BMRB Entry 26997

Name: Backbone assignment of human 4E-BP1 (fragment 44 to 87) bound to mouse eIF4E
Published: 2017-08-02

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR26997

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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone assignment of human 4E-BP1 (fragment 44 to 87) bound to mouse eIF4E

Deposition date:

2017-01-18

Original release date:

2017-08-02

Authors:

Leger-Abraham, Melissa; Wagner, Gerhard; Boeszoermenyi, Andras; Arthanari, Haribabu; Sekiyama, Naotaka

Citation:

Citation: Sekiyama, Naotaka; Boeszoermenyi, Andras; Arthanari, Haribabu; Wagner, Gerhard; Leger-Abraham, Melissa. "(1)H, (13)C, and (15)N backbone chemical shift assignments of 4E-BP144-87 and 4E-BP144-87 bound to eIF4E" Biomol. NMR Assign. 11, 187-191 (2017).
PubMed: 28589219

Assembly members:

Assembly members:
4E-BP1_44to87, polymer, 47 residues, 5124.7813 Da.
eIF4E, polymer, 219 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET30

Entity Sequences (FASTA):

Entity Sequences (FASTA):
4E-BP1_44to87: GEFSTTPGGTRIIYDRKFLM ECRNSPVTKTPPRDLPTIPG VTSPSSD
eIF4E: GSMATVEPETTPTTNPPPAE EEKTESNQEVANPEHYIKHP LQNRWALWFFKNDKSKTWQA NLRLISKFDTVEDFWALYNH IQLSSNLMPGCDYSLFKDGI EPMWEDEKNKRGGRWLITLN KQQRRSDLDRFWLETLLCLI GESFDDYSDDVCGAVVNVRA KGDKIAIWTTECENRDAVTH IGRVYKERLGLPPKIVIGYQ SHADTATKSGSTTKNRFVV

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list

Data type	Count
13C chemical shifts	72
15N chemical shifts	36
1H chemical shifts	36

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	human 4E-BP1 (fragment 44 to 87)	1
2	eIF4E	2

Entities:

Entity 1, human 4E-BP1 (fragment 44 to 87) 47 residues - 5124.7813 Da.

1

GLY

GLU

PHE

SER

THR

PRO

GLY

THR

2

ARG

ILE

TYR

ASP

ARG

LYS

PHE

LEU

MET

3

GLU

CYS

ARG

ASN

SER

PRO

VAL

THR

LYS

THR

4

PRO

ARG

ASP

LEU

PRO

THR

ILE

PRO

GLY

5

VAL

THR

SER

PRO

SER

ASP

Entity 2, eIF4E 219 residues - Formula weight is not available

1

GLY

SER

MET

ALA

THR

VAL

GLU

PRO

GLU

THR

2

THR

PRO

THR

ASN

PRO

ALA

GLU

3

GLU

LYS

THR

GLU

SER

ASN

GLN

GLU

VAL

4

ALA

ASN

PRO

GLU

HIS

TYR

ILE

LYS

HIS

PRO

5

LEU

GLN

ASN

ARG

TRP

ALA

LEU

TRP

PHE

6

LYS

ASN

ASP

LYS

SER

LYS

THR

TRP

GLN

ALA

7

ASN

LEU

ARG

LEU

ILE

SER

LYS

PHE

ASP

THR

8

VAL

GLU

ASP

PHE

TRP

ALA

LEU

TYR

ASN

HIS

9

ILE

GLN

LEU

SER

ASN

LEU

MET

PRO

GLY

10

CYS

ASP

TYR

SER

LEU

PHE

LYS

ASP

GLY

ILE

11

GLU

PRO

MET

TRP

GLU

ASP

GLU

LYS

ASN

LYS

12

ARG

GLY

ARG

TRP

LEU

ILE

THR

LEU

ASN

13

LYS

GLN

ARG

SER

ASP

LEU

ASP

ARG

14

PHE

TRP

LEU

GLU

THR

LEU

CYS

LEU

ILE

15

GLY

GLU

SER

PHE

ASP

TYR

SER

ASP

16

VAL

CYS

GLY

ALA

VAL

ASN

VAL

ARG

ALA

17

LYS

GLY

ASP

LYS

ILE

ALA

ILE

TRP

THR

18

GLU

CYS

GLU

ASN

ARG

ASP

ALA

VAL

THR

HIS

19

ILE

GLY

ARG

VAL

TYR

LYS

GLU

ARG

LEU

GLY

20

LEU

PRO

LYS

ILE

VAL

ILE

GLY

TYR

GLN

21

SER

HIS

ALA

ASP

THR

ALA

THR

LYS

SER

GLY

22

SER

THR

LYS

ASN

ARG

PHE

VAL

Samples:

sample_1: 4E-BP1_44to87, [U-13C; U-15N], 0.25 mM; NaH2PO4/Na2HPO4 20 mM; NaCl 50 mM; DTT 2 mM

sample_conditions_1: ionic strength: 0.070 M; pH: 6.500; pressure: 1.000 atm; temperature: 298.000 K

Experiments:

Name	Sample	Sample state	Sample conditions
HNCO	sample_1	isotropic	sample_conditions_1
HNCA	sample_1	isotropic	sample_conditions_1
HSQC	sample_1	isotropic	sample_conditions_1
HNCACO	sample_1	isotropic	sample_conditions_1
HNCOCA	sample_1	isotropic	sample_conditions_1

Software:

XEASY v1.9.0, Keller and Wuthrich - chemical shift assignment

NMR spectrometers:

Bruker Avance 750 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks