| HNCO | sample_1 | not available | Cond_1 |
| HN(CA)CO | sample_1 | not available | Cond_1 |
| HNCA | sample_1 | not available | Cond_1 |
| HN(CO)CA | sample_1 | not available | Cond_1 |
| HNCACB | sample_1 | not available | Cond_1 |
| HN(COCA)CB | sample_1 | not available | Cond_1 |
| CCNNH | sample_1 | not available | Cond_1 |
| CC(CO)NNH | sample_1 | not available | Cond_1 |
Software:
No software information available
NMR spectrometers:
- Bruker DRX 600 MHz
Related Database Links:
| BMRB | 15047 15410 15592 15866 15907 16582 16626 16763 16880 16885 17024 17059 17181 17239 17333 17439 17769 17919 18582 18583 18584 18610 18611 18737 19394 19399 19406 19412 19447 25070 25230 4245 4375 4769 5387 6457 6466 6470 6488 68 7111 |
| PDB | |
| DBJ | BAA03983 BAA11842 BAA11843 BAA23632 BAA76889 |
| EMBL | CAA07773 CAA11267 CAA21278 CAA28495 CAA29195 CAK44245.1 CAB90826.1 CAB90826.1 CAI77901.1 |
| GB | AAA03351 AAA28997 AAA28998 AAA28999 AAA29001 |
| PIR | I50437 I51568 I65237 S13928 S21083 |
| PRF | 1212243A 1212243C 1212243J 1604470A 1911411A |
| REF | NP_001005123 NP_001006688 NP_001009117 NP_001009286 NP_001013290 XP_001643983.1 XP_001643983.1 XP_001643983.1 XP_001643983.1 XP_001645736.1 |
| SP | P05759 P0C016 P0C224 P0C273 P0C275 |
| TPD | FAA00316 FAA00317 FAA00318 FAA00319 FAA00318.1 FAA00316.1 FAA00316.1 FAA00316.1 FAA00316.1 |
| TPE | CBF76581 CBF85986 |
| TPG | DAA08405 DAA09244 DAA09283 DAA09489 DAA18802 |
| GenBank | AAD44041.1 AAA36787.1 ABC94632.1 EDM13556.1 EDO17878.1 |
| AlphaFold | P05759 P0C016 P0C224 P0C273 P0C275 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks