BMRB Entry 17910

Title:
WT alpha-synuclein fibrils in the presence of phospholipid vesicles
Deposition date:
2011-09-01
Original release date:
2012-02-28
Authors:
Comellas, Gemma; Lemkau, Luisel; Zhou, Donghua; George, Julia; Rienstra, Chad
Citation:

Citation: Comellas, Gemma; Lemkau, Luisel Rodriguez; Zhou, Donghua; George, Julia; Rienstra, Chad Michael. "Structural intermediates during -synuclein fibrillogenesis on phospholipid vesicles."  J. Am. Chem. Soc. 134, 5090-5099 (2012).
PubMed: 22352310

Assembly members:

Assembly members:
wild-type_alpha-synuclein_formed_in_phospholipid_vesicles, polymer, 140 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: Bl21

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts180
15N chemical shifts51

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1alpha-synuclein1

Entities:

Entity 1, alpha-synuclein 140 residues - Formula weight is not available

1   METASPVALPHEMETLYSGLYLYSSERLYS
2   ALALYSGLUGLYVALVALALAALAALAGLU
3   LYSTHRLYSGLNGLYVALALAGLUALAALA
4   GLYLYSTHRLYSGLUGLYVALLEUTYRVAL
5   GLYSERLYSTHRLYSGLUGLYVALVALHIS
6   GLYVALALATHRVALALAGLULYSTHRLYS
7   GLUGLNVALTHRASNVALGLYGLYALAVAL
8   VALTHRGLYVALTHRALAVALALAGLNLYS
9   THRVALGLUGLYALAGLYSERILEALAALA
10   ALATHRGLYPHEVALLYSLYSASPGLNLEU
11   GLYLYSASNGLUGLUGLYALAPROGLNGLU
12   GLYILELEUGLUASPMETPROVALASPPRO
13   ASPASNGLUALATYRGLUMETPROSERGLU
14   GLUGLYTYRGLNASPTYRGLUPROGLUALA

Samples:

sample_1: Alpha-synuclein fibrils, [U-100% 13C; U-100% 15N], 64%; Phosphate buffer 50 mM; H2O 36%

sample_conditions_1: ionic strength: 50 mM; pH: 7.4; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
NCACXsample_1isotropicsample_conditions_1
NCOCXsample_1isotropicsample_conditions_1
CANCOsample_1isotropicsample_conditions_1

Software:

SPARKY, Goddard - chemical shift assignment

VNMRJ, Varian - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

  • Varian VNMRS 500 MHz

Related Database Links:

BMRB 16300 16302 16342 16543 16546 16547 16548 16904 16939 17214 17498 17648 17649 17654 17665 18207 18208 18243 18857 18860 19257 19337 19338 19344 19345 19350 19351 25227 25228
PDB
DBJ BAF82858 BAG73790
EMBL CAG33339 CAG46454
GB AAA16117 AAC02114 AAG30302 AAH13293 AAI08276
REF NP_000336 NP_001009158 NP_001032222 NP_001129014 NP_001139526
SP P37840 P61139 P61140 P61142 P61143
AlphaFold P61140 P37840 P61139 P61142 P61143