BMRB Entry 17665

Title:
human alpha synuclein construct   PubMed: 22006323
Deposition date:
2011-05-25
Original release date:
2011-10-19
Authors:
Pochapsky, Thomas; Perovic, Iva
Citation:

Citation: Wang, Wei; Perovic, Iva; Chittuluru, Johnathan; Kaganovich, Alice; Nguyen, Linh; Liao, Jingling; Auclair, Jared; Johnson, Derrick; Landeru, Anuradha; Simorellis, Alana; Ju, Shulin; Cookson, Mark; Asturias, Francisco; Agar, Jeffrey; Webb, Brian; Kang, Chulhee; Ringe, Dagmar; Petsko, Gregory; Pochapsky, Thomas; Hoang, Quyen. "A soluble -synuclein construct forms a dynamic tetramer."  Proc. Natl. Acad. Sci. U.S.A. 108, 17797-17802 (2011).

Assembly members:

Assembly members:
aSyn, polymer, 150 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: N/A

Experimental source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: N/A

Data sets:
Data typeCount
13C chemical shifts260
15N chemical shifts134
1H chemical shifts660

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1alpha synuclein, chain 11
2alpha synuclein, chain 21
3alpha synuclein, chain 31
4alpha synuclein, chain 41

Entities:

Entity 1, alpha synuclein, chain 1 150 residues - Formula weight is not available

1   GLYPROLEUGLYSERPROGLUPHEPROGLY
2   METASPVALPHEMETLYSGLYLEUSERLYS
3   ALALYSGLUGLYVALVALALAALAALAGLU
4   LYSTHRLYSGLNGLYVALALAGLUALAALA
5   GLYLYSTHRLYSGLUGLYVALLEUTYRVAL
6   GLYSERLYSTHRLYSGLUGLYVALVALHIS
7   GLYVALALATHRVALALAGLULYSTHRLYS
8   GLUGLNVALTHRASNVALGLYGLYALAVAL
9   VALTHRGLYVALTHRALAVALALAGLNLYS
10   THRVALGLUGLYALAGLYSERILEALAALA
11   ALATHRGLYPHEVALLYSLYSASPGLNLEU
12   GLYLYSASNGLUGLUGLYALAPROGLNGLU
13   GLYILELEUGLUASPMETPROVALASPPRO
14   ASPASNGLUALATYRGLUMETPROSERGLU
15   GLUGLYTYRGLNASPTYRGLUPROGLUALA

Samples:

sample_1: aSyn, [U-99% 13C; U-99% 15N], 0.5 mM; HEPES 100 mM; NaCl 150 mM; Glycerol 10%; n-octyl-beta-glucopyranoside (BOG) 0.1%; H2O 90%; D2O 10%

sample_2: aSyn, [U-99% 15N], 0.5 mM; HEPES 100 mM; NaCl 150 mM; Glycerol 10%; n-octyl-beta-glucopyranoside BOG 0.1%; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 150 mM; pH: 7.4; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_2isotropicsample_conditions_1
3D 1H-15N NOESYsample_2isotropicsample_conditions_1
3D 1H-15N TOCSYsample_2isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
15N-filtered 2D 1H-1H TOCSYsample_2isotropicsample_conditions_1

Software:

SPARKY, Goddard - data analysis

TOPSPIN, Bruker Biospin - processing

NMR spectrometers:

  • Bruker Avance 800 MHz

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts