BMRB Entry 6922

Title:
Solution structure of the Vts1 SAM domain in the presence of RNA   PubMed: 16405996
Deposition date:
2005-12-12
Original release date:
2007-02-06
Authors:
Edwards, Thomas; Butterwick, Joel; Palmer, Arthur; Aggarwal, Aneel
Citation:

Citation: Edwards, Thomas; Butterwick, Joel; Zeng, Lei; Gupta, Yogesh; Wang, Xin; Wharton, Robin; Palmer, Arthur; Aggarwal, Aneel. "Solution Structure of the Vts1 SAM Domain in the Presence of RNA"  J. Mol. Biol. 356, 1065-1072 (2006).

Assembly members:

Assembly members:
Vts1 (438-523), polymer, 86 residues, Formula weight is not available
TCE, polymer, 13 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Baker's yeast   Taxonomy ID: 4932   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Saccharomyces cerevisiae

Experimental source:   Production method: recombinant technology

Experimental source:

Natural source:   Common Name: Baker's yeast   Taxonomy ID: 4932   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Saccharomyces cerevisiae

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts399
15N chemical shifts89
1H chemical shifts607
residual dipolar couplings46

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Vts11
2RNA 13nt2

Entities:

Entity 1, Vts1 86 residues - Formula weight is not available

1   SERASNSERSERMETASNPROLYSSERLEU
2   THRASPPROLYSLEULEULYSASNILEPRO
3   METTRPLEULYSSERLEUARGLEUHISLYS
4   TYRSERASPALALEUSERGLYTHRPROTRP
5   ILEGLULEUILETYRLEUASPASPGLUTHR
6   LEUGLULYSLYSGLYVALLEUALALEUGLY
7   ALAARGARGLYSLEULEULYSALAPHEGLY
8   ILEVALILEASPTYRLYSGLUARGASPLEU
9   ILEASPARGSERALATYR

Entity 2, RNA 13nt 13 residues - Formula weight is not available

1   GGCUCUGGCA
2   GCC

Samples:

sample_1: Vts1 (438-523), [U-13C; U-15N], 0.5 mM; TCE 0.5 mM

conditions_1: pH: 6.25; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
1H15N_HSQCsample_1not availableconditions_1
HNCAsample_1not availableconditions_1
HN(CO)CAsample_1not availableconditions_1
HNCOsample_1not availableconditions_1
HN(CA)COsample_1not availableconditions_1
HNCACBsample_1not availableconditions_1
HCCH-COSYsample_1not availableconditions_1
13C-NOESYsample_1not availableconditions_1
15N-NOESYsample_1not availableconditions_1
H(CCO)NHsample_1not availableconditions_1

Software:

No software information available

NMR spectrometers:

  • . . 500 MHz

Related Database Links:

BMRB 6956
PDB
DBJ GAA26663
EMBL CAA99688
GB AHY77628 EDN63679 EDV10919 EDZ69055 EEU07517
REF NP_015004
SP Q08831
TPG DAA11120
AlphaFold Q08831

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts