BMRB Entry 4809
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4809
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Citation: Hom, Kellie; Ma, Qi-Feng; Wolfe, Gary; Zhang, Hong; Storch, Elizabeth; Daggett, Valerie; Basus, Vladimir; Waskell, Lucy. "NMR Studies of the Association of Cytochrome b5 with Cytochrome c" Biochemistry ., .-..
Assembly members:
ferrous cytochrome b5, polymer, 82 residues, Formula weight is not available
cytochrome c, polymer, 104 residues, Formula weight is not available
HEM, non-polymer, 616.487 Da.
HEC, non-polymer, 618.503 Da.
Natural source: Common Name: bovine Taxonomy ID: 9913 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Bos taurus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pUC19
Entity Sequences (FASTA):
ferrous cytochrome b5: AVKYYTLEEIQKHNDSKSTW
LILHYKVYDLTKFLEEHPGG
EEVLREQAGGDATENFEDVG
HSTDARELSKTFIIGELHPD
DR
cytochrome c: GDVEKGKKIFVQKCAQCHTV
EKGGKHKTGPNLHGLFGRKT
GQAPGFTYTDANKNKGITWK
EETLMEYLENPKKYIPGTKM
IFAGIKKKTEREDLIAYLKK
ATNE
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 484 |
| 13C chemical shifts | 211 |
| 15N chemical shifts | 78 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | cytb5-N17D | 1 |
| 2 | cytc | 2 |
| 3 | heme | 3 |
| 4 | heme c | 4 |
Entities:
Entity 1, cytb5-N17D 82 residues - Formula weight is not available
| 1 | ALA | VAL | LYS | TYR | TYR | THR | LEU | GLU | GLU | ILE | ||||
| 2 | GLN | LYS | HIS | ASN | ASP | SER | LYS | SER | THR | TRP | ||||
| 3 | LEU | ILE | LEU | HIS | TYR | LYS | VAL | TYR | ASP | LEU | ||||
| 4 | THR | LYS | PHE | LEU | GLU | GLU | HIS | PRO | GLY | GLY | ||||
| 5 | GLU | GLU | VAL | LEU | ARG | GLU | GLN | ALA | GLY | GLY | ||||
| 6 | ASP | ALA | THR | GLU | ASN | PHE | GLU | ASP | VAL | GLY | ||||
| 7 | HIS | SER | THR | ASP | ALA | ARG | GLU | LEU | SER | LYS | ||||
| 8 | THR | PHE | ILE | ILE | GLY | GLU | LEU | HIS | PRO | ASP | ||||
| 9 | ASP | ARG |
Entity 2, cytc 104 residues - Formula weight is not available
| 1 | GLY | ASP | VAL | GLU | LYS | GLY | LYS | LYS | ILE | PHE | ||||
| 2 | VAL | GLN | LYS | CYS | ALA | GLN | CYS | HIS | THR | VAL | ||||
| 3 | GLU | LYS | GLY | GLY | LYS | HIS | LYS | THR | GLY | PRO | ||||
| 4 | ASN | LEU | HIS | GLY | LEU | PHE | GLY | ARG | LYS | THR | ||||
| 5 | GLY | GLN | ALA | PRO | GLY | PHE | THR | TYR | THR | ASP | ||||
| 6 | ALA | ASN | LYS | ASN | LYS | GLY | ILE | THR | TRP | LYS | ||||
| 7 | GLU | GLU | THR | LEU | MET | GLU | TYR | LEU | GLU | ASN | ||||
| 8 | PRO | LYS | LYS | TYR | ILE | PRO | GLY | THR | LYS | MET | ||||
| 9 | ILE | PHE | ALA | GLY | ILE | LYS | LYS | LYS | THR | GLU | ||||
| 10 | ARG | GLU | ASP | LEU | ILE | ALA | TYR | LEU | LYS | LYS | ||||
| 11 | ALA | THR | ASN | GLU |
Entity 3, heme - C34 H32 Fe N4 O4 - 616.487 Da.
| 1 | HEM |
Entity 4, heme c - C34 H34 Fe N4 O4 - 618.503 Da.
| 1 | HEC |
Related Database Links:
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks