BMRB Entry 5387

Title:
Complete 1H, 13C, and 15N chemical shifts for human recombinant ubiquitin in reverse micelles
Deposition date:
2002-06-04
Original release date:
2003-06-12
Authors:
Babu, Charles; Flynn, Peter; Wand, A. Joshua
Citation:

Citation: Babu, Charles; Flynn, Peter; Wand, A.. "Validation of protein structure from preparations of encapsulated proteins dissolved in low viscosity fluids"  J. Am. Chem. Soc. 123, 2691-2692 (2001).
PubMed: 11456950

Assembly members:

Assembly members:
ubiquitin, polymer, 76 residues, 8565 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET

Entity Sequences (FASTA):

Data sets:
Data typeCount
1H chemical shifts576
13C chemical shifts340
15N chemical shifts82

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1ubiquitin1

Entities:

Entity 1, ubiquitin 76 residues - 8565 Da.

1   METGLNILEPHEVALLYSTHRLEUTHRGLY
2   LYSTHRILETHRLEUGLUVALGLUPROSER
3   ASPTHRILEGLUASNVALLYSALALYSILE
4   GLNASPLYSGLUGLYILEPROPROASPGLN
5   GLNARGLEUILEPHEALAGLYLYSGLNLEU
6   GLUASPGLYARGTHRLEUSERASPTYRASN
7   ILEGLNLYSGLUSERTHRLEUHISLEUVAL
8   LEUARGLEUARGGLYGLY

Samples:

sample_1: ubiquitin, [U-13C; U-15N], 0.3 mM; d-pentane 98%

Ex-cond_1: pH: 5.0 na; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
HNCOnot availablenot availablenot available
HNCACBnot availablenot availablenot available
CBCA(CO)NHnot availablenot availablenot available
CC(CO)NHnot availablenot availablenot available
TOCSY-15N-HSQCnot availablenot availablenot available
HCCH-TOCSYnot availablenot availablenot available
CT-13C-HSQCnot availablenot availablenot available
15N-HSQCnot availablenot availablenot available

Software:

No software information available

NMR spectrometers:

  • Varian INOVA 600 MHz
  • Varian INOVA 750 MHz

Related Database Links:

BMRB 11505 11547 15047 15410 15689 15866 15907 16228 16582 16626 16763 16880 16885 16895 17059 17181 17239 17333 17439 17769 17919 18582 18583 18584 18610 18611 18737 19394 19399 19406 19412 19447 25070 25230 4245 4375 4983 5101 6457 6466 6470 6488 68 7111
PDB
DBJ BAA03983 BAA09860 BAA11842 BAA11843 BAA23486
EMBL CAA25706 CAA26488 CAA28495 CAA30183 CAA30815
GB AAA02769 AAA28154 AAA28997 AAA28998 AAA28999
PIR I50437 I51568 I65237 JN0790 S13928
PRF 0412265A 1101405A 1212243A 1212243B 1212243C
REF NP_001005123 NP_001006688 NP_001009117 NP_001009202 NP_001009286
SP P0C273 P0C275 P0C276 P0CG47 P0CG48
TPD FAA00319
TPG DAA18802 DAA20663 DAA20672 DAA24675 DAA28295
AlphaFold P0C273 P0C275 P0C276 P0CG47 P0CG48 P0C273 P0C275 P0C276 P0CG47 P0CG48 P0C273 P0C275 P0C276 P0CG47 P0CG48

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks