BMRB Entry 15890

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR15890

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Title:
1H, 15N chemical shifts of the UHM domain of Puf60 in complex with SF3b155(317-357)
Deposition date:
2008-07-25
Original release date:
2008-10-15
Authors:
Corsini, Lorenzo; Sattler, Michael
Citation:

Citation: Corsini, Lorenzo; Sattler, Michael. "Backbone assignment of the UHM domain of Puf60 free and bound to five ligands"  Biomol. NMR Assignments 2, 211-214 (2008).
PubMed: 19636907

Assembly members:

Assembly members:
UHM_domain_of_Puf60, polymer, 110 residues, 12637.1 Da.
SF3b155(317-357), polymer, 44 residues, 4414.8 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET9d

Entity Sequences (FASTA):

Entity Sequences (FASTA):
UHM_domain_of_Puf60: MKHHHHHHPMESTVMVLRNM VDPKDIDDDLEGEVTEECGK FGAVNRVIIYQEKQGEEEDA EIIVKIFVEFSIASETHKAI QALNGRWFAGRKVVAEVYDQ ERFDNSDLSA
SF3b155(317-357): GAMGRGGDSIGETPTPGASK RKSRWDETPASQMGGSTPVL TPGK

Data sets:
Data typeCount
15N chemical shifts94
1H chemical shifts94

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1UHM domain of Puf601
2SF3b155(317-357)2

Entities:

Entity 1, UHM domain of Puf60 110 residues - 12637.1 Da.

1   METLYSHISHISHISHISHISHISPROMET
2   GLUSERTHRVALMETVALLEUARGASNMET
3   VALASPPROLYSASPILEASPASPASPLEU
4   GLUGLYGLUVALTHRGLUGLUCYSGLYLYS
5   PHEGLYALAVALASNARGVALILEILETYR
6   GLNGLULYSGLNGLYGLUGLUGLUASPALA
7   GLUILEILEVALLYSILEPHEVALGLUPHE
8   SERILEALASERGLUTHRHISLYSALAILE
9   GLNALALEUASNGLYARGTRPPHEALAGLY
10   ARGLYSVALVALALAGLUVALTYRASPGLN
11   GLUARGPHEASPASNSERASPLEUSERALA

Entity 2, SF3b155(317-357) 44 residues - 4414.8 Da.

1   GLYALAMETGLYARGGLYGLYASPSERILE
2   GLYGLUTHRPROTHRPROGLYALASERLYS
3   ARGLYSSERARGTRPASPGLUTHRPROALA
4   SERGLNMETGLYGLYSERTHRPROVALLEU
5   THRPROGLYLYS

Samples:

minimal_complex_of_Puf60_and_SF3b155(317-357): UHM domain of Puf60, [U-100% 13C; U-100% 15N], 0.145 ± 0.01 mM; SF3b155(317-357) 0.227 ± 0.01 mM; NaH2PO4 50 mM; NaCl 150 mM; b-mercaptoethanol 5 mM; H2O 95%; D2O, [U-100% 2H], 5%

sample_conditions_1: ionic strength: 0.2 M; pH: 6.8; pressure: 1 atm; temperature: 295 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCminimal_complex_of_Puf60_and_SF3b155(317-357)isotropicsample_conditions_1

Software:

TOPSPIN v1.3, Bruker Biospin - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRView, Johnson, One Moon Scientific - chemical shift assignment

NMR spectrometers:

  • Bruker DRX 500 MHz

Related Database Links:

BMRB 15885 15887 15888 15889 15891
PDB
DBJ BAE28998 BAE30474 BAE40622 BAF85062 BAI46053 BAB28369 BAB40140 BAE24537 BAE27592
EMBL CAG33424 CAH89379 CAH93447 CAA70201
GB AAB41656 AAD44358 AAF05605 AAF23589 AAF27522 AAC97189 AAH03419 AAH15530 AAH28959 AAH29418
REF NP_001039598 NP_001127017 NP_001128734 NP_001129505 NP_001158072 NP_001084150 NP_001179923 NP_001252892 NP_036565 NP_112456
SP Q2HJG2 Q3UEB3 Q5R469 Q9UHX1 Q9WV25 O57683 O75533 Q99NB9
TPG DAA22863 DAA32571
AlphaFold Q9UHX1 Q5R469 Q3UEB3 Q2HJG2 Q9WV25 Q99NB9 O75533 O57683

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks