BMRB Entry 6922

Name: Solution structure of the Vts1 SAM domain in the presence of RNA
Published: 2007-02-06

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PDB ID: 2fe9
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6922
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of the Vts1 SAM domain in the presence of RNA

Deposition date:

2005-12-12

Original release date:

2007-02-06

Authors:

Edwards, Thomas; Butterwick, Joel; Palmer, Arthur; Aggarwal, Aneel

Citation:

Citation: Edwards, Thomas; Butterwick, Joel; Zeng, Lei; Gupta, Yogesh; Wang, Xin; Wharton, Robin; Palmer, Arthur; Aggarwal, Aneel. "Solution Structure of the Vts1 SAM Domain in the Presence of RNA" J. Mol. Biol. 356, 1065-1072 (2006).
PubMed: 16405996

Assembly members:

Assembly members:
Vts1 (438-523), polymer, 86 residues, Formula weight is not available
TCE, polymer, 13 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Baker's yeast Taxonomy ID: 4932 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Vts1 (438-523): SNSSMNPKSLTDPKLLKNIP MWLKSLRLHKYSDALSGTPW IELIYLDDETLEKKGVLALG ARRKLLKAFGIVIDYKERDL IDRSAY
TCE: GGCUCUGGCAGCC

Data sets:

assigned_chemical_shifts
- chem_shift_list_1
RDCs
- RDC_list_1

Data type	Count
13C chemical shifts	399
15N chemical shifts	89
1H chemical shifts	607
residual dipolar couplings	46

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Vts1	1
2	RNA 13nt	2

Entities:

Entity 1, Vts1 86 residues - Formula weight is not available

1

SER

ASN

SER

MET

ASN

PRO

LYS

SER

LEU

2

THR

ASP

PRO

LYS

LEU

LYS

ASN

ILE

PRO

3

MET

TRP

LEU

LYS

SER

LEU

ARG

LEU

HIS

LYS

4

TYR

SER

ASP

ALA

LEU

SER

GLY

THR

PRO

TRP

5

ILE

GLU

LEU

ILE

TYR

LEU

ASP

GLU

THR

6

LEU

GLU

LYS

GLY

VAL

LEU

ALA

LEU

GLY

7

ALA

ARG

LYS

LEU

LYS

ALA

PHE

GLY

8

ILE

VAL

ILE

ASP

TYR

LYS

GLU

ARG

ASP

LEU

9

ILE

ASP

ARG

SER

ALA

TYR

Entity 2, RNA 13nt 13 residues - Formula weight is not available

1

G

C

U

C

U

G

C

A

2

G

C

Samples:

sample_1: Vts1 (438-523), [U-13C; U-15N], 0.5 mM; TCE 0.5 mM

conditions_1: pH: 6.25; temperature: 303 K

Experiments:

Name	Sample	Sample state	Sample conditions
1H15N_HSQC	sample_1	not available	conditions_1
HNCA	sample_1	not available	conditions_1
HN(CO)CA	sample_1	not available	conditions_1
HNCO	sample_1	not available	conditions_1
HN(CA)CO	sample_1	not available	conditions_1
HNCACB	sample_1	not available	conditions_1
HCCH-COSY	sample_1	not available	conditions_1
13C-NOESY	sample_1	not available	conditions_1
15N-NOESY	sample_1	not available	conditions_1
H(CCO)NH	sample_1	not available	conditions_1

Software:

No software information available

NMR spectrometers:

. . 500 MHz

BMRB	6956
PDB	2B6G 2D3D 2F8K 2FE9
DBJ	GAA26663
EMBL	CAA99688
GB	AHY77628 EDN63679 EDV10919 EDZ69055 EEU07517
REF	NP_015004
SP	Q08831
TPG	DAA11120
AlphaFold	Q08831