BMRB Entry 6243

Name: NMR Relaxation data for Protein Azurin from Pseudomonas aeruginosa
Published: 2004-09-20

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR6243

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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

NMR Relaxation data for Protein Azurin from Pseudomonas aeruginosa

Deposition date:

2004-06-11

Original release date:

2004-09-20

Authors:

Zhuravleva, Anastasia; Korzhnev, Dmitry; Kupce, Eriks; Billeter, Martin; Arseniev, Alexander; Orekhov, Vladislav

Citation:

Citation: Zhuravleva, Anastasia; Korzhnev, Dmitry; Kupce, Eriks; Arseniev, Alexander; Billeter, Martin; Orekhov, Vladislav. "Gated electron transfers and electron pathways in azurin: a NMR dynamic study at multiple fields and temperatures" J. Mol. Biol. 342, 1599-1611 (2004).
PubMed: 15364584

Assembly members:

Assembly members:
azurin, polymer, 128 residues, Formula weight is not available
CU1, non-polymer, 63.546 Da.

Natural source:

Natural source: Common Name: Pseudomonas aeruginosa Taxonomy ID: 287 Superkingdom: Eubacteria Kingdom: not available Genus/species: Pseudomonas aeruginosa

Experimental source:

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
azurin: AECSVDIQGNDQMQFNTNAI TVDKSCKQFTVNLSHPGNLP KNVMGHNWVLSTAADMQGVV TDGMASGLDKDYLKPDDSRV IAHTKLIGSGEKDSVTFDVS KLKEGEQYMFFCTFPGHSAL MKGTLTLK

Data sets:

heteronucl_NOEs
heteronucl_T1_relaxation
heteronucl_T2_relaxation
order_parameters
- S2_parameters_1
- S2_parameters_2

Data type	Count
order parameters	230
T1 relaxation values	806
T2 relaxation values	806

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	azurin	1
2	COPPER (I) ION	2

Entities:

Entity 1, azurin 128 residues - Formula weight is not available

1

ALA

GLU

CYS

SER

VAL

ASP

ILE

GLN

GLY

ASN

2

ASP

GLN

MET

GLN

PHE

ASN

THR

ASN

ALA

ILE

3

THR

VAL

ASP

LYS

SER

CYS

LYS

GLN

PHE

THR

4

VAL

ASN

LEU

SER

HIS

PRO

GLY

ASN

LEU

PRO

5

LYS

ASN

VAL

MET

GLY

HIS

ASN

TRP

VAL

LEU

6

SER

THR

ALA

ASP

MET

GLN

GLY

VAL

7

THR

ASP

GLY

MET

ALA

SER

GLY

LEU

ASP

LYS

8

ASP

TYR

LEU

LYS

PRO

ASP

SER

ARG

VAL

9

ILE

ALA

HIS

THR

LYS

LEU

ILE

GLY

SER

GLY

10

GLU

LYS

ASP

SER

VAL

THR

PHE

ASP

VAL

SER

11

LYS

LEU

LYS

GLU

GLY

GLU

GLN

TYR

MET

PHE

12

PHE

CYS

THR

PHE

PRO

GLY

HIS

SER

ALA

LEU

13

MET

LYS

GLY

THR

LEU

THR

LEU

LYS

Entity 2, COPPER (I) ION - Cu - 63.546 Da.

1

CU1

Samples:

sample_1: azurin, [U-95% 15N], 1 – 1 mM

condition_one: pH: 5.5; temperature: 289 K

condition_two: pH: 5.5; temperature: 317 K

Experiments:

Name	Sample	Sample state	Sample conditions
15N-1H HSQC	sample_1	not available	not available
15N T2	sample_1	not available	not available
1H-15N NOE	sample_1	not available	not available

Software:

NMRPipe -

DASHA -

MUNIN -

NMR spectrometers:

Varian INOVA 500 MHz
Varian INOVA 600 MHz
Varian INOVA 800 MHz
Varian INOVA 900 MHz

BMRB	1210 1211 1212 1213 1214 1215 1216 1217 1218 1219 18254 4465
PDB	1AG0 1AZN 1AZR 1AZU 1BEX 1E5Y 1E5Z 1E65 1E67 1ETJ 1EZL 1GR7 1I53 1ILS 1ILU 1JVL 1JVO 1JZE 1JZF 1JZG 1JZH 1JZI 1JZJ 1NZR 1VLX 1XB3 1XB6 1XB8 2AZU 2FNW 2GHZ 2GI0 2I7S 2IDF 2IWE 2OJ1 2TSA 2TSB 3AZU 3FPY 3FQ1 3FQ2 3FQY 3IBO 3IN0 3IN2 3JT2 3JTB 3N2J 3NP3 3NP4 3OQR 3UGE 4AZU 4BWW 4HZ1 4JKN 4KO6 4KO7 4KO9 4KOB 4KOC 4MFH 4QKT 4QLW 5AZU
DBJ	BAK87490 BAP24727 BAP53499 GAA20615
EMBL	CAA30279 CAW30062 CCQ86145 CDH73668 CDH79986
GB	AAA25730 AAG08307 AAP03090 AAT49489 ABJ14307
PRF	671048A
REF	NP_253609 WP_003095591 WP_003141697 WP_012077620 WP_031690945
SP	B3EWN9 P00282
AlphaFold	B3EWN9 P00282