BMRB Entry 16177

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16177

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Title:
Backbone and sidechain chemical shift assignments for E73 from SSV-RH
Deposition date:
2009-02-18
Original release date:
2009-08-10
Authors:
Schlenker, Casey; Menon, Smita; Lawrence, C. Martin; Copie, Valerie
Citation:

Citation: Schlenker, Casey; Menon, Smita; Lawrence, C. Martin; Copie, Valerie. "(1)H, (13)C, (15)N backbone and side chain NMR resonance assignments for E73 from Sulfolobus spindle-shaped virus ragged hills, a hyperthermophilic crenarchaeal virus from Yellowstone National Park."  Biomol. NMR Assignments 3, 219-222 (2009).
PubMed: 19888695

Assembly members:

Assembly members:
E73, polymer, 80 residues, 9578.2 Da.

Natural source:

Natural source:   Common Name: Sulfolobus spindle Virus   Taxonomy ID: 256994   Superkingdom: Virus   Kingdom: not available   Genus/species: not available Sulfolobus spindle-shaped Virus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET14

Entity Sequences (FASTA):

Entity Sequences (FASTA):
E73: MHHHHHHMVESKKIAKKKTT LAFDEDVYHTLKLVSVYLNR DMTEIIEEAVVMWLIQNKEK LPNELKPKIDEISKRFFPAK

Data sets:
Data typeCount
13C chemical shifts227
15N chemical shifts69
1H chemical shifts464

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1E73, chain 11
2E73, chain 21

Entities:

Entity 1, E73, chain 1 80 residues - 9578.2 Da.

Residues 1-7 are a six His tag and Met residue added during cloning to assist with purification. Sequence numbering for the purpose of assignment begins with the second Met residue.

1   METHISHISHISHISHISHISMETVALGLU
2   SERLYSLYSILEALALYSLYSLYSTHRTHR
3   LEUALAPHEASPGLUASPVALTYRHISTHR
4   LEULYSLEUVALSERVALTYRLEUASNARG
5   ASPMETTHRGLUILEILEGLUGLUALAVAL
6   VALMETTRPLEUILEGLNASNLYSGLULYS
7   LEUPROASNGLULEULYSPROLYSILEASP
8   GLUILESERLYSARGPHEPHEPROALALYS

Samples:

E73_sample_1: E73, [U-100% 13C; U-100% 15N], 15 mM; PMSF 1 mM; sodium azide 0.01%; potassium phosphate 50 mM; D2O 10%; EDTA 1 mM

E73_sample_conditions_1: ionic strength: 0.05 M; pH: 5.0; pressure: 1 atm; temperature: 312 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCE73_sample_1isotropicE73_sample_conditions_1
3D HCCH-TOCSYE73_sample_1isotropicE73_sample_conditions_1
3D HBHA(CO)NHE73_sample_1isotropicE73_sample_conditions_1
2D 1H-13C HSQCE73_sample_1isotropicE73_sample_conditions_1
3D HNCAE73_sample_1isotropicE73_sample_conditions_1
3D H(CCO)NHE73_sample_1isotropicE73_sample_conditions_1
3D C(CO)NHE73_sample_1isotropicE73_sample_conditions_1
3D CBCA(CO)NHE73_sample_1isotropicE73_sample_conditions_1
3D HNCACBE73_sample_1isotropicE73_sample_conditions_1

Software:

TOPSPIN v2.1, Bruker Biospin - collection

NMRPipe v3.0, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

CSI v2.0, Wishart, D.S. and B.D. Sykes - Secondary Structure Information

SPARKY v3.115, T. D. Goddard and D. G. Kneller - chemical shift assignment, data analysis, peak picking

NMR spectrometers:

  • Bruker DRX 600 MHz

Related Database Links:

BMRB 17069 18132
PDB
GB AAR27912
REF NP_963940

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks