BMRB Entry 15887

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR15887

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Title:
1H, 15N chemical shifts of the UHM domain of Puf60 in complex with U2AF65(85-112)
Deposition date:
2008-07-25
Original release date:
2008-10-15
Authors:
Corsini, Lorenzo; Sattler, Michael
Citation:

Citation: Corsini, Lorenzo; Sattler, Michael. "Backbone assignment of the UHM domain of Puf60 free and bound to five ligands"  Biomol. NMR Assignments 2, 211-214 (2008).
PubMed: 19636907

Assembly members:

Assembly members:
UHM_domain_of_Puf60, polymer, 110 residues, 12637.1 Da.
U2AF65(85-112), polymer, 36 residues, 4341.9 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET9d

Entity Sequences (FASTA):

Entity Sequences (FASTA):
UHM_domain_of_Puf60: MKHHHHHHPMESTVMVLRNM VDPKDIDDDLEGEVTEECGK FGAVNRVIIYQEKQGEEEDA EIIVKIFVEFSIASETHKAI QALNGRWFAGRKVVAEVYDQ ERFDNSDLSA
U2AF65(85-112): KKKVRKYWDVPPPGFEHITP MQYKAMQAGSHHHHHH

Data sets:
Data typeCount
15N chemical shifts84
1H chemical shifts84

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1UHM domain of Puf601
2U2AF65(85-112)2

Entities:

Entity 1, UHM domain of Puf60 110 residues - 12637.1 Da.

1   METLYSHISHISHISHISHISHISPROMET
2   GLUSERTHRVALMETVALLEUARGASNMET
3   VALASPPROLYSASPILEASPASPASPLEU
4   GLUGLYGLUVALTHRGLUGLUCYSGLYLYS
5   PHEGLYALAVALASNARGVALILEILETYR
6   GLNGLULYSGLNGLYGLUGLUGLUASPALA
7   GLUILEILEVALLYSILEPHEVALGLUPHE
8   SERILEALASERGLUTHRHISLYSALAILE
9   GLNALALEUASNGLYARGTRPPHEALAGLY
10   ARGLYSVALVALALAGLUVALTYRASPGLN
11   GLUARGPHEASPASNSERASPLEUSERALA

Entity 2, U2AF65(85-112) 36 residues - 4341.9 Da.

1   LYSLYSLYSVALARGLYSTYRTRPASPVAL
2   PROPROPROGLYPHEGLUHISILETHRPRO
3   METGLNTYRLYSALAMETGLNALAGLYSER
4   HISHISHISHISHISHIS

Samples:

minimal_complex_of_Puf60_and_U2AF65: UHM domain of Puf60, [U-100% 13C; U-100% 15N], 0.177 ± 0.01 mM; U2AF65(85-112) 0.333 ± 0.01 mM; NaH2PO4 50 mM; NaCl 150 mM; b-mercaptoethanol 5 mM; H2O 90%; D2O, [U-100% 2H], 10%

sample_conditions_1: ionic strength: 0.2 M; pH: 6.8; pressure: 1 atm; temperature: 295 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCminimal_complex_of_Puf60_and_U2AF65isotropicsample_conditions_1

Software:

TOPSPIN v1.3, Bruker Biospin - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRView, Johnson, One Moon Scientific - chemical shift assignment

NMR spectrometers:

  • Bruker DRX 500 MHz

Related Database Links:

UNP Q9UHX1 P26368
BMRB 15885 15888 15889 15890 15891
PDB
DBJ BAE28998 BAE30474 BAE40622 BAF85062 BAI46053
EMBL CAG33424 CAH89379 CAH93447
GB AAB41656 AAD44358 AAF05605 AAF23589 AAF27522 EHB13442
REF NP_001039598 NP_001127017 NP_001128734 NP_001129505 NP_001158072 XP_006216375
SP Q2HJG2 Q3UEB3 Q5R469 Q9UHX1 Q9WV25
TPG DAA22863
AlphaFold Q9UJY7 Q96HC5 Q2HJG2 Q3UEB3 Q5R469 Q9UHX1 Q9WV25

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks