BMRB Entry 5101

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5101
MolProbity Validation Chart

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Title:
Structure and Properties of a Dimeric N-terminal Fragment of Human Ubiquitin
Deposition date:
2001-08-06
Original release date:
2002-01-25
Authors:
Bolton, David; Evans, Philip; Stott, Katherine; Broadhurst, Richard
Citation:

Citation: Bolton, David; Evans, Philip; Stott, Katherine; Broadhurst, Richard. "Structure and Properties of a Dimeric N-terminal Fragment of Human Ubiquitin"  J. Mol. Biol. 314, 773-787 (2001).
PubMed: 117733996

Assembly members:

Assembly members:
Ubiquitin, polymer, 53 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGEX-4T3

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Ubiquitin: GSMQIFVKTLTGKTITLEVE PSDTIENVKAKIQDKEGIPP DQQRLIFAGKQLE

Data sets:
Data typeCount
1H chemical shifts390
13C chemical shifts174
15N chemical shifts49

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1uq1_51 subunit A1
2uq1_51 subunit B1

Entities:

Entity 1, uq1_51 subunit A 53 residues - Formula weight is not available

1   GLYSERMETGLNILEPHEVALLYSTHRLEU
2   THRGLYLYSTHRILETHRLEUGLUVALGLU
3   PROSERASPTHRILEGLUASNVALLYSALA
4   LYSILEGLNASPLYSGLUGLYILEPROPRO
5   ASPGLNGLNARGLEUILEPHEALAGLYLYS
6   GLNLEUGLU

Samples:

sample_1: Ubiquitin, [U-100% 13C; U-100% 15N], 1.0 mM; sodium sulphate 0.8 M

sample_2: Ubiquitin 1.0 mM; sodium sulphate 0.8 M

conditions_1: pH: 7.0 na; temperature: 298 K; ionic strength: 0.8 M; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
3D 1H-1H-15N NOESYnot availablenot availablenot available
3D 1H-1H-15N TOCSYnot availablenot availablenot available
3D 13C-1H-1H NOESYnot availablenot availablenot available
3D 1H-13C-1H HCCH-TOCSYnot availablenot availablenot available
3D CBCA(CO)NHnot availablenot availablenot available
3D HNCAnot availablenot availablenot available
3D HN(CO)CAnot availablenot availablenot available

Software:

AZARA - data processing

ANSIG v3.3 - assignment

NMR spectrometers:

  • Bruker DRX 500 MHz

Related Database Links:

BMRB 11505 11547 15047 15410 15689 15866 15907 16228 16582 16626 16763 16880 16885 16895 17059 17181 17239 17333 17439 17769 17919 18582 18583 18584 18610 18611 18737 19394 19399 19406 19412 19447 25070 25230 4245 4375 5387 6457 6466 6470 6488 68 7111
PDB
DBJ BAA03983 BAA11389 BAA11842 BAA11843 BAA23486
EMBL CAA28495 CAA30815 CAA33466 CAA35999 CAA37227
GB AAA02769 AAA28153 AAA28154 AAA28997 AAA28998
PIR I50437 I51568 I65237 JN0790 S13928
PRF 0412265A 1212243A 1212243B 1212243C 1212243D
REF NP_001005123 NP_001006688 NP_001009117 NP_001009202 NP_001009286
SP P0C273 P0C275 P0C276 P0CG47 P0CG48
TPG DAA18802 DAA20663 DAA20672 DAA24675 DAA26453
AlphaFold P0C273 P0C275 P0C276 P0CG47 P0CG48

Download HSQC peak lists in one of the following formats:
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SPARKY: Backbone or all simulated peaks