BMRB Entry 25230
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PDB ID: 2mur
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR25230
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
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Citation: Wojtaszek, Jessica; Wang, Su; Kim, Hyungjin; Wu, Qinglin; D'Andrea, Alan; Zhou, Pei. "Ubiquitin recognition by FAAP20 expands the complex interface beyond the canonical UBZ domain" Nucleic Acids Res. 42, 13997-14005 (2014).
PubMed: 25414354
Assembly members:
UBZ, polymer, 44 residues, 4853.517 Da.
Ubiquitin, polymer, 78 residues, 8802.141 Da.
entity_ZN, non-polymer, 65.409 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pet15b
Entity Sequences (FASTA):
UBZ: SHMGAAALRSCPMCQKEFAP
RLTQLDVDSHLAQCLAESTE
DVTW
Ubiquitin: SHMQIFVKTLTGKTITLEVE
PSDTIENVKAKIQDKEGIPP
DQQRLIFAGKQLEDGRTLSD
YNIQKESTLHLVLRLRGG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 522 |
| 15N chemical shifts | 122 |
| 1H chemical shifts | 860 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | UBZ | 1 |
| 2 | Ubiquitin | 2 |
| 3 | ZINC ION | 3 |
Entities:
Entity 1, UBZ 44 residues - 4853.517 Da.
Residues 1-3 represent non-native residues leftover after protease cleavage removal of a affinity tag; Residues 4-44 represent the UBZ domain of FAAP20(140-180).
| 1 | SER | HIS | MET | GLY | ALA | ALA | ALA | LEU | ARG | SER | ||||
| 2 | CYS | PRO | MET | CYS | GLN | LYS | GLU | PHE | ALA | PRO | ||||
| 3 | ARG | LEU | THR | GLN | LEU | ASP | VAL | ASP | SER | HIS | ||||
| 4 | LEU | ALA | GLN | CYS | LEU | ALA | GLU | SER | THR | GLU | ||||
| 5 | ASP | VAL | THR | TRP |
Entity 2, Ubiquitin 78 residues - 8802.141 Da.
Residues 1-2 represent non-native residues leftover after protease cleavage removal of an affinity tag; residues 3-78 represent human ubiquitin sequence (1-76).
| 1 | SER | HIS | MET | GLN | ILE | PHE | VAL | LYS | THR | LEU | ||||
| 2 | THR | GLY | LYS | THR | ILE | THR | LEU | GLU | VAL | GLU | ||||
| 3 | PRO | SER | ASP | THR | ILE | GLU | ASN | VAL | LYS | ALA | ||||
| 4 | LYS | ILE | GLN | ASP | LYS | GLU | GLY | ILE | PRO | PRO | ||||
| 5 | ASP | GLN | GLN | ARG | LEU | ILE | PHE | ALA | GLY | LYS | ||||
| 6 | GLN | LEU | GLU | ASP | GLY | ARG | THR | LEU | SER | ASP | ||||
| 7 | TYR | ASN | ILE | GLN | LYS | GLU | SER | THR | LEU | HIS | ||||
| 8 | LEU | VAL | LEU | ARG | LEU | ARG | GLY | GLY |
Entity 3, ZINC ION - Zn - 65.409 Da.
| 1 | ZN |
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