BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 17024

Title: Solution structure of the non-covalent complex of the ZNF216 A20 domain with ubiquitin

Deposition date: 2010-06-29 Original release date: 2012-08-02

Authors: Garner, Thomas; Long, Jed; Searle, Mark; Layfield, Robert

Citation: Garner, Thomas; Strachan, Joanna; Long, Jed; Layfield, Robert; Searle, Mark. "Co-localisation of ubiquitin receptors ZNF216 and p62 in a ubiquitin-mediated ternary complex"  Nat. Struct. Biol. ., .-..

Assembly members:
ZNF216-A20, polymer, 62 residues, 3868.3 Da.
ubiquitin, polymer, 76 residues, 8017.1 Da.
ZN, non-polymer, 65.409 Da.

Natural source:   Common Name: not available   Taxonomy ID: 10116   Superkingdom: Metazoa   Kingdom: Rattus   Genus/species: norvegicus not available

Experimental source:   Production method: recombinant technology' 'Escherichia coli' . . . Escherichia coli 'c41 (DE3)' . . . . . . . . . . . . . . . PGEX-4T1 . . . 'Expresed as an N-terminal GST fusion seperated by a thrombin cleavage site

Entity Sequences (FASTA):
ZNF216-A20: GSMAQETNQTPGPMLCSTGC GFYGNPRTNGMCSVCYKEHL QRQQNSGRMSPMGTASGSNS PT
ubiquitin: MQIFVKTLTGKTITLEVESS DTIDNVKSKIQDKEGIPPDQ QRLIFAGKQLEDGRTLSDYN IQKESTLHLVLRLRGG

Data sets:
Data typeCount
13C chemical shifts178
15N chemical shifts65
1H chemical shifts210

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