BMRB Entry 15891

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR15891

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Title:
1H, 15N chemical shifts of the UHM domain of Puf60 in complex with Prp16(201-238)
Deposition date:
2008-07-25
Original release date:
2008-10-15
Authors:
Corsini, Lorenzo; Sattler, Michael
Citation:

Citation: Corsini, Lorenzo; Sattler, Michael. "Backbone assignment of the UHM domain of Puf60 free and bound to five ligands"  Biomol. NMR Assignments 2, 211-214 (2008).
PubMed: 19636907

Assembly members:

Assembly members:
UHM_domain_of_Puf60, polymer, 110 residues, 12637.1 Da.
Prp16(201-238), polymer, 42 residues, 4619.8 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET9d

Entity Sequences (FASTA):

Entity Sequences (FASTA):
UHM_domain_of_Puf60: MKHHHHHHPMESTVMVLRNM VDPKDIDDDLEGEVTEECGK FGAVNRVIIYQEKQGEEEDA EIIVKIFVEFSIASETHKAI QALNGRWFAGRKVVAEVYDQ ERFDNSDLSA
Prp16(201-238): GAMGRHRPKDAATPSRSTWE EEDSGYGSSRRSQWESPSPT PS

Data sets:
Data typeCount
15N chemical shifts97
1H chemical shifts97

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1UHM domain of Puf601
2Prp16(201-238)2

Entities:

Entity 1, UHM domain of Puf60 110 residues - 12637.1 Da.

1   METLYSHISHISHISHISHISHISPROMET
2   GLUSERTHRVALMETVALLEUARGASNMET
3   VALASPPROLYSASPILEASPASPASPLEU
4   GLUGLYGLUVALTHRGLUGLUCYSGLYLYS
5   PHEGLYALAVALASNARGVALILEILETYR
6   GLNGLULYSGLNGLYGLUGLUGLUASPALA
7   GLUILEILEVALLYSILEPHEVALGLUPHE
8   SERILEALASERGLUTHRHISLYSALAILE
9   GLNALALEUASNGLYARGTRPPHEALAGLY
10   ARGLYSVALVALALAGLUVALTYRASPGLN
11   GLUARGPHEASPASNSERASPLEUSERALA

Entity 2, Prp16(201-238) 42 residues - 4619.8 Da.

1   GLYALAMETGLYARGHISARGPROLYSASP
2   ALAALATHRPROSERARGSERTHRTRPGLU
3   GLUGLUASPSERGLYTYRGLYSERSERARG
4   ARGSERGLNTRPGLUSERPROSERPROTHR
5   PROSER

Samples:

minimal_complex_of_Puf60_and_Prp16(201-238): UHM domain of Puf60, [U-100% 13C; U-100% 15N], 0.129 ± 0.01 mM; Prp16(201-238) 1.93 ± 0.01 mM; NaH2PO4 50 mM; NaCl 150 mM; b-mercaptoethanol 5 mM; H2O 95%; D2O, [U-100% 2H], 5%

sample_conditions_1: ionic strength: 0.2 M; pH: 6.8; pressure: 1 atm; temperature: 295 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCminimal_complex_of_Puf60_and_Prp16(201-238)isotropicsample_conditions_1

Software:

TOPSPIN v1.3, Bruker Biospin - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRView, Johnson, One Moon Scientific - chemical shift assignment

NMR spectrometers:

  • Bruker DRX 500 MHz

Related Database Links:

UNP Q9UHX1
BMRB 15885 15887 15888 15889 15890
PDB
DBJ BAE28998 BAE30474 BAE40622 BAF85062 BAI46053 BAA13213 BAF84323 BAG09667 BAG57191
EMBL CAG33424 CAH89379 CAH93447
GB AAB41656 AAD44358 AAF05605 AAF23589 AAF27522 AAC27431 AAC39729 AAH04235 AAH08340 AAI18086
REF NP_001039598 NP_001127017 NP_001128734 NP_001129505 NP_001158072 NP_001069266 NP_001099655 NP_001244810 NP_054722 XP_001500257
SP Q2HJG2 Q3UEB3 Q5R469 Q9UHX1 Q9WV25 Q17R09 Q92620
TPG DAA22863 DAA20084
AlphaFold Q9UJY7 Q2HJG2 Q3UEB3 Q5R469 Q9UHX1 Q9WV25 Q17R09 Q92620

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks