BMRB Entry 16215

Name: Refined solution structure of des-pyro Glu brazzein
Published: 2013-01-28

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PDB ID: 2ly5
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16215
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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Refined solution structure of des-pyro Glu brazzein

Deposition date:

2009-03-13

Original release date:

2013-01-28

Authors:

Cornilescu, Claudia; Tonelli, Marco; DeRider, Michele; Markley, John; Assadi-Porter, Fariba

Citation:

Citation: Cornilescu, Claudia; Cornilescu, Gabriel; Rao, Hongyu; Porter, Sarah; Tonelli, Marco; DeRider, Michele; Markley, John; Assadi-Porter, Fariba. "Temperature-dependent conformational change affecting Tyr11 and sweetness loops of brazzein." Proteins 81, 919-925 (2013).
PubMed: 23349025

Assembly members:

Assembly members:
wt-brazzein, polymer, 53 residues, 6523.365 Da.

Natural source:

Natural source: Common Name: Pentadiplandra brazzeana Taxonomy ID: 43545 Superkingdom: Eukaryota Kingdom: Viridiplantae Genus/species: Pentadiplandra brazzeana

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET3a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
wt-brazzein: DKCKKVYENYPVSKCQLANQ CNYDCKLDKHARSGECFYDE KRNLQCICDYCEY

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	221
15N chemical shifts	59
1H chemical shifts	349

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	wt-brazzein	1

Entities:

Entity 1, wt-brazzein 53 residues - 6523.365 Da.

1

ASP

LYS

CYS

LYS

VAL

TYR

GLU

ASN

TYR

2

PRO

VAL

SER

LYS

CYS

GLN

LEU

ALA

ASN

GLN

3

CYS

ASN

TYR

ASP

CYS

LYS

LEU

ASP

LYS

HIS

4

ALA

ARG

SER

GLY

GLU

CYS

PHE

TYR

ASP

GLU

5

LYS

ARG

ASN

LEU

GLN

CYS

ILE

CYS

ASP

TYR

6

CYS

GLU

TYR

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	cs_sample	isotropic	conditions4cs
2D 1H-13C HSQC	cs_sample	isotropic	conditions4cs
3D HNCO	cs_sample	isotropic	conditions4cs
3D HNCA	cs_sample	isotropic	conditions4cs
3D HNCACB	cs_sample	isotropic	conditions4cs
3D CBCA(CO)NH	cs_sample	isotropic	conditions4cs
3D HN(CO)CA	cs_sample	isotropic	conditions4cs
3D HBHA(CO)NH	cs_sample	isotropic	conditions4cs
3D HCACO	cs_sample	isotropic	conditions4cs
3D HCCH-COSY	cs_sample	isotropic	conditions4cs
3D 1H-15N TOCSY	cs2_sample	isotropic	conditions4cs
3D 1H-15N NOESY	cs2_sample	isotropic	conditions4cs
3D 1H-13C NOESY	cs_sample	isotropic	conditions4cs
IPAP-HSQC	cs2_sample	isotropic	conditions4cs
IPAP-HSQC	rdc_sample	anisotropic	conditions4cs

BMRB	16978 18710 4067
PDB	1BRZ 2BRZ 2KGQ 2KYQ 2LY5 2LY6 4HE7
GB	ACK76425 AGM38242
SP	P56552
AlphaFold	P56552

BMRB Entry 16215

Additional metadata:

Assembly:

Entities:

Samples:

Experiments:

Software:

NMR spectrometers:

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