BMRB Entry 17069
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR17069
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Citation: Schlenker, Casey; Goel, Anupam; Tripet, Brian; Menon, Smita; Lawrence, C. Martin; Valerie, Copie. "Structure and dynamics characterization of the E73 protein from Sulfolobus Spindle-shaped Virus Ragged Hills (SSV-RH), a hyperthermophilic crenarchaeal virus from Yellowstone National Park." Biochemistry ., .-..
Assembly members:
E73, polymer, 80 residues, 9578.2 Da.
Natural source: Common Name: Sulfolobus spindle Virus Taxonomy ID: 256994 Superkingdom: Virus Kingdom: not available Genus/species: Fusellovirus Sulfolobus spindle-shaped Virus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET14
Entity Sequences (FASTA):
E73: MHHHHHHMVESKKIAKKKTT
LAFDEDVYHTLKLVSVYLNR
DMTEIIEEAVVMWLIQNKEK
LPNELKPKIDEISKRFFPAK
- heteronucl_NOEs
- heteronucl_T1_relaxation
- heteronucl_T2_relaxation
- order_parameters
- spectral_density_values
| Data type | Count |
| heteronuclear NOE values | 58 |
| order parameters | 57 |
| spectral density values | 58 |
| T1 relaxation values | 58 |
| T2 relaxation values | 58 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | E73, chain 1 | 1 |
| 2 | E73, chain 2 | 1 |
Entities:
Entity 1, E73, chain 1 80 residues - 9578.2 Da.
| 1 | MET | HIS | HIS | HIS | HIS | HIS | HIS | MET | VAL | GLU | |
| 2 | SER | LYS | LYS | ILE | ALA | LYS | LYS | LYS | THR | THR | |
| 3 | LEU | ALA | PHE | ASP | GLU | ASP | VAL | TYR | HIS | THR | |
| 4 | LEU | LYS | LEU | VAL | SER | VAL | TYR | LEU | ASN | ARG | |
| 5 | ASP | MET | THR | GLU | ILE | ILE | GLU | GLU | ALA | VAL | |
| 6 | VAL | MET | TRP | LEU | ILE | GLN | ASN | LYS | GLU | LYS | |
| 7 | LEU | PRO | ASN | GLU | LEU | LYS | PRO | LYS | ILE | ASP | |
| 8 | GLU | ILE | SER | LYS | ARG | PHE | PHE | PRO | ALA | LYS |
Samples:
sample_relax: E73, [U-100% 13C; U-100% 15N], 5 mM; D2O 10%; H2O 90%; PMSF 0.1 mM; sodium azide 0.01%; potassium phosphate 50 mM; EDTA 1 mM
sample_conditions_1: ionic strength: 0.005 M; pH: 5.0; pressure: 1 atm; temperature: 312 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 15N T1 | sample_relax | anisotropic | sample_conditions_1 |
| 2D 15N T2 interleaved | sample_relax | anisotropic | sample_conditions_1 |
| 2D 15N {1H} nOe | sample_relax | anisotropic | sample_conditions_1 |
Software:
TOPSPIN, Bruker Biospin - collection
NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - data analysis, processing
SPARKY, Goddard - chemical shift assignment, data analysis, peak picking
ModelFree, Palmer - data analysis, geometry optimization, refinement
NMR spectrometers:
- Bruker DRX 600 MHz