BMRB Entry 6956

Title:
RNA recognition by the Vts1 SAM domain   PubMed: 16429155
Deposition date:
2006-01-27
Original release date:
2006-04-26
Authors:
Donaldson, L.; Johnson, P.
Citation:

Citation: Johnson, P.; Donaldson, L.. "RNA recognition by the Vts1p SAM domain"  Nat. Struct. Mol. Biol. 13, 177-178 (2006).

Assembly members:

Assembly members:
Vts1p, polymer, 119 residues, Formula weight is not available
RNA, polymer, 19 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: baker's yeast   Taxonomy ID: 4932   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Saccharomyces cerevisiae

Experimental source:   Production method: recombinant technology

Experimental source:

Natural source:   Common Name: baker's yeast   Taxonomy ID: 4932   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Saccharomyces cerevisiae

Experimental source:   Production method: recombinant technology

Data sets:
Data typeCount
13C chemical shifts352
15N chemical shifts83
1H chemical shifts613

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Vts1p1
25'-R(*GP*GP*AP*GP*GP*CP*UP*CP*UP*GP*GP*CP*AP*GP*CP*UP*UP*UP*C)-3'2

Entities:

Entity 1, Vts1p 119 residues - Formula weight is not available

1   GLYSERASNVALPHEASNASNTHRILETHR
2   HISPROASNALAGLYPROTHRSERALATHR
3   SERTHRSERTHRSERSERASNGLYASNTHR
4   PROLEUSERSERASNSERSERMETASNPRO
5   LYSSERLEUTHRASPPROLYSLEULEULYS
6   ASNILEPROMETTRPLEULYSSERLEUARG
7   LEUHISLYSTYRSERASPALALEUSERGLY
8   THRPROTRPILEGLULEUILETYRLEUASP
9   ASPGLUTHRLEUGLULYSLYSGLYVALLEU
10   ALALEUGLYALAARGARGLYSLEULEULYS
11   ALAPHEGLYILEVALILEASPTYRLYSGLU
12   ARGASPLEUILEASPARGSERALATYR

Entity 2, 5'-R(*GP*GP*AP*GP*GP*CP*UP*CP*UP*GP*GP*CP*AP*GP*CP*UP*UP*UP*C)-3' 19 residues - Formula weight is not available

1   GGAGGCUCUG
2   GCAGCUUUC

Samples:

sample_1: Vts1p, [U-15N; U-13C], 0.2 – 0.8 mM; RNA0.2 – 0.8 mM; sodium phosphate buffer 20 mM; sodium chloride 150 mM; sodium azide 0.02%; H2O 90%; D2O 10%

sample_cond_1: ionic strength: 150 mM; pH: 7.8; pressure: 1 atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
3D 13C-separated NOESYsample_1not availablesample_cond_1
3D 15N-separated NOESYsample_1not availablesample_cond_1
2D NOESYsample_1not availablesample_cond_1
2D 12C-filtered 13C-edited NOESYsample_1not availablesample_cond_1

Software:

CYANA v2.1 - structure solution

X-PLOR NIH v2.11.0 - refinement, structure solution

NMR spectrometers:

  • Varian INOVA 500 MHz

Related Database Links:

BMRB 6922
PDB
DBJ GAA26663
EMBL CAA99688
GB AHY77628 EDN63679 EDV10919 EDZ69055 EEU07517
REF NP_015004
SP Q08831
TPG DAA11120
AlphaFold Q08831

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts