BMRB Entry 6409

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6409

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone 1H, 13C, and 15N and 13CB Chemical Shift Assignments for Four N-terminal C2H2 Zinc Fingers (F1-4) of MTF-1

Deposition date:

2004-12-03

Original release date:

2005-11-14

Authors:

Laity, John; Potter, Belinda; Knudsen, Nathan

Citation:

Citation: Potter, Belinda; Feng, Linda; Parasuram, Priya; Matskevich, Viktor; Wilson, Jed; Andrews, Glen; Laity, John. "The six zinc fingers of metal-responsive element binding transcription factor-1 form stable and quasi-ordered structures with relatively small differences in zinc affinities." J. Biol. Chem. 280, 28529-28540 (2005).
PubMed: 16055450

Assembly members:

Assembly members:
metal-responsive element-binding transcription factor-1, polymer, 117 residues, 13652.5 Da.
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source: Common Name: mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus

Experimental source:

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
metal-responsive element-binding transcription factor-1: VKRYQCTFEGCPRTYSTAGN LRTHQKTHRGEYTFVCNQEG CGKAFLTSYSLRIHVRVHTK EKPFECDVQGCEKAFNTLYR LKAHQRLHTGKTFNCESQGC SKYFTTLSDLRKHIRTH

Data sets:

assigned_chemical_shifts
- MTF-1_F14

Data type	Count
1H chemical shifts	108
13C chemical shifts	321
15N chemical shifts	108

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	MTF-1 subunit 1	1
2	Zn (II) A	2
3	Zn (II) B	2
4	Zn (II) C	2
5	Zn (II) D	2

Entities:

Entity 1, MTF-1 subunit 1 117 residues - 13652.5 Da.

1

VAL

LYS

ARG

TYR

GLN

CYS

THR

PHE

GLU

GLY

2

CYS

PRO

ARG

THR

TYR

SER

THR

ALA

GLY

ASN

3

LEU

ARG

THR

HIS

GLN

LYS

THR

HIS

ARG

GLY

4

GLU

TYR

THR

PHE

VAL

CYS

ASN

GLN

GLU

GLY

5

CYS

GLY

LYS

ALA

PHE

LEU

THR

SER

TYR

SER

6

LEU

ARG

ILE

HIS

VAL

ARG

VAL

HIS

THR

LYS

7

GLU

LYS

PRO

PHE

GLU

CYS

ASP

VAL

GLN

GLY

8

CYS

GLU

LYS

ALA

PHE

ASN

THR

LEU

TYR

ARG

9

LEU

LYS

ALA

HIS

GLN

ARG

LEU

HIS

THR

GLY

10

LYS

THR

PHE

ASN

CYS

GLU

SER

GLN

GLY

CYS

11

SER

LYS

TYR

PHE

THR

LEU

SER

ASP

LEU

12

ARG

LYS

HIS

ILE

ARG

THR

HIS

Entity 2, Zn (II) A - Zn - 65.409 Da.

1

ZN

Samples:

all_samples: metal-responsive element-binding transcription factor-1, [U-98% 13C; U-98% 15N], 0.7 – 1.0 mM; B-mercaptoethanol 5 mM; MES 20 mM; H2O 95%; D2O 5%

cond_1: pH: 6.9; temperature: 293 K; ionic strength: 0.01 M

Experiments:

Name	Sample	Sample state	Sample conditions
1H-15N HSQC	all_samples	not available	cond_1
HNCACB	all_samples	not available	cond_1
CBCA(CO)NH	all_samples	not available	cond_1
HNCO	all_samples	not available	cond_1
HNCA	all_samples	not available	cond_1

Software:

NMRView v5.0.4 - Data analysis

NMRPipe vNMRPipe - Raw spectral data processing

NMR spectrometers:

Varian INOVA 600 MHz

SWISS-PROT	Q07243 Q14872
REF	XP_001503611.1 XP_513329.2 NP_032662.2 NP_001030252.2 NP_001102147.1
GenBank	EDL80405.1 AAH17679.1 EDL30350.1 AAP31813.1 EDL30349.1
EMBL	CAH70087.1 CAA55363.1 CAA50470.1 CAB71344.1
DBJ	BAE00336.1 BAC26879.1 BAA22262.1
BMRB	6445 6275 6276