BMRB Entry 50852

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50852

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Title:
Sequential backbone assignment of mature a-Synuclein fibrils prepared from a-helical intermediate species
Deposition date:
2021-03-26
Original release date:
2021-04-19
Authors:
Ahlawat, Sahil; Mehra, Surabhi; Gowda, Chandrakala; Maji, Samir; Agarwal, Vipin
Citation:

Citation: Mehra, Surabhi; Ahlawat, Sahil; Kumar, Harish; Datta, Debalina; Navalkar, Ambuja; Singh, Nitu; Patel, Komal; Gadhe, Laxmikant; Kadu, Pradeep; Kumar, Rakesh; Jha, Narendra; Sakunthala, Arunima; Sawner, Ajay; Padinhateeri, Ranjith; Udgaonkar, Jayant; Agarwal, Vipin; Maji, Samir. "a-Synuclein Aggregation Intermediates form Fibril Polymorphs with Distinct Prion-like Properties"  J. Mol. Biol. 434, 167761-167761 (2022).
PubMed: 35907572

Assembly members:

Assembly members:
entity_1, polymer, 140 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pRK172

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MDVFMKGLSKAKEGVVAAAE KTKQGVAEAAGKTKEGVLYV GSKTKEGVVHGVATVAEKTK EQVTNVGGAVVTGVTAVAQK TVEGAGSIAAATGFVKKDQL GKNEEGAPQEGILEDMPVDP DNEAYEMPSEEGYQDYEPEA

Data sets:
Data typeCount
13C chemical shifts261
15N chemical shifts81

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Alpha synuclein1

Entities:

Entity 1, Alpha synuclein 140 residues - Formula weight is not available

1   METASPVALPHEMETLYSGLYLEUSERLYS
2   ALALYSGLUGLYVALVALALAALAALAGLU
3   LYSTHRLYSGLNGLYVALALAGLUALAALA
4   GLYLYSTHRLYSGLUGLYVALLEUTYRVAL
5   GLYSERLYSTHRLYSGLUGLYVALVALHIS
6   GLYVALALATHRVALALAGLULYSTHRLYS
7   GLUGLNVALTHRASNVALGLYGLYALAVAL
8   VALTHRGLYVALTHRALAVALALAGLNLYS
9   THRVALGLUGLYALAGLYSERILEALAALA
10   ALATHRGLYPHEVALLYSLYSASPGLNLEU
11   GLYLYSASNGLUGLUGLYALAPROGLNGLU
12   GLYILELEUGLUASPMETPROVALASPPRO
13   ASPASNGLUALATYRGLUMETPROSERGLU
14   GLUGLYTYRGLNASPTYRGLUPROGLUALA

Samples:

sample_1: alpha synuclein, [U-100% 13C; U-100% 15N], 11 mg; glycine 20 mM; sodium azide 0.01%; H2O 100%

sample_conditions_1: pH: 7.2; pressure: 1 atm; temperature: 280 K

Experiments:

NameSampleSample stateSample conditions
2D DARRsample_1isotropicsample_conditions_1
NCACX DARRsample_1isotropicsample_conditions_1
NCOCX DARRsample_1isotropicsample_conditions_1
CONCAsample_1isotropicsample_conditions_1
2D NCAsample_1isotropicsample_conditions_1
2D NCOsample_1isotropicsample_conditions_1
3D NCACBsample_1isotropicsample_conditions_1
2D 13C INEPTsample_1isotropicsample_conditions_1

Software:

CcpNmr Analysis v2.4.2 - chemical shift assignment, peak picking

TOPSPIN v3.5 - collection, processing

NMR spectrometers:

  • Bruker AVANCE III 700 MHz

Related Database Links:

BMRB 16300 16302 16342 16543 16546 16547 16548 16904 16939 17214 17498 17648 17649 17654 17665 17910 18207 18208 18243 18857 18860 19257 19337 19338 19344 19345 19350 19351 25227 25228 25518
PDB
DBJ BAF82858 BAG73790
EMBL CAG33339 CAG46454
GB AAA16117 AAC02114 AAG30302 AAH13293 AAI08276
REF NP_000336 NP_001009158 NP_001129014 NP_001139526 NP_001139527
SP P37840 P61139 P61140 P61142 P61143