BMRB Entry 26997
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR26997
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Citation: Sekiyama, Naotaka; Boeszoermenyi, Andras; Arthanari, Haribabu; Wagner, Gerhard; Leger-Abraham, Melissa. "(1)H, (13)C, and (15)N backbone chemical shift assignments of 4E-BP144-87 and 4E-BP144-87 bound to eIF4E" Biomol. NMR Assign. 11, 187-191 (2017).
PubMed: 28589219
Assembly members:
4E-BP1_44to87, polymer, 47 residues, 5124.7813 Da.
eIF4E, polymer, 219 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET30
Entity Sequences (FASTA):
4E-BP1_44to87: GEFSTTPGGTRIIYDRKFLM
ECRNSPVTKTPPRDLPTIPG
VTSPSSD
eIF4E: GSMATVEPETTPTTNPPPAE
EEKTESNQEVANPEHYIKHP
LQNRWALWFFKNDKSKTWQA
NLRLISKFDTVEDFWALYNH
IQLSSNLMPGCDYSLFKDGI
EPMWEDEKNKRGGRWLITLN
KQQRRSDLDRFWLETLLCLI
GESFDDYSDDVCGAVVNVRA
KGDKIAIWTTECENRDAVTH
IGRVYKERLGLPPKIVIGYQ
SHADTATKSGSTTKNRFVV
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 72 |
| 15N chemical shifts | 36 |
| 1H chemical shifts | 36 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | human 4E-BP1 (fragment 44 to 87) | 1 |
| 2 | eIF4E | 2 |
Entities:
Entity 1, human 4E-BP1 (fragment 44 to 87) 47 residues - 5124.7813 Da.
| 1 | GLY | GLU | PHE | SER | THR | THR | PRO | GLY | GLY | THR | ||||
| 2 | ARG | ILE | ILE | TYR | ASP | ARG | LYS | PHE | LEU | MET | ||||
| 3 | GLU | CYS | ARG | ASN | SER | PRO | VAL | THR | LYS | THR | ||||
| 4 | PRO | PRO | ARG | ASP | LEU | PRO | THR | ILE | PRO | GLY | ||||
| 5 | VAL | THR | SER | PRO | SER | SER | ASP |
Entity 2, eIF4E 219 residues - Formula weight is not available
| 1 | GLY | SER | MET | ALA | THR | VAL | GLU | PRO | GLU | THR | ||||
| 2 | THR | PRO | THR | THR | ASN | PRO | PRO | PRO | ALA | GLU | ||||
| 3 | GLU | GLU | LYS | THR | GLU | SER | ASN | GLN | GLU | VAL | ||||
| 4 | ALA | ASN | PRO | GLU | HIS | TYR | ILE | LYS | HIS | PRO | ||||
| 5 | LEU | GLN | ASN | ARG | TRP | ALA | LEU | TRP | PHE | PHE | ||||
| 6 | LYS | ASN | ASP | LYS | SER | LYS | THR | TRP | GLN | ALA | ||||
| 7 | ASN | LEU | ARG | LEU | ILE | SER | LYS | PHE | ASP | THR | ||||
| 8 | VAL | GLU | ASP | PHE | TRP | ALA | LEU | TYR | ASN | HIS | ||||
| 9 | ILE | GLN | LEU | SER | SER | ASN | LEU | MET | PRO | GLY | ||||
| 10 | CYS | ASP | TYR | SER | LEU | PHE | LYS | ASP | GLY | ILE | ||||
| 11 | GLU | PRO | MET | TRP | GLU | ASP | GLU | LYS | ASN | LYS | ||||
| 12 | ARG | GLY | GLY | ARG | TRP | LEU | ILE | THR | LEU | ASN | ||||
| 13 | LYS | GLN | GLN | ARG | ARG | SER | ASP | LEU | ASP | ARG | ||||
| 14 | PHE | TRP | LEU | GLU | THR | LEU | LEU | CYS | LEU | ILE | ||||
| 15 | GLY | GLU | SER | PHE | ASP | ASP | TYR | SER | ASP | ASP | ||||
| 16 | VAL | CYS | GLY | ALA | VAL | VAL | ASN | VAL | ARG | ALA | ||||
| 17 | LYS | GLY | ASP | LYS | ILE | ALA | ILE | TRP | THR | THR | ||||
| 18 | GLU | CYS | GLU | ASN | ARG | ASP | ALA | VAL | THR | HIS | ||||
| 19 | ILE | GLY | ARG | VAL | TYR | LYS | GLU | ARG | LEU | GLY | ||||
| 20 | LEU | PRO | PRO | LYS | ILE | VAL | ILE | GLY | TYR | GLN | ||||
| 21 | SER | HIS | ALA | ASP | THR | ALA | THR | LYS | SER | GLY | ||||
| 22 | SER | THR | THR | LYS | ASN | ARG | PHE | VAL | VAL |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks