BMRB Entry 6276

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6276

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Title:
1H, 13C and 15N resonance assignments for the six-zinc finger domain of the MTF-1 metalloregulatory protein in the DNA bound state
Deposition date:
2004-07-27
Original release date:
2005-07-25
Authors:
Feng, Linda; Knudsen, Nathan; Andrews, Glen; Laity, John; Potter, Belinda; Matskevich, Viktor
Citation:

Citation: Potter, Belinda; Knudsen, Nathan; Feng, Linda; Matskevich, Viktor; Wilson, Jed; Andrews, Glen; Laity, John. "NMR assignment of the six zinc fingers of MTF-1 in the free and DNA-bound states"  J. Biomol. NMR 31, 94-94 (2005).

Assembly members:

Assembly members:
Metal response element, polymer, 17 residues, Formula weight is not available
Metal response element, polymer, 17 residues, Formula weight is not available
metal-response element-binding transcription factor-1, polymer, 177 residues, 20367 Da.
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source:   Common Name: mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Metal response element: GCTCTGCACTCCGCCCG
Metal response element: CGGGCGGAGTGCAGAGC
metal-response element-binding transcription factor-1: VKRYQCTFEGCPRTYSTAGN LRTHQKTHRGEYTFVCNQEG CGKAFLTSYSLRIHVRVHTK EKPFECDVQGCEKAFNTLYR LKAHQRLHTGKTFNCESQGC SKYFTTLSDLRKHIRTHTGE KPFRCDHDGCGKAFAASHHL KTHVRTHTGERPFFCPSNGC EKTFSTQYSLKSHMKGH

Data sets:
Data typeCount
13C chemical shifts453
1H chemical shifts158
15N chemical shifts158

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1MTF-1 monomer3
2DNA strand one1
3DNA strand two2
4ZINC (II) ION 14
5ZINC (II) ION 24
6ZINC (II) ION 34
7ZINC (II) ION 44
8ZINC (II) ION 54
9ZINC (II) ION 64

Entities:

Entity 3, MTF-1 monomer 177 residues - 20367 Da.

1   VALLYSARGTYRGLNCYSTHRPHEGLUGLY
2   CYSPROARGTHRTYRSERTHRALAGLYASN
3   LEUARGTHRHISGLNLYSTHRHISARGGLY
4   GLUTYRTHRPHEVALCYSASNGLNGLUGLY
5   CYSGLYLYSALAPHELEUTHRSERTYRSER
6   LEUARGILEHISVALARGVALHISTHRLYS
7   GLULYSPROPHEGLUCYSASPVALGLNGLY
8   CYSGLULYSALAPHEASNTHRLEUTYRARG
9   LEULYSALAHISGLNARGLEUHISTHRGLY
10   LYSTHRPHEASNCYSGLUSERGLNGLYCYS
11   SERLYSTYRPHETHRTHRLEUSERASPLEU
12   ARGLYSHISILEARGTHRHISTHRGLYGLU
13   LYSPROPHEARGCYSASPHISASPGLYCYS
14   GLYLYSALAPHEALAALASERHISHISLEU
15   LYSTHRHISVALARGTHRHISTHRGLYGLU
16   ARGPROPHEPHECYSPROSERASNGLYCYS
17   GLULYSTHRPHESERTHRGLNTYRSERLEU
18   LYSSERHISMETLYSGLYHIS

Entity 1, DNA strand one 17 residues - Formula weight is not available

1   DGDCDTDCDTDGDCDADCDT
2   DCDCDGDCDCDCDG

Entity 2, DNA strand two 17 residues - Formula weight is not available

1   DCDGDGDGDCDGDGDADGDT
2   DGDCDADGDADGDC

Entity 4, ZINC (II) ION 1 - Zn - 65.409 Da.

1   ZN

Samples:

all_samples: metal-response element-binding transcription factor-1, [U-80% 2H; U-98% 13C; U-98% 15N], 0.4 mM; 2-Morpholinoethanesulfonic acid 20 mM

Cond_1: pH: 6.9; temperature: 303 K; ionic strength: 0.01 M

Experiments:

NameSampleSample stateSample conditions
1H-15N HSQCall_samplesnot availableCond_1
HNCACBall_samplesnot availableCond_1
HNCAall_samplesnot availableCond_1
HNCOall_samplesnot availableCond_1
HN(CO)CAall_samplesnot availableCond_1
HN(CA)COall_samplesnot availableCond_1

Software:

NMRPipe v97.027.12.56 - Raw spectral data processing

NMRView v5.0.4 - Data analysis

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

BMRB 6275 6445

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks