BMRB Entry 17983

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR17983

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Title:
Dynamics of isolated C domain of calmodulin complexed with PEP-19 in the presence of Ca2+
Deposition date:
2011-10-07
Original release date:
2011-10-10
Authors:
Wang, Xu; Kleerekoper, Quinn; Putkey, John
Citation:

Citation: Wang, Xu; Kleerekoper, Quinn; Xiong, Liang-wen; Putkey, John. "Intrinsic disorder of PEP-19 confers unique dynamic properties to apo and calcium calmodulin"  Biochemistry 49, 10287-10297 (2010).
PubMed: 20973509

Assembly members:

Assembly members:
isolcated_c_domain_of_calmodulin, polymer, 71 residues, Formula weight is not available
PEP-19, polymer, 62 residues, Formula weight is not available
CA, non-polymer, 40.078 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pLysS

Entity Sequences (FASTA):

Entity Sequences (FASTA):
isolcated_c_domain_of_calmodulin: DTDSEEEIREAFRVFDKDGN GYISAAELRHVMTNLGEKLT DEEVDEMIREADIDGDGQVN YEEFVQMMTAK
PEP-19: MAERQGAGATNGKDKTSGEN DGQKKVQEEFDIDMDAPETE RAAVAIQSQFRKFQKKKAGS QS

Data sets:
Data typeCount
15N chemical shifts65
1H chemical shifts65
heteronuclear NOE values63
T1 relaxation values63
T2 relaxation values64

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1isolcated_c_domain_of_calmodulin1
2Purkinje_cell_protein_42
3Calcium3

Entities:

Entity 1, isolcated_c_domain_of_calmodulin 71 residues - Formula weight is not available

1   ASPTHRASPSERGLUGLUGLUILEARGGLU
2   ALAPHEARGVALPHEASPLYSASPGLYASN
3   GLYTYRILESERALAALAGLULEUARGHIS
4   VALMETTHRASNLEUGLYGLULYSLEUTHR
5   ASPGLUGLUVALASPGLUMETILEARGGLU
6   ALAASPILEASPGLYASPGLYGLNVALASN
7   TYRGLUGLUPHEVALGLNMETMETTHRALA
8   LYS

Entity 2, Purkinje_cell_protein_4 62 residues - Formula weight is not available

1   METALAGLUARGGLNGLYALAGLYALATHR
2   ASNGLYLYSASPLYSTHRSERGLYGLUASN
3   ASPGLYGLNLYSLYSVALGLNGLUGLUPHE
4   ASPILEASPMETASPALAPROGLUTHRGLU
5   ARGALAALAVALALAILEGLNSERGLNPHE
6   ARGLYSPHEGLNLYSLYSLYSALAGLYSER
7   GLNSER

Entity 3, Calcium - Ca - 40.078 Da.

1   CA

Samples:

Ca2_C-CaM_PEP-19: isolcated c domain of calmodulin 0.5 mM; PEP-19 0.8 mM; calcium 5 mM; potassium chloride 100 mM; H2O 95%; D2O 5%

sample_conditions_1: ionic strength: 0.1 M; pH: 6.3; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
T1Ca2_C-CaM_PEP-19isotropicsample_conditions_1
T2Ca2_C-CaM_PEP-19isotropicsample_conditions_1
NOECa2_C-CaM_PEP-19isotropicsample_conditions_1
2D 1H-15N HSQCCa2_C-CaM_PEP-19isotropicsample_conditions_1
3D HNCACa2_C-CaM_PEP-19isotropicsample_conditions_1
3D HN(CO)CACa2_C-CaM_PEP-19isotropicsample_conditions_1
3D CBCA(CO)NHCa2_C-CaM_PEP-19isotropicsample_conditions_1
3D HNCACBCa2_C-CaM_PEP-19isotropicsample_conditions_1
3D HNCOCa2_C-CaM_PEP-19isotropicsample_conditions_1
3D 1H-15N NOESYCa2_C-CaM_PEP-19isotropicsample_conditions_1
3D 1H-15N TOCSYCa2_C-CaM_PEP-19isotropicsample_conditions_1

Software:

FELIX v2004, Accelrys Software Inc. - peak picking, processing

NMR spectrometers:

  • Bruker DPX 600 MHz

Related Database Links:

BMRB 11440 16994 17881 17981 17982 18084 18323 19376 25344 17981
PDB
DBJ BAC39089 BAF45809 BAI66109 BAI66110 BAI66111 BAF82653
EMBL CAB51566 CAB90443 CAD38515 CAG29315 CAJ70630
GB AAH10730 AAO17827 AAQ14324 AAX61134 AAY87961 AAB09033 AAC00024 AAC23994 AAH13791 AAL16810
REF XP_001687795 XP_001869425 XP_007442524 XP_009577260 XP_010190430 NP_001257467 NP_006189 XP_002830736 XP_002929216 XP_003280305
SP P48539
AlphaFold P48539

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks