BMRB Entry 17648

Title:
Partial 13C,15N chemical shift assignments of A30P alpha-synuclein fibrils
Deposition date:
2011-05-13
Original release date:
2011-07-07
Authors:
Comellas, Gemma; Lemkau, Luisel; Nieuwkoop, Andrew; Kloepper, Kathryn; Ladror, Daniel; Ebisu, Reika; Woods, Wendy; Andrew, Lipton; Julia, George; Rienstra, Chad
Citation:

Citation: Comellas, Gemma; Lemkau, Luisel; Nieuwkoop, Andrew; Kloepper, Kathryn; Ladror, Daniel; Ebisu, Reika; Woods, Wendy; Lipton, Andrew; George, Julia; Rienstra, Chad. "Structured Regions of -Synuclein Fibrils Include the Early-Onset Parkinson's Disease Mutation Sites."  J. Mol. Biol. 411, 881-895 (2011).
PubMed: 21718702

Assembly members:

Assembly members:
A30P_alpha-synuclein, polymer, 140 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: BL21 DE3 peT28

Data sets:
Data typeCount
13C chemical shifts44
15N chemical shifts14

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1A30P alpha-synuclein1

Entities:

Entity 1, A30P alpha-synuclein 140 residues - Formula weight is not available

1   METASPVALPHEMETLYSGLYLYSSERLYS
2   ALALYSGLUGLYVALVALALAALAALAGLU
3   LYSTHRLYSGLNGLYVALALAGLUALAPRO
4   GLYLYSTHRLYSGLUGLYVALLEUTYRVAL
5   GLYSERLYSTHRLYSGLUGLYVALVALHIS
6   GLYVALALATHRVALALAGLULYSTHRLYS
7   GLUGLNVALTHRASNVALGLYGLYALAVAL
8   VALTHRGLYVALTHRALAVALALAGLNLYS
9   THRVALGLUGLYALAGLYSERILEALAALA
10   ALATHRGLYPHEVALLYSLYSASPGLNLEU
11   GLYLYSASNGLUGLUGLYALAPROGLNGLU
12   GLYILELEUGLUASPMETPROVALASPPRO
13   ASPASNGLUALATYRGLUMETPROSERGLU
14   GLUGLYTYRGLNASPTYRGLUPROGLUALA

Samples:

sample_1: A30P alpha-synuclein, [U-13C; U-15N], mM; H2O 36%

sample_conditions_1: ionic strength: 0.05 M; pH: 7.5; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
NCACX 50 ms DARRsample_1isotropicsample_conditions_1
NCOCX 50 ms DARRsample_1isotropicsample_conditions_1
CANcoCX 50 ms DARRsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY, Goddard - data analysis

NMR spectrometers:

  • Varian UnityPlus 600 MHz

Related Database Links:

BMRB 16300 16302 16342 16543 16546 16547 16548 16904 16939 17214 17498 17649 17654 17665 17910 18207 18208 18243 18857 18860 19257 19337 19338 19344 19345 19350 19351 25227 25228
PDB
DBJ BAF82858 BAG73790
EMBL CAG33339 CAG46454
GB AAA16117 AAC02114 AAG30302 AAH13293 AAI08276
REF NP_000336 NP_001009158 NP_001129014 NP_001139526 NP_001139527
SP P37840 P61139 P61140 P61142 P61143
AlphaFold P61143 P61142 P61140 P61139 P37840