BMRB Entry 25344

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR25344

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Title:
Calcium bound calmodulin C-terminal domain, E140Q mutant
Deposition date:
2014-11-14
Original release date:
2014-12-16
Authors:
Lundstrom, Patrik
Citation:

Citation: Niklasson, Markus; Ahlner, Alexandra; Andresen, Cecilia; Marsh, Joseph; Lundstrom, Patrik. "Fast and accurate resonance assignment of small-to-large proteins by combining automated and manual approaches"  PLOS Comput. Biol. 11, e1004022-e1004022 (2015).
PubMed: 25569628

Assembly members:

Assembly members:
CaM_E140Q_Tr2C, polymer, 73 residues, Formula weight is not available
CALCIUM ION, non-polymer, 40.078 Da.

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):

Entity Sequences (FASTA):
CaM_E140Q_Tr2C: MKDTDSEEEIREAFRVFDKD GNGYISAAELRHVMTNLGEK LTDEEVDEMIREADIDGDGQ VNYEQFVQMMTAK

Data sets:
Data typeCount
13C chemical shifts305
15N chemical shifts71
1H chemical shifts473

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1subunit 11
2calcium ion2

Entities:

Entity 1, subunit 1 73 residues - Formula weight is not available

1   METLYSASPTHRASPSERGLUGLUGLUILE
2   ARGGLUALAPHEARGVALPHEASPLYSASP
3   GLYASNGLYTYRILESERALAALAGLULEU
4   ARGHISVALMETTHRASNLEUGLYGLULYS
5   LEUTHRASPGLUGLUVALASPGLUMETILE
6   ARGGLUALAASPILEASPGLYASPGLYGLN
7   VALASNTYRGLUGLNPHEVALGLNMETMET
8   THRALALYS

Entity 2, calcium ion - Ca - 40.078 Da.

1   CA

Related Database Links:

BMRB 11440 16994 17881 17981 17982 17983 18084 18323 19376
PDB 1CMF 1CMG 1F71 1FW4 1J7P 1YRT 1YRU 1ZOT 2COL 2HF5 2K3S 2KUH 2KZ2 2LLQ 2LQP 2RRT 4BYA 4RJD
DBJ BAC39089 BAF45809 BAI66109 BAI66110 BAI66111
EMBL CAB51566
GB AAH10730 AAO17827 AAQ14324 AAX61134 AAY87961
REF XP_001687795 XP_001869425 XP_007442524 XP_010190430 XP_011906627

Download HSQC peak lists in one of the following formats:
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