data_26997 ####################### # Entry information # ####################### save_entry_information _Entry.Sf_category entry_information _Entry.Sf_framecode entry_information _Entry.ID 26997 _Entry.Title ; Backbone assignment of human 4E-BP1 (fragment 44 to 87) bound to mouse eIF4E ; _Entry.Type macromolecule _Entry.Version_type original _Entry.Submission_date 2017-01-18 _Entry.Accession_date 2017-01-18 _Entry.Last_release_date 2017-01-18 _Entry.Original_release_date 2017-01-18 _Entry.Origination author _Entry.Format_name . _Entry.NMR_STAR_version 3.2.0.16 _Entry.Original_NMR_STAR_version 3.1 _Entry.Experimental_method NMR _Entry.Experimental_method_subtype solution _Entry.Source_data_format . _Entry.Source_data_format_version . _Entry.Generated_software_name . _Entry.Generated_software_version . _Entry.Generated_software_ID . _Entry.Generated_software_label . _Entry.Generated_date . _Entry.DOI . _Entry.UUID . _Entry.Related_coordinate_file_name . _Entry.Details 'Backbone assignment of 4E-BP1 bound to mouse eIF4E' loop_ _Entry_author.Ordinal _Entry_author.Given_name _Entry_author.Family_name _Entry_author.First_initial _Entry_author.Middle_initials _Entry_author.Family_title _Entry_author.ORCID _Entry_author.Entry_ID 1 Melissa Leger-Abraham . . . . 26997 2 Gerhard Wagner . . . . 26997 3 Andras Boeszoermenyi . . . . 26997 4 Haribabu Arthanari . . . . 26997 5 Naotaka Sekiyama . . . . 26997 stop_ loop_ _SG_project.SG_project_ID _SG_project.Project_name _SG_project.Full_name_of_center _SG_project.Initial_of_center _SG_project.Entry_ID 1 'not applicable' 'not applicable' . 26997 stop_ loop_ _Entry_src.ID _Entry_src.Project_name _Entry_src.Organization_full_name _Entry_src.Organization_initials _Entry_src.Entry_ID 1 . 'Harvard Medical School' . 26997 stop_ loop_ _Data_set.Type _Data_set.Count _Data_set.Entry_ID assigned_chemical_shifts 1 26997 stop_ loop_ _Datum.Type _Datum.Count _Datum.Entry_ID '13C chemical shifts' 72 26997 '15N chemical shifts' 36 26997 '1H chemical shifts' 36 26997 stop_ loop_ _Release.Release_number _Release.Format_type _Release.Format_version _Release.Date _Release.Submission_date _Release.Type _Release.Author _Release.Detail _Release.Entry_ID 2 . . 2017-08-02 2017-01-18 update BMRB 'update entry citation' 26997 1 . . 2017-08-02 2017-01-18 original author 'original release' 26997 stop_ loop_ _Related_entries.Database_name _Related_entries.Database_accession_code _Related_entries.Relationship _Related_entries.Entry_ID BMRB 27003 'human 4E-BP1 (fragment 44 to 87) (apo form)' 26997 PDB 5BXV . 26997 stop_ save_ ############### # Citations # ############### save_entry_citation _Citation.Sf_category citations _Citation.Sf_framecode entry_citation _Citation.Entry_ID 26997 _Citation.ID 1 _Citation.Class 'entry citation' _Citation.CAS_abstract_code . _Citation.MEDLINE_UI_code . _Citation.DOI 10.1007/s12104-017-9744-9 _Citation.PubMed_ID 28589219 _Citation.Full_citation . _Citation.Title ; (1)H, (13)C, and (15)N backbone chemical shift assignments of 4E-BP144-87 and 4E-BP144-87 bound to eIF4E ; _Citation.Status published _Citation.Type journal _Citation.Journal_abbrev 'Biomol. NMR Assign.' _Citation.Journal_name_full 'Biomolecular NMR assignments' _Citation.Journal_volume 11 _Citation.Journal_issue 2 _Citation.Journal_ASTM . _Citation.Journal_ISSN 1874-270X _Citation.Journal_CSD . _Citation.Book_title . _Citation.Book_chapter_title . _Citation.Book_volume . _Citation.Book_series . _Citation.Book_publisher . _Citation.Book_publisher_city . _Citation.Book_ISBN . _Citation.Conference_title . _Citation.Conference_site . _Citation.Conference_state_province . _Citation.Conference_country . _Citation.Conference_start_date . _Citation.Conference_end_date . _Citation.Conference_abstract_number . _Citation.Thesis_institution . _Citation.Thesis_institution_city . _Citation.Thesis_institution_country . _Citation.WWW_URL . _Citation.Page_first 187 _Citation.Page_last 191 _Citation.Year 2017 _Citation.Details . loop_ _Citation_author.Ordinal _Citation_author.Given_name _Citation_author.Family_name _Citation_author.First_initial _Citation_author.Middle_initials _Citation_author.Family_title _Citation_author.ORCID _Citation_author.Entry_ID _Citation_author.Citation_ID 1 Naotaka Sekiyama N. . . . 26997 1 2 Andras Boeszoermenyi A. . . . 26997 1 3 Haribabu Arthanari H. . . . 26997 1 4 Gerhard Wagner G. . . . 26997 1 5 Melissa Leger-Abraham M. . . . 26997 1 stop_ save_ ############################################# # Molecular system (assembly) description # ############################################# save_assembly _Assembly.Sf_category assembly _Assembly.Sf_framecode assembly _Assembly.Entry_ID 26997 _Assembly.ID 1 _Assembly.Name 'human 4E-BP1 (fragment 44 to 87) bound to mouse eIF4E' _Assembly.BMRB_code . _Assembly.Number_of_components 2 _Assembly.Organic_ligands 0 _Assembly.Metal_ions 0 _Assembly.Non_standard_bonds no _Assembly.Ambiguous_conformational_states . _Assembly.Ambiguous_chem_comp_sites . _Assembly.Molecules_in_chemical_exchange . _Assembly.Paramagnetic no _Assembly.Thiol_state 'all free' _Assembly.Molecular_mass . _Assembly.Enzyme_commission_number . _Assembly.Details . _Assembly.DB_query_date . _Assembly.DB_query_revised_last_date . loop_ _Entity_assembly.ID _Entity_assembly.Entity_assembly_name _Entity_assembly.Entity_ID _Entity_assembly.Entity_label _Entity_assembly.Asym_ID _Entity_assembly.PDB_chain_ID _Entity_assembly.Experimental_data_reported _Entity_assembly.Physical_state _Entity_assembly.Conformational_isomer _Entity_assembly.Chemical_exchange_state _Entity_assembly.Magnetic_equivalence_group_code _Entity_assembly.Role _Entity_assembly.Details _Entity_assembly.Entry_ID _Entity_assembly.Assembly_ID 1 'human 4E-BP1 (fragment 44 to 87)' 1 $4E-BP1_44to87 A . yes native no no . . . 26997 1 2 eIF4E 2 $eIF4E B . no native no no . . . 26997 1 stop_ save_ #################################### # Biological polymers and ligands # #################################### save_4E-BP1_44to87 _Entity.Sf_category entity _Entity.Sf_framecode 4E-BP1_44to87 _Entity.Entry_ID 26997 _Entity.ID 1 _Entity.BMRB_code . _Entity.Name 4E-BP1_44to87 _Entity.Type polymer _Entity.Polymer_common_type . _Entity.Polymer_type polypeptide(L) _Entity.Polymer_type_details . _Entity.Polymer_strand_ID A _Entity.Polymer_seq_one_letter_code_can . _Entity.Polymer_seq_one_letter_code ; GEFSTTPGGTRIIYDRKFLM ECRNSPVTKTPPRDLPTIPG VTSPSSD ; _Entity.Target_identifier . _Entity.Polymer_author_defined_seq . _Entity.Polymer_author_seq_details . _Entity.Ambiguous_conformational_states no _Entity.Ambiguous_chem_comp_sites no _Entity.Nstd_monomer no _Entity.Nstd_chirality no _Entity.Nstd_linkage no _Entity.Nonpolymer_comp_ID . _Entity.Nonpolymer_comp_label . _Entity.Number_of_monomers 47 _Entity.Number_of_nonpolymer_components . _Entity.Paramagnetic no _Entity.Thiol_state 'all free' _Entity.Src_method . _Entity.Parent_entity_ID 1 _Entity.Fragment . _Entity.Mutation . _Entity.EC_number . _Entity.Calc_isoelectric_point . _Entity.Formula_weight 5124.7813 _Entity.Formula_weight_exptl . _Entity.Formula_weight_exptl_meth . _Entity.Details . _Entity.DB_query_date . _Entity.DB_query_revised_last_date . loop_ _Entity_comp_index.ID _Entity_comp_index.Auth_seq_ID _Entity_comp_index.Comp_ID _Entity_comp_index.Comp_label _Entity_comp_index.Entry_ID _Entity_comp_index.Entity_ID 1 -3 GLY . 26997 1 2 -2 GLU . 26997 1 3 -1 PHE . 26997 1 4 44 SER . 26997 1 5 45 THR . 26997 1 6 46 THR . 26997 1 7 47 PRO . 26997 1 8 48 GLY . 26997 1 9 49 GLY . 26997 1 10 50 THR . 26997 1 11 51 ARG . 26997 1 12 52 ILE . 26997 1 13 53 ILE . 26997 1 14 54 TYR . 26997 1 15 55 ASP . 26997 1 16 56 ARG . 26997 1 17 57 LYS . 26997 1 18 58 PHE . 26997 1 19 59 LEU . 26997 1 20 60 MET . 26997 1 21 61 GLU . 26997 1 22 62 CYS . 26997 1 23 63 ARG . 26997 1 24 64 ASN . 26997 1 25 65 SER . 26997 1 26 66 PRO . 26997 1 27 67 VAL . 26997 1 28 68 THR . 26997 1 29 69 LYS . 26997 1 30 70 THR . 26997 1 31 71 PRO . 26997 1 32 72 PRO . 26997 1 33 73 ARG . 26997 1 34 74 ASP . 26997 1 35 75 LEU . 26997 1 36 76 PRO . 26997 1 37 77 THR . 26997 1 38 78 ILE . 26997 1 39 79 PRO . 26997 1 40 80 GLY . 26997 1 41 81 VAL . 26997 1 42 82 THR . 26997 1 43 83 SER . 26997 1 44 84 PRO . 26997 1 45 85 SER . 26997 1 46 86 SER . 26997 1 47 87 ASP . 26997 1 stop_ loop_ _Entity_poly_seq.Hetero _Entity_poly_seq.Mon_ID _Entity_poly_seq.Num _Entity_poly_seq.Comp_index_ID _Entity_poly_seq.Entry_ID _Entity_poly_seq.Entity_ID . GLY 1 1 26997 1 . GLU 2 2 26997 1 . PHE 3 3 26997 1 . SER 4 4 26997 1 . THR 5 5 26997 1 . THR 6 6 26997 1 . PRO 7 7 26997 1 . GLY 8 8 26997 1 . GLY 9 9 26997 1 . THR 10 10 26997 1 . ARG 11 11 26997 1 . ILE 12 12 26997 1 . ILE 13 13 26997 1 . TYR 14 14 26997 1 . ASP 15 15 26997 1 . ARG 16 16 26997 1 . LYS 17 17 26997 1 . PHE 18 18 26997 1 . LEU 19 19 26997 1 . MET 20 20 26997 1 . GLU 21 21 26997 1 . CYS 22 22 26997 1 . ARG 23 23 26997 1 . ASN 24 24 26997 1 . SER 25 25 26997 1 . PRO 26 26 26997 1 . VAL 27 27 26997 1 . THR 28 28 26997 1 . LYS 29 29 26997 1 . THR 30 30 26997 1 . PRO 31 31 26997 1 . PRO 32 32 26997 1 . ARG 33 33 26997 1 . ASP 34 34 26997 1 . LEU 35 35 26997 1 . PRO 36 36 26997 1 . THR 37 37 26997 1 . ILE 38 38 26997 1 . PRO 39 39 26997 1 . GLY 40 40 26997 1 . VAL 41 41 26997 1 . THR 42 42 26997 1 . SER 43 43 26997 1 . PRO 44 44 26997 1 . SER 45 45 26997 1 . SER 46 46 26997 1 . ASP 47 47 26997 1 stop_ save_ save_eIF4E _Entity.Sf_category entity _Entity.Sf_framecode eIF4E _Entity.Entry_ID 26997 _Entity.ID 2 _Entity.BMRB_code . _Entity.Name eIF4E _Entity.Type polymer _Entity.Polymer_common_type . _Entity.Polymer_type polypeptide(L) _Entity.Polymer_type_details . _Entity.Polymer_strand_ID B _Entity.Polymer_seq_one_letter_code_can . _Entity.Polymer_seq_one_letter_code ; GSMATVEPETTPTTNPPPAE EEKTESNQEVANPEHYIKHP LQNRWALWFFKNDKSKTWQA NLRLISKFDTVEDFWALYNH IQLSSNLMPGCDYSLFKDGI EPMWEDEKNKRGGRWLITLN KQQRRSDLDRFWLETLLCLI GESFDDYSDDVCGAVVNVRA KGDKIAIWTTECENRDAVTH IGRVYKERLGLPPKIVIGYQ SHADTATKSGSTTKNRFVV ; _Entity.Target_identifier . _Entity.Polymer_author_defined_seq . _Entity.Polymer_author_seq_details . _Entity.Ambiguous_conformational_states no _Entity.Ambiguous_chem_comp_sites no _Entity.Nstd_monomer no _Entity.Nstd_chirality no _Entity.Nstd_linkage no _Entity.Nonpolymer_comp_ID . _Entity.Nonpolymer_comp_label . _Entity.Number_of_monomers 219 _Entity.Number_of_nonpolymer_components . _Entity.Paramagnetic no _Entity.Thiol_state 'all free' _Entity.Src_method . _Entity.Parent_entity_ID 2 _Entity.Fragment . _Entity.Mutation . _Entity.EC_number . _Entity.Calc_isoelectric_point . _Entity.Formula_weight . _Entity.Formula_weight_exptl . _Entity.Formula_weight_exptl_meth . _Entity.Details ; The "GS" residues do not belong to the native mouse eIF4E sequence. They come from the purification tag, which were cleaved following TEV cleavage, but remained appended to the mouse eIF4E sequence. ; _Entity.DB_query_date . _Entity.DB_query_revised_last_date . loop_ _Entity_comp_index.ID _Entity_comp_index.Auth_seq_ID _Entity_comp_index.Comp_ID _Entity_comp_index.Comp_label _Entity_comp_index.Entry_ID _Entity_comp_index.Entity_ID 1 . GLY . 26997 2 2 . SER . 26997 2 3 . MET . 26997 2 4 . ALA . 26997 2 5 . THR . 26997 2 6 . VAL . 26997 2 7 . GLU . 26997 2 8 . PRO . 26997 2 9 . GLU . 26997 2 10 . THR . 26997 2 11 . THR . 26997 2 12 . PRO . 26997 2 13 . THR . 26997 2 14 . THR . 26997 2 15 . ASN . 26997 2 16 . PRO . 26997 2 17 . PRO . 26997 2 18 . PRO . 26997 2 19 . ALA . 26997 2 20 . GLU . 26997 2 21 . GLU . 26997 2 22 . GLU . 26997 2 23 . LYS . 26997 2 24 . THR . 26997 2 25 . GLU . 26997 2 26 . SER . 26997 2 27 . ASN . 26997 2 28 . GLN . 26997 2 29 . GLU . 26997 2 30 . VAL . 26997 2 31 . ALA . 26997 2 32 . ASN . 26997 2 33 . PRO . 26997 2 34 . GLU . 26997 2 35 . HIS . 26997 2 36 . TYR . 26997 2 37 . ILE . 26997 2 38 . LYS . 26997 2 39 . HIS . 26997 2 40 . PRO . 26997 2 41 . LEU . 26997 2 42 . GLN . 26997 2 43 . ASN . 26997 2 44 . ARG . 26997 2 45 . TRP . 26997 2 46 . ALA . 26997 2 47 . LEU . 26997 2 48 . TRP . 26997 2 49 . PHE . 26997 2 50 . PHE . 26997 2 51 . LYS . 26997 2 52 . ASN . 26997 2 53 . ASP . 26997 2 54 . LYS . 26997 2 55 . SER . 26997 2 56 . LYS . 26997 2 57 . THR . 26997 2 58 . TRP . 26997 2 59 . GLN . 26997 2 60 . ALA . 26997 2 61 . ASN . 26997 2 62 . LEU . 26997 2 63 . ARG . 26997 2 64 . LEU . 26997 2 65 . ILE . 26997 2 66 . SER . 26997 2 67 . LYS . 26997 2 68 . PHE . 26997 2 69 . ASP . 26997 2 70 . THR . 26997 2 71 . VAL . 26997 2 72 . GLU . 26997 2 73 . ASP . 26997 2 74 . PHE . 26997 2 75 . TRP . 26997 2 76 . ALA . 26997 2 77 . LEU . 26997 2 78 . TYR . 26997 2 79 . ASN . 26997 2 80 . HIS . 26997 2 81 . ILE . 26997 2 82 . GLN . 26997 2 83 . LEU . 26997 2 84 . SER . 26997 2 85 . SER . 26997 2 86 . ASN . 26997 2 87 . LEU . 26997 2 88 . MET . 26997 2 89 . PRO . 26997 2 90 . GLY . 26997 2 91 . CYS . 26997 2 92 . ASP . 26997 2 93 . TYR . 26997 2 94 . SER . 26997 2 95 . LEU . 26997 2 96 . PHE . 26997 2 97 . LYS . 26997 2 98 . ASP . 26997 2 99 . GLY . 26997 2 100 . ILE . 26997 2 101 . GLU . 26997 2 102 . PRO . 26997 2 103 . MET . 26997 2 104 . TRP . 26997 2 105 . GLU . 26997 2 106 . ASP . 26997 2 107 . GLU . 26997 2 108 . LYS . 26997 2 109 . ASN . 26997 2 110 . LYS . 26997 2 111 . ARG . 26997 2 112 . GLY . 26997 2 113 . GLY . 26997 2 114 . ARG . 26997 2 115 . TRP . 26997 2 116 . LEU . 26997 2 117 . ILE . 26997 2 118 . THR . 26997 2 119 . LEU . 26997 2 120 . ASN . 26997 2 121 . LYS . 26997 2 122 . GLN . 26997 2 123 . GLN . 26997 2 124 . ARG . 26997 2 125 . ARG . 26997 2 126 . SER . 26997 2 127 . ASP . 26997 2 128 . LEU . 26997 2 129 . ASP . 26997 2 130 . ARG . 26997 2 131 . PHE . 26997 2 132 . TRP . 26997 2 133 . LEU . 26997 2 134 . GLU . 26997 2 135 . THR . 26997 2 136 . LEU . 26997 2 137 . LEU . 26997 2 138 . CYS . 26997 2 139 . LEU . 26997 2 140 . ILE . 26997 2 141 . GLY . 26997 2 142 . GLU . 26997 2 143 . SER . 26997 2 144 . PHE . 26997 2 145 . ASP . 26997 2 146 . ASP . 26997 2 147 . TYR . 26997 2 148 . SER . 26997 2 149 . ASP . 26997 2 150 . ASP . 26997 2 151 . VAL . 26997 2 152 . CYS . 26997 2 153 . GLY . 26997 2 154 . ALA . 26997 2 155 . VAL . 26997 2 156 . VAL . 26997 2 157 . ASN . 26997 2 158 . VAL . 26997 2 159 . ARG . 26997 2 160 . ALA . 26997 2 161 . LYS . 26997 2 162 . GLY . 26997 2 163 . ASP . 26997 2 164 . LYS . 26997 2 165 . ILE . 26997 2 166 . ALA . 26997 2 167 . ILE . 26997 2 168 . TRP . 26997 2 169 . THR . 26997 2 170 . THR . 26997 2 171 . GLU . 26997 2 172 . CYS . 26997 2 173 . GLU . 26997 2 174 . ASN . 26997 2 175 . ARG . 26997 2 176 . ASP . 26997 2 177 . ALA . 26997 2 178 . VAL . 26997 2 179 . THR . 26997 2 180 . HIS . 26997 2 181 . ILE . 26997 2 182 . GLY . 26997 2 183 . ARG . 26997 2 184 . VAL . 26997 2 185 . TYR . 26997 2 186 . LYS . 26997 2 187 . GLU . 26997 2 188 . ARG . 26997 2 189 . LEU . 26997 2 190 . GLY . 26997 2 191 . LEU . 26997 2 192 . PRO . 26997 2 193 . PRO . 26997 2 194 . LYS . 26997 2 195 . ILE . 26997 2 196 . VAL . 26997 2 197 . ILE . 26997 2 198 . GLY . 26997 2 199 . TYR . 26997 2 200 . GLN . 26997 2 201 . SER . 26997 2 202 . HIS . 26997 2 203 . ALA . 26997 2 204 . ASP . 26997 2 205 . THR . 26997 2 206 . ALA . 26997 2 207 . THR . 26997 2 208 . LYS . 26997 2 209 . SER . 26997 2 210 . GLY . 26997 2 211 . SER . 26997 2 212 . THR . 26997 2 213 . THR . 26997 2 214 . LYS . 26997 2 215 . ASN . 26997 2 216 . ARG . 26997 2 217 . PHE . 26997 2 218 . VAL . 26997 2 219 . VAL . 26997 2 stop_ loop_ _Entity_poly_seq.Hetero _Entity_poly_seq.Mon_ID _Entity_poly_seq.Num _Entity_poly_seq.Comp_index_ID _Entity_poly_seq.Entry_ID _Entity_poly_seq.Entity_ID . GLY 1 1 26997 2 . SER 2 2 26997 2 . MET 3 3 26997 2 . ALA 4 4 26997 2 . THR 5 5 26997 2 . VAL 6 6 26997 2 . GLU 7 7 26997 2 . PRO 8 8 26997 2 . GLU 9 9 26997 2 . THR 10 10 26997 2 . THR 11 11 26997 2 . PRO 12 12 26997 2 . THR 13 13 26997 2 . THR 14 14 26997 2 . ASN 15 15 26997 2 . PRO 16 16 26997 2 . PRO 17 17 26997 2 . PRO 18 18 26997 2 . ALA 19 19 26997 2 . GLU 20 20 26997 2 . GLU 21 21 26997 2 . GLU 22 22 26997 2 . LYS 23 23 26997 2 . THR 24 24 26997 2 . GLU 25 25 26997 2 . SER 26 26 26997 2 . ASN 27 27 26997 2 . GLN 28 28 26997 2 . GLU 29 29 26997 2 . VAL 30 30 26997 2 . ALA 31 31 26997 2 . ASN 32 32 26997 2 . PRO 33 33 26997 2 . GLU 34 34 26997 2 . HIS 35 35 26997 2 . TYR 36 36 26997 2 . ILE 37 37 26997 2 . LYS 38 38 26997 2 . HIS 39 39 26997 2 . PRO 40 40 26997 2 . LEU 41 41 26997 2 . GLN 42 42 26997 2 . ASN 43 43 26997 2 . ARG 44 44 26997 2 . TRP 45 45 26997 2 . ALA 46 46 26997 2 . LEU 47 47 26997 2 . TRP 48 48 26997 2 . PHE 49 49 26997 2 . PHE 50 50 26997 2 . LYS 51 51 26997 2 . ASN 52 52 26997 2 . ASP 53 53 26997 2 . LYS 54 54 26997 2 . SER 55 55 26997 2 . LYS 56 56 26997 2 . THR 57 57 26997 2 . TRP 58 58 26997 2 . GLN 59 59 26997 2 . ALA 60 60 26997 2 . ASN 61 61 26997 2 . LEU 62 62 26997 2 . ARG 63 63 26997 2 . LEU 64 64 26997 2 . ILE 65 65 26997 2 . SER 66 66 26997 2 . LYS 67 67 26997 2 . PHE 68 68 26997 2 . ASP 69 69 26997 2 . THR 70 70 26997 2 . VAL 71 71 26997 2 . GLU 72 72 26997 2 . ASP 73 73 26997 2 . PHE 74 74 26997 2 . TRP 75 75 26997 2 . ALA 76 76 26997 2 . LEU 77 77 26997 2 . TYR 78 78 26997 2 . ASN 79 79 26997 2 . HIS 80 80 26997 2 . ILE 81 81 26997 2 . GLN 82 82 26997 2 . LEU 83 83 26997 2 . SER 84 84 26997 2 . SER 85 85 26997 2 . ASN 86 86 26997 2 . LEU 87 87 26997 2 . MET 88 88 26997 2 . PRO 89 89 26997 2 . GLY 90 90 26997 2 . CYS 91 91 26997 2 . ASP 92 92 26997 2 . TYR 93 93 26997 2 . SER 94 94 26997 2 . LEU 95 95 26997 2 . PHE 96 96 26997 2 . LYS 97 97 26997 2 . ASP 98 98 26997 2 . GLY 99 99 26997 2 . ILE 100 100 26997 2 . GLU 101 101 26997 2 . PRO 102 102 26997 2 . MET 103 103 26997 2 . TRP 104 104 26997 2 . GLU 105 105 26997 2 . ASP 106 106 26997 2 . GLU 107 107 26997 2 . LYS 108 108 26997 2 . ASN 109 109 26997 2 . LYS 110 110 26997 2 . ARG 111 111 26997 2 . GLY 112 112 26997 2 . GLY 113 113 26997 2 . ARG 114 114 26997 2 . TRP 115 115 26997 2 . LEU 116 116 26997 2 . ILE 117 117 26997 2 . THR 118 118 26997 2 . LEU 119 119 26997 2 . ASN 120 120 26997 2 . LYS 121 121 26997 2 . GLN 122 122 26997 2 . GLN 123 123 26997 2 . ARG 124 124 26997 2 . ARG 125 125 26997 2 . SER 126 126 26997 2 . ASP 127 127 26997 2 . LEU 128 128 26997 2 . ASP 129 129 26997 2 . ARG 130 130 26997 2 . PHE 131 131 26997 2 . TRP 132 132 26997 2 . LEU 133 133 26997 2 . GLU 134 134 26997 2 . THR 135 135 26997 2 . LEU 136 136 26997 2 . LEU 137 137 26997 2 . CYS 138 138 26997 2 . LEU 139 139 26997 2 . ILE 140 140 26997 2 . GLY 141 141 26997 2 . GLU 142 142 26997 2 . SER 143 143 26997 2 . PHE 144 144 26997 2 . ASP 145 145 26997 2 . ASP 146 146 26997 2 . TYR 147 147 26997 2 . SER 148 148 26997 2 . ASP 149 149 26997 2 . ASP 150 150 26997 2 . VAL 151 151 26997 2 . CYS 152 152 26997 2 . GLY 153 153 26997 2 . ALA 154 154 26997 2 . VAL 155 155 26997 2 . VAL 156 156 26997 2 . ASN 157 157 26997 2 . VAL 158 158 26997 2 . ARG 159 159 26997 2 . ALA 160 160 26997 2 . LYS 161 161 26997 2 . GLY 162 162 26997 2 . ASP 163 163 26997 2 . LYS 164 164 26997 2 . ILE 165 165 26997 2 . ALA 166 166 26997 2 . ILE 167 167 26997 2 . TRP 168 168 26997 2 . THR 169 169 26997 2 . THR 170 170 26997 2 . GLU 171 171 26997 2 . CYS 172 172 26997 2 . GLU 173 173 26997 2 . ASN 174 174 26997 2 . ARG 175 175 26997 2 . ASP 176 176 26997 2 . ALA 177 177 26997 2 . VAL 178 178 26997 2 . THR 179 179 26997 2 . HIS 180 180 26997 2 . ILE 181 181 26997 2 . GLY 182 182 26997 2 . ARG 183 183 26997 2 . VAL 184 184 26997 2 . TYR 185 185 26997 2 . LYS 186 186 26997 2 . GLU 187 187 26997 2 . ARG 188 188 26997 2 . LEU 189 189 26997 2 . GLY 190 190 26997 2 . LEU 191 191 26997 2 . PRO 192 192 26997 2 . PRO 193 193 26997 2 . LYS 194 194 26997 2 . ILE 195 195 26997 2 . VAL 196 196 26997 2 . ILE 197 197 26997 2 . GLY 198 198 26997 2 . TYR 199 199 26997 2 . GLN 200 200 26997 2 . SER 201 201 26997 2 . HIS 202 202 26997 2 . ALA 203 203 26997 2 . ASP 204 204 26997 2 . THR 205 205 26997 2 . ALA 206 206 26997 2 . THR 207 207 26997 2 . LYS 208 208 26997 2 . SER 209 209 26997 2 . GLY 210 210 26997 2 . SER 211 211 26997 2 . THR 212 212 26997 2 . THR 213 213 26997 2 . LYS 214 214 26997 2 . ASN 215 215 26997 2 . ARG 216 216 26997 2 . PHE 217 217 26997 2 . VAL 218 218 26997 2 . VAL 219 219 26997 2 stop_ save_ #################### # Natural source # #################### save_natural_source _Entity_natural_src_list.Sf_category natural_source _Entity_natural_src_list.Sf_framecode natural_source _Entity_natural_src_list.Entry_ID 26997 _Entity_natural_src_list.ID 1 loop_ _Entity_natural_src.ID _Entity_natural_src.Entity_ID _Entity_natural_src.Entity_label _Entity_natural_src.Entity_chimera_segment_ID _Entity_natural_src.NCBI_taxonomy_ID _Entity_natural_src.Type _Entity_natural_src.Common _Entity_natural_src.Organism_name_scientific _Entity_natural_src.Organism_name_common _Entity_natural_src.Organism_acronym _Entity_natural_src.ICTVdb_decimal_code _Entity_natural_src.Superkingdom _Entity_natural_src.Kingdom _Entity_natural_src.Genus _Entity_natural_src.Species _Entity_natural_src.Strain _Entity_natural_src.Variant _Entity_natural_src.Organ _Entity_natural_src.Tissue _Entity_natural_src.Tissue_fraction _Entity_natural_src.Cell_line _Entity_natural_src.Cell_type _Entity_natural_src.ATCC_number _Entity_natural_src.Organelle _Entity_natural_src.Secretion _Entity_natural_src.Plasmid _Entity_natural_src.Gene_mnemonic _Entity_natural_src.Details _Entity_natural_src.Entry_ID _Entity_natural_src.Entity_natural_src_list_ID 1 1 $4E-BP1_44to87 . 9606 organism . 'Homo sapiens' Human . . Eukaryota Metazoa Homo sapiens . . . . . . . . . . . . . 26997 1 stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Entity_experimental_src_list.Sf_category experimental_source _Entity_experimental_src_list.Sf_framecode experimental_source _Entity_experimental_src_list.Entry_ID 26997 _Entity_experimental_src_list.ID 1 loop_ _Entity_experimental_src.ID _Entity_experimental_src.Entity_ID _Entity_experimental_src.Entity_label _Entity_experimental_src.Entity_chimera_segment_ID _Entity_experimental_src.Production_method _Entity_experimental_src.Host_org_scientific_name _Entity_experimental_src.Host_org_name_common _Entity_experimental_src.Host_org_details _Entity_experimental_src.Host_org_NCBI_taxonomy_ID _Entity_experimental_src.Host_org_genus _Entity_experimental_src.Host_org_species _Entity_experimental_src.Host_org_strain _Entity_experimental_src.Host_org_variant _Entity_experimental_src.Host_org_ATCC_number _Entity_experimental_src.Vector_type _Entity_experimental_src.PDBview_host_org_vector_name _Entity_experimental_src.PDBview_plasmid_name _Entity_experimental_src.Vector_name _Entity_experimental_src.Vector_details _Entity_experimental_src.Vendor_name _Entity_experimental_src.Details _Entity_experimental_src.Entry_ID _Entity_experimental_src.Entity_experimental_src_list_ID 1 1 $4E-BP1_44to87 . 'recombinant technology' 'Escherichia coli' . . . Escherichia coli 'Escherichia coli' . . . . . pET30 . . . 26997 1 stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Sample.Sf_category sample _Sample.Sf_framecode sample_1 _Sample.Entry_ID 26997 _Sample.ID 1 _Sample.Type solution _Sample.Sub_type . _Sample.Details . _Sample.Aggregate_sample_number . _Sample.Solvent_system '95% H2O/5% D2O' _Sample.Preparation_date . _Sample.Preparation_expiration_date . _Sample.Polycrystallization_protocol . _Sample.Single_crystal_protocol . _Sample.Crystal_grow_apparatus . _Sample.Crystal_grow_atmosphere . _Sample.Crystal_grow_details . _Sample.Crystal_grow_method . _Sample.Crystal_grow_method_cit_ID . _Sample.Crystal_grow_pH . _Sample.Crystal_grow_pH_range . _Sample.Crystal_grow_pressure . _Sample.Crystal_grow_pressure_esd . _Sample.Crystal_grow_seeding . _Sample.Crystal_grow_seeding_cit_ID . _Sample.Crystal_grow_temp . _Sample.Crystal_grow_temp_details . _Sample.Crystal_grow_temp_esd . _Sample.Crystal_grow_time . _Sample.Oriented_sample_prep_protocol . _Sample.Lyophilization_cryo_protectant . _Sample.Storage_protocol . loop_ _Sample_component.ID _Sample_component.Mol_common_name _Sample_component.Isotopic_labeling _Sample_component.Assembly_ID _Sample_component.Assembly_label _Sample_component.Entity_ID _Sample_component.Entity_label _Sample_component.Product_ID _Sample_component.Type _Sample_component.Concentration_val _Sample_component.Concentration_val_min _Sample_component.Concentration_val_max _Sample_component.Concentration_val_units _Sample_component.Concentration_val_err _Sample_component.Vendor _Sample_component.Vendor_product_name _Sample_component.Vendor_product_code _Sample_component.Entry_ID _Sample_component.Sample_ID 1 4E-BP1_44to87 '[U-13C; U-15N]' . . 1 $4E-BP1_44to87 . . 0.25 0.1 0.4 mM . . . . 26997 1 2 NaH2PO4/Na2HPO4 'natural abundance' . . . . . . 20 . . mM . . . . 26997 1 3 NaCl 'natural abundance' . . . . . . 50 . . mM . . . . 26997 1 4 DTT 'natural abundance' . . . . . . 2 . . mM . . . . 26997 1 stop_ save_ ####################### # Sample conditions # ####################### save_sample_conditions_1 _Sample_condition_list.Sf_category sample_conditions _Sample_condition_list.Sf_framecode sample_conditions_1 _Sample_condition_list.Entry_ID 26997 _Sample_condition_list.ID 1 _Sample_condition_list.Details '20 mM NaH2PO4/Na2HPO4, pH 6.5, 50 mM NaCl, 2 mM DTT, 5% D2O' loop_ _Sample_condition_variable.Type _Sample_condition_variable.Val _Sample_condition_variable.Val_err _Sample_condition_variable.Val_units _Sample_condition_variable.Entry_ID _Sample_condition_variable.Sample_condition_list_ID 'ionic strength' 0.070 . M 26997 1 pH 6.500 . pH 26997 1 pressure 1.000 . atm 26997 1 temperature 298.000 . K 26997 1 stop_ save_ ############################ # Computer software used # ############################ save_XEASY _Software.Sf_category software _Software.Sf_framecode XEASY _Software.Entry_ID 26997 _Software.ID 1 _Software.Type . _Software.Name XEASY _Software.Version 1.9.0 _Software.DOI . _Software.Details . loop_ _Vendor.Name _Vendor.Address _Vendor.Electronic_address _Vendor.Entry_ID _Vendor.Software_ID 'Keller and Wuthrich' . . 26997 1 stop_ loop_ _Task.Task _Task.Entry_ID _Task.Software_ID 'chemical shift assignment' 26997 1 stop_ save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_Bruker_750 _NMR_spectrometer.Sf_category NMR_spectrometer _NMR_spectrometer.Sf_framecode Bruker_750 _NMR_spectrometer.Entry_ID 26997 _NMR_spectrometer.ID 1 _NMR_spectrometer.Details . _NMR_spectrometer.Manufacturer Bruker _NMR_spectrometer.Model Avance _NMR_spectrometer.Serial_number . _NMR_spectrometer.Field_strength 750 save_ save_NMR_spectrometer_list _NMR_spectrometer_list.Sf_category NMR_spectrometer_list _NMR_spectrometer_list.Sf_framecode NMR_spectrometer_list _NMR_spectrometer_list.Entry_ID 26997 _NMR_spectrometer_list.ID 1 loop_ _NMR_spectrometer_view.ID _NMR_spectrometer_view.Name _NMR_spectrometer_view.Manufacturer _NMR_spectrometer_view.Model _NMR_spectrometer_view.Serial_number _NMR_spectrometer_view.Field_strength _NMR_spectrometer_view.Details _NMR_spectrometer_view.Citation_ID _NMR_spectrometer_view.Citation_label _NMR_spectrometer_view.Entry_ID _NMR_spectrometer_view.NMR_spectrometer_list_ID 1 Bruker_750 Bruker Avance . 750 . . . 26997 1 stop_ save_ ############################# # NMR applied experiments # ############################# save_experiment_list _Experiment_list.Sf_category experiment_list _Experiment_list.Sf_framecode experiment_list _Experiment_list.Entry_ID 26997 _Experiment_list.ID 1 _Experiment_list.Details . loop_ _Experiment.ID _Experiment.Name _Experiment.Raw_data_flag _Experiment.NMR_spec_expt_ID _Experiment.NMR_spec_expt_label _Experiment.MS_expt_ID _Experiment.MS_expt_label _Experiment.SAXS_expt_ID _Experiment.SAXS_expt_label _Experiment.FRET_expt_ID _Experiment.FRET_expt_label _Experiment.EMR_expt_ID _Experiment.EMR_expt_label _Experiment.Sample_ID _Experiment.Sample_label _Experiment.Sample_state _Experiment.Sample_volume _Experiment.Sample_volume_units _Experiment.Sample_condition_list_ID _Experiment.Sample_condition_list_label _Experiment.Sample_spinning_rate _Experiment.Sample_angle _Experiment.NMR_tube_type _Experiment.NMR_spectrometer_ID _Experiment.NMR_spectrometer_label _Experiment.NMR_spectrometer_probe_ID _Experiment.NMR_spectrometer_probe_label _Experiment.NMR_spectral_processing_ID _Experiment.NMR_spectral_processing_label _Experiment.Mass_spectrometer_ID _Experiment.Mass_spectrometer_label _Experiment.Xray_instrument_ID _Experiment.Xray_instrument_label _Experiment.Fluorescence_instrument_ID _Experiment.Fluorescence_instrument_label _Experiment.EMR_instrument_ID _Experiment.EMR_instrument_label _Experiment.Chromatographic_system_ID _Experiment.Chromatographic_system_label _Experiment.Chromatographic_column_ID _Experiment.Chromatographic_column_label _Experiment.Entry_ID _Experiment.Experiment_list_ID 1 HNCO no . . . . . . . . . . 1 $sample_1 isotropic . . 1 $sample_conditions_1 . . . 1 $Bruker_750 . . . . . . . . . . . . . . . . 26997 1 2 HNCA no . . . . . . . . . . 1 $sample_1 isotropic . . 1 $sample_conditions_1 . . . 1 $Bruker_750 . . . . . . . . . . . . . . . . 26997 1 3 HSQC no . . . . . . . . . . 1 $sample_1 isotropic . . 1 $sample_conditions_1 . . . 1 $Bruker_750 . . . . . . . . . . . . . . . . 26997 1 4 HNCACO no . . . . . . . . . . 1 $sample_1 isotropic . . 1 $sample_conditions_1 . . . 1 $Bruker_750 . . . . . . . . . . . . . . . . 26997 1 5 HNCOCA no . . . . . . . . . . 1 $sample_1 isotropic . . 1 $sample_conditions_1 . . . 1 $Bruker_750 . . . . . . . . . . . . . . . . 26997 1 stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chemical_shift_reference_1 _Chem_shift_reference.Sf_category chem_shift_reference _Chem_shift_reference.Sf_framecode chemical_shift_reference_1 _Chem_shift_reference.Entry_ID 26997 _Chem_shift_reference.ID 1 _Chem_shift_reference.Details . loop_ _Chem_shift_ref.Atom_type _Chem_shift_ref.Atom_isotope_number _Chem_shift_ref.Mol_common_name _Chem_shift_ref.Atom_group _Chem_shift_ref.Concentration_val _Chem_shift_ref.Concentration_units _Chem_shift_ref.Solvent _Chem_shift_ref.Rank _Chem_shift_ref.Chem_shift_units _Chem_shift_ref.Chem_shift_val _Chem_shift_ref.Ref_method _Chem_shift_ref.Ref_type _Chem_shift_ref.Indirect_shift_ratio _Chem_shift_ref.External_ref_loc _Chem_shift_ref.External_ref_sample_geometry _Chem_shift_ref.External_ref_axis _Chem_shift_ref.Ref_correction_type _Chem_shift_ref.Correction_val _Chem_shift_ref.Entry_ID _Chem_shift_ref.Chem_shift_reference_ID C 13 DSS 'methyl protons' . . . . ppm 0.00 na indirect 0.251449530 . . . . . 26997 1 H 1 DSS 'methyl protons' . . . . ppm 0.00 internal direct 1.000000000 . . . . . 26997 1 N 15 DSS 'methyl protons' . . . . ppm 0.00 na indirect 0.101329118 . . . . . 26997 1 stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_assigned_chem_shift_list _Assigned_chem_shift_list.Sf_category assigned_chemical_shifts _Assigned_chem_shift_list.Sf_framecode assigned_chem_shift_list _Assigned_chem_shift_list.Entry_ID 26997 _Assigned_chem_shift_list.ID 1 _Assigned_chem_shift_list.Sample_condition_list_ID 1 _Assigned_chem_shift_list.Sample_condition_list_label $sample_conditions_1 _Assigned_chem_shift_list.Chem_shift_reference_ID 1 _Assigned_chem_shift_list.Chem_shift_reference_label $chemical_shift_reference_1 _Assigned_chem_shift_list.Chem_shift_1H_err . _Assigned_chem_shift_list.Chem_shift_13C_err . _Assigned_chem_shift_list.Chem_shift_15N_err . _Assigned_chem_shift_list.Chem_shift_31P_err . _Assigned_chem_shift_list.Chem_shift_2H_err . _Assigned_chem_shift_list.Chem_shift_19F_err . _Assigned_chem_shift_list.Error_derivation_method . _Assigned_chem_shift_list.Details ; Origin xeasy file /nmr/data/Leger/4E-BP_assignment_deposition/complex_with_m4E/chemical_shifts.prot ; _Assigned_chem_shift_list.Text_data_format . _Assigned_chem_shift_list.Text_data . loop_ _Chem_shift_experiment.Experiment_ID _Chem_shift_experiment.Experiment_name _Chem_shift_experiment.Sample_ID _Chem_shift_experiment.Sample_label _Chem_shift_experiment.Sample_state _Chem_shift_experiment.Entry_ID _Chem_shift_experiment.Assigned_chem_shift_list_ID 1 HNCO 1 $sample_1 isotropic 26997 1 2 HNCA 1 $sample_1 isotropic 26997 1 3 HSQC 1 $sample_1 isotropic 26997 1 4 HNCACO 1 $sample_1 isotropic 26997 1 5 HNCOCA 1 $sample_1 isotropic 26997 1 stop_ loop_ _Chem_shift_software.Software_ID _Chem_shift_software.Software_label _Chem_shift_software.Method_ID _Chem_shift_software.Method_label _Chem_shift_software.Entry_ID _Chem_shift_software.Assigned_chem_shift_list_ID 1 $XEASY . . 26997 1 stop_ loop_ _Atom_chem_shift.ID _Atom_chem_shift.Assembly_atom_ID _Atom_chem_shift.Entity_assembly_ID _Atom_chem_shift.Entity_ID _Atom_chem_shift.Comp_index_ID _Atom_chem_shift.Seq_ID _Atom_chem_shift.Comp_ID _Atom_chem_shift.Atom_ID _Atom_chem_shift.Atom_type _Atom_chem_shift.Atom_isotope_number _Atom_chem_shift.Val _Atom_chem_shift.Val_err _Atom_chem_shift.Assign_fig_of_merit _Atom_chem_shift.Ambiguity_code _Atom_chem_shift.Ambiguity_set_ID _Atom_chem_shift.Occupancy _Atom_chem_shift.Resonance_ID _Atom_chem_shift.Auth_entity_assembly_ID _Atom_chem_shift.Auth_asym_ID _Atom_chem_shift.Auth_seq_ID _Atom_chem_shift.Auth_comp_ID _Atom_chem_shift.Auth_atom_ID _Atom_chem_shift.Details _Atom_chem_shift.Entry_ID _Atom_chem_shift.Assigned_chem_shift_list_ID 1 . 1 1 4 4 SER H H 1 8.132 . . 1 . . 58 . . 44 SER H . 26997 1 2 . 1 1 4 4 SER C C 13 174.374 . . 1 . . 60 . . 44 SER C . 26997 1 3 . 1 1 4 4 SER CA C 13 57.657 . . 1 . . 127 . . 44 SER CA . 26997 1 4 . 1 1 4 4 SER N N 15 117.173 . . 1 . . 59 . . 44 SER N . 26997 1 5 . 1 1 5 5 THR H H 1 8.156 . . 1 . . 37 . . 45 THR H . 26997 1 6 . 1 1 5 5 THR C C 13 174.450 . . 1 . . 39 . . 45 THR C . 26997 1 7 . 1 1 5 5 THR CA C 13 61.285 . . 1 . . 121 . . 45 THR CA . 26997 1 8 . 1 1 5 5 THR N N 15 115.523 . . 1 . . 38 . . 45 THR N . 26997 1 9 . 1 1 6 6 THR H H 1 8.139 . . 1 . . 52 . . 46 THR H . 26997 1 10 . 1 1 6 6 THR C C 13 172.934 . . 1 . . 54 . . 46 THR C . 26997 1 11 . 1 1 6 6 THR CA C 13 59.411 . . 1 . . 125 . . 46 THR CA . 26997 1 12 . 1 1 6 6 THR N N 15 118.613 . . 1 . . 53 . . 46 THR N . 26997 1 13 . 1 1 8 8 GLY H H 1 8.435 . . 1 . . 97 . . 48 GLY H . 26997 1 14 . 1 1 8 8 GLY C C 13 174.747 . . 1 . . 99 . . 48 GLY C . 26997 1 15 . 1 1 8 8 GLY CA C 13 44.880 . . 1 . . 144 . . 48 GLY CA . 26997 1 16 . 1 1 8 8 GLY N N 15 109.550 . . 1 . . 98 . . 48 GLY N . 26997 1 17 . 1 1 9 9 GLY H H 1 8.173 . . 1 . . 106 . . 49 GLY H . 26997 1 18 . 1 1 9 9 GLY C C 13 174.219 . . 1 . . 108 . . 49 GLY C . 26997 1 19 . 1 1 9 9 GLY CA C 13 44.776 . . 1 . . 142 . . 49 GLY CA . 26997 1 20 . 1 1 9 9 GLY N N 15 108.804 . . 1 . . 107 . . 49 GLY N . 26997 1 21 . 1 1 10 10 THR H H 1 7.977 . . 1 . . 43 . . 50 THR H . 26997 1 22 . 1 1 10 10 THR C C 13 173.995 . . 1 . . 45 . . 50 THR C . 26997 1 23 . 1 1 10 10 THR CA C 13 61.281 . . 1 . . 122 . . 50 THR CA . 26997 1 24 . 1 1 10 10 THR N N 15 113.819 . . 1 . . 44 . . 50 THR N . 26997 1 25 . 1 1 11 11 ARG H H 1 8.106 . . 1 . . 16 . . 51 ARG H . 26997 1 26 . 1 1 11 11 ARG C C 13 175.723 . . 1 . . 18 . . 51 ARG C . 26997 1 27 . 1 1 11 11 ARG CA C 13 55.429 . . 1 . . 114 . . 51 ARG CA . 26997 1 28 . 1 1 11 11 ARG N N 15 124.666 . . 1 . . 17 . . 51 ARG N . 26997 1 29 . 1 1 12 12 ILE H H 1 8.376 . . 1 . . 13 . . 52 ILE H . 26997 1 30 . 1 1 12 12 ILE C C 13 173.779 . . 1 . . 15 . . 52 ILE C . 26997 1 31 . 1 1 12 12 ILE CA C 13 61.881 . . 1 . . 113 . . 52 ILE CA . 26997 1 32 . 1 1 12 12 ILE N N 15 127.129 . . 1 . . 14 . . 52 ILE N . 26997 1 33 . 1 1 13 13 ILE H H 1 7.226 . . 1 . . 10 . . 53 ILE H . 26997 1 34 . 1 1 13 13 ILE C C 13 176.023 . . 1 . . 12 . . 53 ILE C . 26997 1 35 . 1 1 13 13 ILE CA C 13 58.498 . . 1 . . 112 . . 53 ILE CA . 26997 1 36 . 1 1 13 13 ILE N N 15 127.448 . . 1 . . 11 . . 53 ILE N . 26997 1 37 . 1 1 14 14 TYR H H 1 8.444 . . 1 . . 28 . . 54 TYR H . 26997 1 38 . 1 1 14 14 TYR C C 13 175.449 . . 1 . . 30 . . 54 TYR C . 26997 1 39 . 1 1 14 14 TYR CA C 13 55.413 . . 1 . . 118 . . 54 TYR CA . 26997 1 40 . 1 1 14 14 TYR N N 15 121.170 . . 1 . . 29 . . 54 TYR N . 26997 1 41 . 1 1 15 15 ASP H H 1 8.689 . . 1 . . 19 . . 55 ASP H . 26997 1 42 . 1 1 15 15 ASP C C 13 175.346 . . 1 . . 21 . . 55 ASP C . 26997 1 43 . 1 1 15 15 ASP CA C 13 52.143 . . 1 . . 115 . . 55 ASP CA . 26997 1 44 . 1 1 15 15 ASP N N 15 122.423 . . 1 . . 20 . . 55 ASP N . 26997 1 45 . 1 1 16 16 ARG H H 1 8.637 . . 1 . . 25 . . 56 ARG H . 26997 1 46 . 1 1 16 16 ARG C C 13 177.304 . . 1 . . 27 . . 56 ARG C . 26997 1 47 . 1 1 16 16 ARG CA C 13 59.760 . . 1 . . 117 . . 56 ARG CA . 26997 1 48 . 1 1 16 16 ARG N N 15 121.205 . . 1 . . 26 . . 56 ARG N . 26997 1 49 . 1 1 17 17 LYS H H 1 8.143 . . 1 . . 55 . . 57 LYS H . 26997 1 50 . 1 1 17 17 LYS C C 13 178.886 . . 1 . . 57 . . 57 LYS C . 26997 1 51 . 1 1 17 17 LYS CA C 13 59.414 . . 1 . . 126 . . 57 LYS CA . 26997 1 52 . 1 1 17 17 LYS N N 15 118.243 . . 1 . . 56 . . 57 LYS N . 26997 1 53 . 1 1 18 18 PHE H H 1 7.959 . . 1 . . 34 . . 58 PHE H . 26997 1 54 . 1 1 18 18 PHE C C 13 178.434 . . 1 . . 36 . . 58 PHE C . 26997 1 55 . 1 1 18 18 PHE CA C 13 60.935 . . 1 . . 120 . . 58 PHE CA . 26997 1 56 . 1 1 18 18 PHE N N 15 120.453 . . 1 . . 35 . . 58 PHE N . 26997 1 57 . 1 1 19 19 LEU H H 1 7.930 . . 1 . . 91 . . 59 LEU H . 26997 1 58 . 1 1 19 19 LEU C C 13 180.458 . . 1 . . 93 . . 59 LEU C . 26997 1 59 . 1 1 19 19 LEU CA C 13 58.333 . . 1 . . 138 . . 59 LEU CA . 26997 1 60 . 1 1 19 19 LEU N N 15 116.784 . . 1 . . 92 . . 59 LEU N . 26997 1 61 . 1 1 20 20 MET H H 1 8.395 . . 1 . . 85 . . 60 MET H . 26997 1 62 . 1 1 20 20 MET C C 13 179.925 . . 1 . . 87 . . 60 MET C . 26997 1 63 . 1 1 20 20 MET CA C 13 56.954 . . 1 . . 137 . . 60 MET CA . 26997 1 64 . 1 1 20 20 MET N N 15 116.193 . . 1 . . 86 . . 60 MET N . 26997 1 65 . 1 1 21 21 GLU H H 1 7.993 . . 1 . . 31 . . 61 GLU H . 26997 1 66 . 1 1 21 21 GLU C C 13 179.330 . . 1 . . 33 . . 61 GLU C . 26997 1 67 . 1 1 21 21 GLU CA C 13 58.470 . . 1 . . 119 . . 61 GLU CA . 26997 1 68 . 1 1 21 21 GLU N N 15 122.503 . . 1 . . 32 . . 61 GLU N . 26997 1 69 . 1 1 22 22 CYS H H 1 7.121 . . 1 . . 82 . . 62 CYS H . 26997 1 70 . 1 1 22 22 CYS C C 13 174.752 . . 1 . . 84 . . 62 CYS C . 26997 1 71 . 1 1 22 22 CYS CA C 13 61.286 . . 1 . . 135 . . 62 CYS CA . 26997 1 72 . 1 1 22 22 CYS N N 15 114.808 . . 1 . . 83 . . 62 CYS N . 26997 1 73 . 1 1 23 23 ARG H H 1 6.697 . . 1 . . 4 . . 63 ARG H . 26997 1 74 . 1 1 23 23 ARG C C 13 175.189 . . 1 . . 6 . . 63 ARG C . 26997 1 75 . 1 1 23 23 ARG CA C 13 57.782 . . 1 . . 109 . . 63 ARG CA . 26997 1 76 . 1 1 23 23 ARG N N 15 118.477 . . 1 . . 5 . . 63 ARG N . 26997 1 77 . 1 1 24 24 ASN H H 1 7.002 . . 1 . . 94 . . 64 ASN H . 26997 1 78 . 1 1 24 24 ASN C C 13 174.896 . . 1 . . 96 . . 64 ASN C . 26997 1 79 . 1 1 24 24 ASN CA C 13 52.494 . . 1 . . 139 . . 64 ASN CA . 26997 1 80 . 1 1 24 24 ASN N N 15 112.134 . . 1 . . 95 . . 64 ASN N . 26997 1 81 . 1 1 25 25 SER H H 1 7.078 . . 1 . . 76 . . 65 SER H . 26997 1 82 . 1 1 25 25 SER C C 13 175.208 . . 1 . . 78 . . 65 SER C . 26997 1 83 . 1 1 25 25 SER CA C 13 55.778 . . 1 . . 133 . . 65 SER CA . 26997 1 84 . 1 1 25 25 SER N N 15 115.617 . . 1 . . 77 . . 65 SER N . 26997 1 85 . 1 1 27 27 VAL H H 1 7.405 . . 1 . . 79 . . 67 VAL H . 26997 1 86 . 1 1 27 27 VAL C C 13 176.959 . . 1 . . 81 . . 67 VAL C . 26997 1 87 . 1 1 27 27 VAL CA C 13 63.522 . . 1 . . 134 . . 67 VAL CA . 26997 1 88 . 1 1 27 27 VAL N N 15 115.034 . . 1 . . 80 . . 67 VAL N . 26997 1 89 . 1 1 28 28 THR H H 1 7.675 . . 1 . . 103 . . 68 THR H . 26997 1 90 . 1 1 28 28 THR C C 13 174.929 . . 1 . . 105 . . 68 THR C . 26997 1 91 . 1 1 28 28 THR CA C 13 63.038 . . 1 . . 141 . . 68 THR CA . 26997 1 92 . 1 1 28 28 THR N N 15 107.942 . . 1 . . 104 . . 68 THR N . 26997 1 93 . 1 1 29 29 LYS H H 1 7.090 . . 1 . . 1 . . 69 LYS H . 26997 1 94 . 1 1 29 29 LYS C C 13 175.900 . . 1 . . 3 . . 69 LYS C . 26997 1 95 . 1 1 29 29 LYS CA C 13 55.780 . . 1 . . 110 . . 69 LYS CA . 26997 1 96 . 1 1 29 29 LYS N N 15 118.083 . . 1 . . 2 . . 69 LYS N . 26997 1 97 . 1 1 30 30 THR H H 1 7.198 . . 1 . . 73 . . 70 THR H . 26997 1 98 . 1 1 30 30 THR C C 13 171.230 . . 1 . . 75 . . 70 THR C . 26997 1 99 . 1 1 30 30 THR CA C 13 58.619 . . 1 . . 132 . . 70 THR CA . 26997 1 100 . 1 1 30 30 THR N N 15 115.929 . . 1 . . 74 . . 70 THR N . 26997 1 101 . 1 1 33 33 ARG H H 1 8.485 . . 1 . . 22 . . 73 ARG H . 26997 1 102 . 1 1 33 33 ARG C C 13 176.561 . . 1 . . 24 . . 73 ARG C . 26997 1 103 . 1 1 33 33 ARG CA C 13 56.857 . . 1 . . 116 . . 73 ARG CA . 26997 1 104 . 1 1 33 33 ARG N N 15 122.635 . . 1 . . 23 . . 73 ARG N . 26997 1 105 . 1 1 34 34 ASP H H 1 8.417 . . 1 . . 88 . . 74 ASP H . 26997 1 106 . 1 1 34 34 ASP C C 13 175.499 . . 1 . . 90 . . 74 ASP C . 26997 1 107 . 1 1 34 34 ASP CA C 13 53.549 . . 1 . . 136 . . 74 ASP CA . 26997 1 108 . 1 1 34 34 ASP N N 15 116.634 . . 1 . . 89 . . 74 ASP N . 26997 1 109 . 1 1 35 35 LEU H H 1 7.266 . . 1 . . 7 . . 75 LEU H . 26997 1 110 . 1 1 35 35 LEU C C 13 175.275 . . 1 . . 9 . . 75 LEU C . 26997 1 111 . 1 1 35 35 LEU CA C 13 52.968 . . 1 . . 111 . . 75 LEU CA . 26997 1 112 . 1 1 35 35 LEU N N 15 121.449 . . 1 . . 8 . . 75 LEU N . 26997 1 113 . 1 1 37 37 THR H H 1 8.690 . . 1 . . 46 . . 77 THR H . 26997 1 114 . 1 1 37 37 THR C C 13 173.714 . . 1 . . 48 . . 77 THR C . 26997 1 115 . 1 1 37 37 THR CA C 13 61.990 . . 1 . . 123 . . 77 THR CA . 26997 1 116 . 1 1 37 37 THR N N 15 117.842 . . 1 . . 47 . . 77 THR N . 26997 1 117 . 1 1 38 38 ILE H H 1 7.956 . . 1 . . 67 . . 78 ILE H . 26997 1 118 . 1 1 38 38 ILE C C 13 173.028 . . 1 . . 69 . . 78 ILE C . 26997 1 119 . 1 1 38 38 ILE CA C 13 57.539 . . 1 . . 130 . . 78 ILE CA . 26997 1 120 . 1 1 38 38 ILE N N 15 127.848 . . 1 . . 68 . . 78 ILE N . 26997 1 121 . 1 1 40 40 GLY H H 1 8.416 . . 1 . . 40 . . 80 GLY H . 26997 1 122 . 1 1 40 40 GLY C C 13 173.050 . . 1 . . 42 . . 80 GLY C . 26997 1 123 . 1 1 40 40 GLY CA C 13 44.998 . . 1 . . 143 . . 80 GLY CA . 26997 1 124 . 1 1 40 40 GLY N N 15 112.897 . . 1 . . 41 . . 80 GLY N . 26997 1 125 . 1 1 41 41 VAL H H 1 7.983 . . 1 . . 61 . . 81 VAL H . 26997 1 126 . 1 1 41 41 VAL C C 13 175.952 . . 1 . . 63 . . 81 VAL C . 26997 1 127 . 1 1 41 41 VAL CA C 13 63.869 . . 1 . . 128 . . 81 VAL CA . 26997 1 128 . 1 1 41 41 VAL N N 15 117.822 . . 1 . . 62 . . 81 VAL N . 26997 1 129 . 1 1 42 42 THR H H 1 8.326 . . 1 . . 100 . . 82 THR H . 26997 1 130 . 1 1 42 42 THR C C 13 173.557 . . 1 . . 102 . . 82 THR C . 26997 1 131 . 1 1 42 42 THR CA C 13 60.103 . . 1 . . 140 . . 82 THR CA . 26997 1 132 . 1 1 42 42 THR N N 15 106.390 . . 1 . . 101 . . 82 THR N . 26997 1 133 . 1 1 43 43 SER H H 1 7.742 . . 1 . . 64 . . 83 SER H . 26997 1 134 . 1 1 43 43 SER C C 13 172.051 . . 1 . . 66 . . 83 SER C . 26997 1 135 . 1 1 43 43 SER CA C 13 56.010 . . 1 . . 129 . . 83 SER CA . 26997 1 136 . 1 1 43 43 SER N N 15 117.005 . . 1 . . 65 . . 83 SER N . 26997 1 137 . 1 1 45 45 SER H H 1 8.315 . . 1 . . 49 . . 85 SER H . 26997 1 138 . 1 1 45 45 SER C C 13 173.321 . . 1 . . 51 . . 85 SER C . 26997 1 139 . 1 1 45 45 SER CA C 13 57.656 . . 1 . . 124 . . 85 SER CA . 26997 1 140 . 1 1 45 45 SER N N 15 118.010 . . 1 . . 50 . . 85 SER N . 26997 1 141 . 1 1 46 46 SER H H 1 7.913 . . 1 . . 70 . . 86 SER H . 26997 1 142 . 1 1 46 46 SER C C 13 180.824 . . 1 . . 72 . . 86 SER C . 26997 1 143 . 1 1 46 46 SER CA C 13 55.661 . . 1 . . 131 . . 86 SER CA . 26997 1 144 . 1 1 46 46 SER N N 15 127.376 . . 1 . . 71 . . 86 SER N . 26997 1 stop_ save_