BMRB Entry 25228

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR25228

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Title:
1H, 13C, and 15N chemical shift assignments for alpha-synuclein (H50Q)
Deposition date:
2014-09-15
Original release date:
2015-09-04
Authors:
Waudby, Christopher; Proukakis, Christos; Porcari, Riccardo; Bellotti, Vittorio; Shapira, Anthony; Christodoulou, John; Camilloni, Carlo; Tartaglia, Gian Gaetano; Stoppini, Monica; Verona, Guglielmo; Vendruscolo, Michele; Relini, Annalisa; Penco, Amanda; Cabrita, Lisa; Mullin, Stephen; Paton, Jack; Mangione, Patrizia; Bolognesi, Benedetta
Citation:

Citation: Porcari, Riccardo; Proukakis, Christos; Waudby, Christopher; Bolognesi, Benedetta; Mangione, Patrizia; Paton, Jack; Mullin, Stephen; Cabrita, Lisa; Penco, Amanda; Relini, Annalisa; Verona, Guglielmo; Vendruscolo, Michele; Stoppini, Monica; Tartaglia, Gian Gaetano; Camilloni, Carlo; Christodoulou, John; Shapira, Anthony; Bellotti, Vittorio. "The H50Q mutation induces a 10-fold decrease in the solubility of alpha-synuclein"  J. Biol. Chem. 290, 2395-2404 (2015).
PubMed: 25505181

Assembly members:

Assembly members:
H50Q, polymer, 140 residues, 14442.0329 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pT7.7

Entity Sequences (FASTA):

Entity Sequences (FASTA):
H50Q: MDVFMKGLSKAKEGVVAAAE KTKQGVAEAAGKTKEGVLYV GSKTKEGVVQGVATVAEKTK EQVTNVGGAVVTGVTAVAQK TVEGAGSIAAATGFVKKDQL GKNEEGAPQEGILEDMPVDP DNEAYEMPSEEGYQDYEPEA

Data sets:
Data typeCount
13C chemical shifts399
15N chemical shifts131
1H chemical shifts275

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1H50Q1

Entities:

Entity 1, H50Q 140 residues - 14442.0329 Da.

1   METASPVALPHEMETLYSGLYLEUSERLYS
2   ALALYSGLUGLYVALVALALAALAALAGLU
3   LYSTHRLYSGLNGLYVALALAGLUALAALA
4   GLYLYSTHRLYSGLUGLYVALLEUTYRVAL
5   GLYSERLYSTHRLYSGLUGLYVALVALGLN
6   GLYVALALATHRVALALAGLULYSTHRLYS
7   GLUGLNVALTHRASNVALGLYGLYALAVAL
8   VALTHRGLYVALTHRALAVALALAGLNLYS
9   THRVALGLUGLYALAGLYSERILEALAALA
10   ALATHRGLYPHEVALLYSLYSASPGLNLEU
11   GLYLYSASNGLUGLUGLYALAPROGLNGLU
12   GLYILELEUGLUASPMETPROVALASPPRO
13   ASPASNGLUALATYRGLUMETPROSERGLU
14   GLUGLYTYRGLNASPTYRGLUPROGLUALA

Samples:

sample_1: H50Q, [U-13C; U-15N], 0.7 mM; sodium phosphate 10.0 mM; NaCl 100.0 mM; NaN3 1e-07 kg/m3; DSS 1e-08 kg/m3; H2O 95%; D2O 5%

sample_conditions_1: pH: 7.500; pressure: 1.000 atm; temperature: 283.000 K

Experiments:

NameSampleSample stateSample conditions
3D HA(CO)NHsample_1isotropicsample_conditions_1
3D BEST-HNCACBsample_1isotropicsample_conditions_1
3D BEST-HNCOCACBsample_1isotropicsample_conditions_1
3D HNHAsample_1isotropicsample_conditions_1
3D BEST-HNCOsample_1isotropicsample_conditions_1
3D BEST-iHNCOsample_1isotropicsample_conditions_1
2D 1H-15N HSQC/HMQCsample_1isotropicsample_conditions_1

Software:

CcpNmr_Analysis v2.4, CCPN - assignment, peak picking

TopSpin v2.1, Bruker - acquisition

nmrPipe v7.5, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

  • Bruker Avance 700 MHz

Related Database Links:

BMRB 16300 16302 16342 16543 16546 16547 16548 16904 16939 17214 17498 17648 17649 17654 17665 17910 18207 18208 18243 18857 18860 19257 19337 19338 19344 19345 19350 19351 25227
PDB
DBJ BAF82858 BAG73790
EMBL CAG33339 CAG46454
GB AAA16117 AAC02114 AAG30302 AAH13293 AAI08276
REF NP_000336 NP_001009158 NP_001032222 NP_001129014 NP_001139526
SP P37840 P61139 P61140 P61142 P61143
AlphaFold P37840 P61139 P61140 P61142 P61143

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks