BMRB Entry 15888

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR15888

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Title:
1H, 15N chemical shifts of the UHM domain of Puf60 in complex with SF1(1-25)
Deposition date:
2008-07-25
Original release date:
2008-10-15
Authors:
Corsini, Lorenzo; Sattler, Michael
Citation:

Citation: Corsini, Lorenzo; Sattler, Michael. "Backbone assignment of the UHM domain of Puf60 free and bound to five ligands"  Biomol. NMR Assignments 2, 211-214 (2008).
PubMed: 19636907

Assembly members:

Assembly members:
UHM_domain_of_Puf60, polymer, 110 residues, 12637.1 Da.
SF1(1-25), polymer, 25 residues, 2876.2 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET9d

Entity Sequences (FASTA):

Entity Sequences (FASTA):
UHM_domain_of_Puf60: MKHHHHHHPMESTVMVLRNM VDPKDIDDDLEGEVTEECGK FGAVNRVIIYQEKQGEEEDA EIIVKIFVEFSIASETHKAI QALNGRWFAGRKVVAEVYDQ ERFDNSDLSA
SF1(1-25): MATGANATPLDFPSKKRKRS RWNQD

Data sets:
Data typeCount
15N chemical shifts92
1H chemical shifts92

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1UHM domain of Puf601
2SF1(1-25)2

Entities:

Entity 1, UHM domain of Puf60 110 residues - 12637.1 Da.

1   METLYSHISHISHISHISHISHISPROMET
2   GLUSERTHRVALMETVALLEUARGASNMET
3   VALASPPROLYSASPILEASPASPASPLEU
4   GLUGLYGLUVALTHRGLUGLUCYSGLYLYS
5   PHEGLYALAVALASNARGVALILEILETYR
6   GLNGLULYSGLNGLYGLUGLUGLUASPALA
7   GLUILEILEVALLYSILEPHEVALGLUPHE
8   SERILEALASERGLUTHRHISLYSALAILE
9   GLNALALEUASNGLYARGTRPPHEALAGLY
10   ARGLYSVALVALALAGLUVALTYRASPGLN
11   GLUARGPHEASPASNSERASPLEUSERALA

Entity 2, SF1(1-25) 25 residues - 2876.2 Da.

1   METALATHRGLYALAASNALATHRPROLEU
2   ASPPHEPROSERLYSLYSARGLYSARGSER
3   ARGTRPASNGLNASP

Samples:

minimal_complex_of_Puf60_and_SF1: UHM domain of Puf60, [U-100% 13C; U-100% 15N], 0.177 ± 0.01 mM; SF1(1-25) 0.333 ± 0.01 mM; NaH2PO4 50 mM; NaCl 150 mM; b-mercaptoethanol 5 mM; H2O 95%; D2O, [U-100% 2H], 5%

sample_conditions_1: ionic strength: 0.2 M; pH: 6.8; pressure: 1 atm; temperature: 295 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCminimal_complex_of_Puf60_and_SF1isotropicsample_conditions_1

Software:

TOPSPIN v1.3, Bruker Biospin - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRView, Johnson, One Moon Scientific - chemical shift assignment

NMR spectrometers:

  • Bruker DRX 500 MHz

Related Database Links:

BMRB 15885 15887 15889 15890 15891 18808
PDB
DBJ BAE28998 BAE30474 BAE40622 BAF85062 BAI46053 BAA05116 BAA05117 BAA05118 BAE01434 BAE26935
EMBL CAG33424 CAH89379 CAH93447 CAA03883 CAA59797 CAA70018 CAA70019 CAA73359
GB AAB41656 AAD44358 AAF05605 AAF23589 AAF27522 AAB03514 AAB04033 AAH08080 AAH08724 AAH09091
REF NP_001039598 NP_001127017 NP_001128734 NP_001129505 NP_001158072 NP_001075083 NP_001104261 NP_001104263 NP_001162562 NP_001164798
SP Q2HJG2 Q3UEB3 Q5R469 Q9UHX1 Q9WV25 Q15637 Q64213
TPG DAA22863 DAA13585
PIR S52735
AlphaFold Q2HJG2 Q3UEB3 Q5R469 Q9UHX1 Q9WV25 Q15637 Q64213

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks