BMRB Entry 1217
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR1217
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Citation: van de Kamp, Mart; Hali, Frits; Rosato, Nicola; Finazzi Agro, Alessandro; Canters, Gerard. "Purification and characterization of a non-reconstitutable azurin, obtained by
heterologous expression of the Pseudomonas aeruginosa azu gene in Escherichia
coli" Biochim. Biophys. Acta 1019, 283-292 (1990).
Assembly members:
azurin, polymer, 128 residues, Formula weight is not available
Natural source: Common Name: not available Taxonomy ID: 287 Superkingdom: Bacteria Kingdom: not available Genus/species: Pseudomonas aeruginosa
Experimental source: Production method: not available
Entity Sequences (FASTA):
azurin: AECSVDIQGNDQMQFNTNAI
TVDKSCKQFTVNLSHPGNLP
KNVMGHNWVLSTAADMQGVV
TDGMASGLDKDYLKPDDSRV
IAHTKLIGSGEKDSVTFDVS
KLKEGEQYMFFCTFPGHSAL
MKGTLTLK
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 16 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | azurin | 1 |
Entities:
Entity 1, azurin 128 residues - Formula weight is not available
| 1 | ALA | GLU | CYS | SER | VAL | ASP | ILE | GLN | GLY | ASN | ||||
| 2 | ASP | GLN | MET | GLN | PHE | ASN | THR | ASN | ALA | ILE | ||||
| 3 | THR | VAL | ASP | LYS | SER | CYS | LYS | GLN | PHE | THR | ||||
| 4 | VAL | ASN | LEU | SER | HIS | PRO | GLY | ASN | LEU | PRO | ||||
| 5 | LYS | ASN | VAL | MET | GLY | HIS | ASN | TRP | VAL | LEU | ||||
| 6 | SER | THR | ALA | ALA | ASP | MET | GLN | GLY | VAL | VAL | ||||
| 7 | THR | ASP | GLY | MET | ALA | SER | GLY | LEU | ASP | LYS | ||||
| 8 | ASP | TYR | LEU | LYS | PRO | ASP | ASP | SER | ARG | VAL | ||||
| 9 | ILE | ALA | HIS | THR | LYS | LEU | ILE | GLY | SER | GLY | ||||
| 10 | GLU | LYS | ASP | SER | VAL | THR | PHE | ASP | VAL | SER | ||||
| 11 | LYS | LEU | LYS | GLU | GLY | GLU | GLN | TYR | MET | PHE | ||||
| 12 | PHE | CYS | THR | PHE | PRO | GLY | HIS | SER | ALA | LEU | ||||
| 13 | MET | LYS | GLY | THR | LEU | THR | LEU | LYS |
Samples:
sample_one:
sample_condition_set_one: pH: 10 na; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| not available | sample_one | not available | sample_condition_set_one |
Software:
No software information available
NMR spectrometers:
- unknown unknown 0 MHz
Related Database Links:
| BMRB | 1210 1211 1212 1213 1214 1215 1216 1218 1219 18254 4465 |
| PDB | |
| DBJ | BAK87490 BAP24727 BAP53499 BAQ42761 BAR70381 |
| EMBL | CAA30279 CAW30062 CCQ86145 CDH73668 CDH79986 |
| GB | AAA25730 AAG08307 AAP03090 AAT49489 ABJ14307 |
| PRF | 671048A |
| REF | NP_253609 WP_003095591 WP_003141697 WP_012077620 WP_031690945 |
| SP | B3EWN9 P00282 |
| AlphaFold | B3EWN9 P00282 |