BMRB Entry 4737

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4737

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Title:
The Structure of the Transcriptional Antiterminator NusB from Escherichia Coli
Deposition date:
2000-05-10
Original release date:
2000-06-13
Authors:
Altieri, Amanda; Mazzulla, Marie; Horita, David; Coats, R.; Wingfield, Paul; Das, Asis; Court, Donald; Byrd, R.
Citation:

Citation: Altieri, Amanda; Mazzulla, Marie; Horita, David; Coats, R.; Wingfield, Paul; Das, Asis; Court, Donald; Byrd, R.. "The Structure of the Transcriptional Antiterminator NusB from Escherichia Coli"  Nat. Struct. Biol. 7, 470-474 (2000).

Assembly members:

Assembly members:
NusB, polymer, 139 residues, 15691 Da.

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
NusB: MKPAARRRARECAVQALYSW QLSQNDIADVEYQFLAEQDV KDVDVLYFRELLAGVATNTA YLDGLMKPYLSRLLEELGQV EKAVLRIALYELSKRSDVPY KVAINEAIELAKSFGAEDSH KFVNGVLDKAAPVIRPNKK

Data sets:
Data typeCount
1H chemical shifts969
13C chemical shifts474
15N chemical shifts136

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1NusB1

Entities:

Entity 1, NusB 139 residues - 15691 Da.

1   METLYSPROALAALAARGARGARGALAARG
2   GLUCYSALAVALGLNALALEUTYRSERTRP
3   GLNLEUSERGLNASNASPILEALAASPVAL
4   GLUTYRGLNPHELEUALAGLUGLNASPVAL
5   LYSASPVALASPVALLEUTYRPHEARGGLU
6   LEULEUALAGLYVALALATHRASNTHRALA
7   TYRLEUASPGLYLEUMETLYSPROTYRLEU
8   SERARGLEULEUGLUGLULEUGLYGLNVAL
9   GLULYSALAVALLEUARGILEALALEUTYR
10   GLULEUSERLYSARGSERASPVALPROTYR
11   LYSVALALAILEASNGLUALAILEGLULEU
12   ALALYSSERPHEGLYALAGLUASPSERHIS
13   LYSPHEVALASNGLYVALLEUASPLYSALA
14   ALAPROVALILEARGPROASNLYSLYS

Samples:

sample_1: NusB, [U-15N], 0.7 – 1.2 mM

sample_2: NusB, [U-2H; U-15N], 0.7 – 1.2 mM

sample_3: NusB, [U-15N; U-13C], 0.7 – 1.2 mM

Ex-cond_1: pH: 6.8; temperature: 298 K; ionic strength: 0.10 M

Experiments:

NameSampleSample stateSample conditions
3D CBCA(CO)NHnot availablenot availableEx-cond_1
3D HNCACBnot availablenot availableEx-cond_1
3D C(CO)NHnot availablenot availableEx-cond_1
3D H(CCO)NHnot availablenot availableEx-cond_1
3D HCCH-TOCSYnot availablenot availableEx-cond_1
2D 1H-15N HSQCnot availablenot availableEx-cond_1
2D 1H-13C HSQCnot availablenot availableEx-cond_1
2D CB(CGCD)HDnot availablenot availableEx-cond_1
2D CB(CGCDCE)HEnot availablenot availableEx-cond_1
3D 1H-(15N,13C)-NOESY-HMQCnot availablenot availableEx-cond_1
4D 1H-15N-15N-1H NOESYnot availablenot availableEx-cond_1
4D 1H-13C-15N-1H NOESYnot availablenot availableEx-cond_1
4D 1H-13C-13C-1H NOESYnot availablenot availableEx-cond_1

Software:

NMRPipe v1.7 - Data processing

Ansig v3.3 - Data analysis, graphical display

NMR spectrometers:

  • Varian Unity Plus 600 MHz

Related Database Links:

BMRB 17526
PDB
DBJ BAB33892 BAE76196 BAG75962 BAI23787 BAI29258
EMBL CAA25289 CAA45737 CAC44764 CAP74950 CAQ30885
GB AAA24228 AAB40172 AAB95441 AAC73519 AAG54765
PRF 2111328A
REF NP_308496 NP_414950 NP_459413 NP_706304 WP_000801121
SP A7ZIG9 A7ZX68 A8AK38 A9MM45 A9MX13
AlphaFold A7ZIG9 A7ZX68 A8AK38 A9MM45 A9MX13

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks