BMRB Entry 18254

Name: 1H and 15N resonance assignments for Pseudomonas aeruginosa apo-azurin
Published: 2012-09-14

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR18254

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H and 15N resonance assignments for Pseudomonas aeruginosa apo-azurin

Deposition date:

2012-02-10

Original release date:

2012-09-14

Authors:

Zaballa, Maria-Eugenia; Abriata, Luciano; Donaire, Antonio; Vila, Alejandro

Citation:

Citation: Zaballa, Maria-Eugenia; Abriata, Luciano; Donaire, Antonio; Vila, Alejandro. "Flexibility of the metal-binding region in apo-cupredoxins." Proc. Natl. Acad. Sci. U.S.A. 109, 9254-9259 (2012).
PubMed: 22645370

Assembly members:

Assembly members:
apo-azurin, polymer, 128 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Pseudomonas aeruginosa Taxonomy ID: 287 Superkingdom: Bacteria Kingdom: not available Genus/species: Pseudomonas aeruginosa

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: (NA)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
apo-azurin: AECSVDIQGNDQMQFNTNAI TVDKSCKQFTVNLSHPGNLP KNVMGHNWVLSTAADMQGVV TDGMASGLDKDYLKPDDSRV IAHTKLIGSGEKDSVTFDVS KLKEGEQYMFFCTFPGHSAL MKGTLTLK

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
15N chemical shifts	107
1H chemical shifts	107

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	apo-azurin	1

Entities:

Entity 1, apo-azurin 128 residues - Formula weight is not available

1

ALA

GLU

CYS

SER

VAL

ASP

ILE

GLN

GLY

ASN

2

ASP

GLN

MET

GLN

PHE

ASN

THR

ASN

ALA

ILE

3

THR

VAL

ASP

LYS

SER

CYS

LYS

GLN

PHE

THR

4

VAL

ASN

LEU

SER

HIS

PRO

GLY

ASN

LEU

PRO

5

LYS

ASN

VAL

MET

GLY

HIS

ASN

TRP

VAL

LEU

6

SER

THR

ALA

ASP

MET

GLN

GLY

VAL

7

THR

ASP

GLY

MET

ALA

SER

GLY

LEU

ASP

LYS

8

ASP

TYR

LEU

LYS

PRO

ASP

SER

ARG

VAL

9

ILE

ALA

HIS

THR

LYS

LEU

ILE

GLY

SER

GLY

10

GLU

LYS

ASP

SER

VAL

THR

PHE

ASP

VAL

SER

11

LYS

LEU

LYS

GLU

GLY

GLU

GLN

TYR

MET

PHE

12

PHE

CYS

THR

PHE

PRO

GLY

HIS

SER

ALA

LEU

13

MET

LYS

GLY

THR

LEU

THR

LEU

LYS

Samples:

sample_1: apoAzurin, [U-100% 13C; U-100% 15N], 1 mM; DTT 2 mM; potassium phosphate 100 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0.1 M; pH: 7; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection, processing

CARA, Rochus Keller - chemical shift assignment, data analysis

SPARKY, Goddard - data analysis

NMR spectrometers:

Bruker Avance 600 MHz

Related Database Links:

BMRB	1210 1211 1212 1213 1214 1215 1216 1217 1218 1219 4465
PDB	1AG0 1AZN 1AZR 1AZU 1BEX 1E5Y 1E5Z 1E65 1E67 1ETJ 1EZL 1GR7 1I53 1ILS 1ILU 1JVL 1JVO 1JZE 1JZF 1JZG 1JZH 1JZI 1JZJ 1NZR 1VLX 1XB3 1XB6 1XB8 2AZU 2FNW 2GHZ 2GI0 2I7S 2IDF 2IWE 2OJ1 2TSA 2TSB 3AZU 3FPY 3FQ1 3FQ2 3FQY 3IBO 3IN0 3IN2 3JT2 3JTB 3N2J 3NP3 3NP4 3OQR 3UGE 4AZU 4BWW 4HZ1 4JKN 4KO6 4KO7 4KO9 4KOB 4KOC 4MFH 4QKT 4QLW 5AZU
DBJ	BAK87490 BAP24727 BAP53499 BAQ42761 BAR70381
EMBL	CAA30279 CAW30062 CCQ86145 CDH73668 CDH79986
GB	AAA25730 AAG08307 AAP03090 AAT49489 ABJ14307
PRF	671048A
REF	NP_253609 WP_003095591 WP_003141697 WP_012077620 WP_031690945
SP	B3EWN9 P00282
AlphaFold	B3EWN9 P00282

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks