BMRB Entry 17526

Title:
EcNusB bound to BoxA RNA   PubMed: 21652641
Deposition date:
2011-03-15
Original release date:
2011-06-30
Authors:
Stagno, Jason; Altieri, Amanda; Bubunenko, Mikhail; Tarasov, Sergey; Li, Jess; Court, Donald; Byrd, Andrew; Ji, Xinhua
Citation:

Citation: Stagno, Jason; Altieri, Amanda; Bubunenko, Mikhail; Tarasov, Sergey; Li, Jess; Court, Donald; Byrd, R. Andrew; Ji, Xinhua. "Structural basis for RNA recognition by NusB and NusE in the initiation of transcription antitermination."  Nucleic Acids Res. 39, 7803-7815 (2011).

Assembly members:

Assembly members:
NusB, polymer, 139 residues, Formula weight is not available
BoxA, polymer, . residues, Formula weight is not available

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:   Production method: chemical synthesis

Experimental source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:   Production method: chemical synthesis

Data sets:
Data typeCount
13C chemical shifts427
15N chemical shifts135
1H chemical shifts722

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1NusB1
2BoxA RNA2

Entities:

Entity 1, NusB 139 residues - Formula weight is not available

1   METLYSPROALAALAARGARGARGALAARG
2   GLUALAALAVALGLNALALEUTYRSERTRP
3   GLNLEUSERGLNASNASPILEALAASPVAL
4   GLUTYRGLNPHELEUALAGLUGLNASPVAL
5   LYSASPVALASPVALLEUTYRPHEARGGLU
6   LEULEUALAGLYVALALATHRASNTHRALA
7   TYRLEUASPGLYLEUMETLYSPROTYRLEU
8   SERARGLEULEUGLUGLULEUGLYGLNVAL
9   GLULYSALAVALLEUARGILEALALEUTYR
10   GLULEUSERLYSARGSERASPVALPROTYR
11   LYSVALALAILEASNGLUALAILEGLULEU
12   ALALYSSERPHEGLYALAGLUASPSERHIS
13   LYSPHEVALASNGLYVALLEUASPLYSALA
14   ALAPROVALILEARGPROASNLYSLYS

Entity 2, BoxA RNA - Formula weight is not available

1   UGCUCUUUAA
2   CA

Samples:

sample_1: E coli NusB, [U-100% 13C; U-100% 15N], 250 uM; boxA RNA, [U-100% 13C; U-100% 15N], 250 uM

sample_conditions_1: ionic strength: 0.02 M; pH: 6.5; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1

Software:

CcpNMR, CCPN - chemical shift assignment

NMR spectrometers:

  • Varian INOVA 600 MHz
  • Varian INOVA 800 MHz

Related Database Links:

BMRB 4737
PDB
DBJ BAB33892 BAE76196 BAG75962 BAI23787 BAI29258
EMBL CAA25289 CAA45737 CAC44764 CAP74950 CAQ30885
GB AAA24228 AAB40172 AAB95441 AAC73519 AAG54765
PRF 2111328A
REF NP_308496 NP_414950 NP_706304 WP_000801121 WP_000801122
SP A7ZIG9 A7ZX68 B1J033 B1LJG6 B1XF04
AlphaFold B1LJG6

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts