BMRB Entry 17120

Title:
Binding of 1-methylimidazole to cytochrome c: kinetic analysis and resonance assignments by two-dimensional NMR
Deposition date:
2010-08-11
Original release date:
2010-11-10
Authors:
Shao, Weiping; Liu, Gaohua; Tang, Wenxia
Citation:

Citation: Shao, Weiping; Liu, Gaohua; Tang, Wenxia. "Binding of 1-methylimidazole to cytochrome c: kinetic analysis and resonance assignments by two-dimensional NMR"  Biochim. Biophys. Acta 1248, 177-185 (1995).
PubMed: 7748900

Assembly members:

Assembly members:
cytc, polymer, 105 residues, Formula weight is not available
1MZ, non-polymer, 83.112 Da.

Natural source:

Natural source:   Common Name: Horse   Taxonomy ID: 9795   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Equus caballus

Experimental source:

Experimental source:   Production method: obtained from a vendor

Data sets:
  • chemical rates
Data typeCount
binding constants5
kinetic rates4

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1cytc1
21MZ2

Entities:

Entity 1, cytc 105 residues - Formula weight is not available

1   METGLYASPVALGLULYSGLYLYSLYSILE
2   PHEVALGLNLYSCYSALAGLNCYSHISTHR
3   VALGLULYSGLYGLYLYSHISLYSTHRGLY
4   PROASNLEUHISGLYLEUPHEGLYARGLYS
5   THRGLYGLNALAPROGLYPHESERTYRTHR
6   ASPALAASNLYSASNLYSGLYILETHRTRP
7   LYSGLUGLUTHRLEUMETGLUTYRLEUGLU
8   ASNPROLYSLYSTYRILEPROGLYTHRLYS
9   METILEPHEALAGLYILELYSLYSLYSTHR
10   GLUARGGLUASPLEUILEALATYRLEULYS
11   LYSALATHRASNGLU

Entity 2, 1MZ - C4 H7 N2 - 83.112 Da.

1   1MZ

Samples:

sample_1: cytc 8 mM; 1MZ 40 mM; D2O 100%

sample_conditions_1: pH: 7.0; pressure: ambient atm; temperature: 319 K

Experiments:

NameSampleSample stateSample conditions
2D EXSYsample_1isotropicsample_conditions_1

Software:

software, na - na

NMR spectrometers:

  • Bruker AM 500 MHz

Related Database Links:

BMRB 1058 1107 1108 1109 1110 1111 1112 1113 1114 1116 1170 1171 1404 16759 17340 1736 1783 17847 17848 1785 1787 1789 216 220 224 2366 2367 2368 243 244 25640 274 285 286 316 317 336 4189 436 437 438 439
PDB
GB AAB33495 AAI05398 AAX77008 ABA06540 ABA06541
PRF 610169A 711086A
REF NP_001039526 NP_001123442 NP_001157486 XP_004007999 XP_004418964
SP P00004 P62894 P62895 P62896 P68096
TPG DAA30512
AlphaFold P62894 P00004 P62895 P62896 P68096