BMRB Entry 25070

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PDB ID: 2mre
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR25070
MolProbity Validation Chart

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Title:
NMR structure of the Rad18-UBZ/ubiquitin complex
Deposition date:
2014-07-03
Original release date:
2014-09-19
Authors:
Rizzo, Alessandro; Salerno, Paige; Bezsonova, Irina; Korzhnev, Dmitry
Citation:

Citation: Rizzo, Alessandro; Salerno, Paige; Bezsonova, Irina; Korzhnev, Dmitry. "NMR Structure of the Human Rad18 Zinc Finger in Complex with Ubiquitin Defines a Class of UBZ Domains in Proteins Linked to the DNA Damage Response."  Biochemistry 53, 5895-5906 (2014).
PubMed: 25162118

Assembly members:

Assembly members:
Ubiquitin, polymer, 79 residues, 8859.192 Da.
Ubiquitin-binding_zinc_finger_(UBZ)_domain_from_human_Rad18, polymer, 33 residues, 3628.145 Da.
ZINC ION, non-polymer, 65.409 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET15b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Ubiquitin: GSHMQIFVKTLTGKTITLEV EPSDTIENVKAKIQDKEGIP PDQQRLIFAGKQLEDGRTLS DYNIQKESTLHLVLRLRGG
Ubiquitin-binding_zinc_finger_(UBZ)_domain_from_human_Rad18: GSRQVTKVDCPVCGVNIPES HINKHLDSCLSRE

Data sets:
Data typeCount
1H chemical shifts732
13C chemical shifts438
15N chemical shifts106

Additional metadata:

  • Assembly
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Ubiquitin1
2Ubiquitin-binding_zinc_finger_(UBZ)_domain_from_human_Rad182
3ZINC ION3

Entities:

Entity 1, Ubiquitin 79 residues - 8859.192 Da.

the first three residues 'GSH' are remnants of the thrombin cleavage site and cloning vector

1   GLYSERHISMETGLNILEPHEVALLYSTHR
2   LEUTHRGLYLYSTHRILETHRLEUGLUVAL
3   GLUPROSERASPTHRILEGLUASNVALLYS
4   ALALYSILEGLNASPLYSGLUGLYILEPRO
5   PROASPGLNGLNARGLEUILEPHEALAGLY
6   LYSGLNLEUGLUASPGLYARGTHRLEUSER
7   ASPTYRASNILEGLNLYSGLUSERTHRLEU
8   HISLEUVALLEUARGLEUARGGLYGLY

Entity 2, Ubiquitin-binding_zinc_finger_(UBZ)_domain_from_human_Rad18 33 residues - 3628.145 Da.

GSR are remnants of the thrombin cleavage tag. Q198 is the first native residue

1   GLYSERARGGLNVALTHRLYSVALASPCYS
2   PROVALCYSGLYVALASNILEPROGLUSER
3   HISILEASNLYSHISLEUASPSERCYSLEU
4   SERARGGLU

Entity 3, ZINC ION - Zn - 65.409 Da.

1   ZN

Related Database Links:

BMRB 11505 11547 15047 15410 15689 15907 16228 16582 16626 16895 17181 17439 17769 17919 18582 18583 18584 18610 18611 18737 19406 19412 25123 25601 26604 4245 4375 25071
PDB 1AAR 1CMX 1D3Z 1F9J 1FXT 1G6J 1GJZ 1NBF 1OGW 1P3Q 1Q5W 1S1Q 1TBE 1UBI 1UBQ 1UZX 1V80 1V81 1VX7 1WR6 1WRD 1XD3 1XQQ 1YD8 1YIW 1YJ1 1YX5 1YX6 1ZGU 2AYO 2BGF 2C7M 2C7N 2D3G 2DEN 2DX5 2FCM 2FCN 2FCQ 2FCS 2FID 2FIF 2FUH 2G45 2GMI 2HD5 2HTH 2IBI 2J7Q 2JF5 2JRI 2JY6 2JZZ 2K25 2K39 2K6D 2K8B 2K8C 2KDE 2KDF 2KHW 2KJH 2KLG 2KN5 2KOX 2KTF 2KWU 2KWV 2KX0 2L0F 2L0T 2L3Z 2LD9 2LJ5 2LVO 2LVP 2LVQ 2LZ6 2MBB 2MBH 2MBO 2MBQ 2MCN 2MJ5 2MJB 2MOR 2MRE 2MRO 2MSG 2MUR 2MWS 2N2K 2NR2 2O6V 2OJR 2OOB 2PE9 2PEA 2QHO 2RR9 2RSU 2RU6 2W9N 2WDT 2WWZ 2WX0 2WX1 2XBB 2XEW 2XK5 2Y5B 2Z59 2ZCB 2ZCC 2ZNV 2ZVN 2ZVO 3A1Q 3A33 3A9J 3A9K 3AI5 3ALB 3AUL 3AXC 3B08 3B0A 3BY4 3C0R 3DVG 3DVN 3EEC 3EFU 3EHV 3H1U 3H7P 3H7S 3HM3 3I3T 3IFW 3IHP 3JSV 3JVZ 3JW0 3K9O 3K9P 3KVF 3KW5 3LDZ 3M3J 3MHS 3MTN 3N30 3N32 3NHE 3NOB 3NS8 3O65 3OFI 3OJ3 3OJ4 3ONS 3PHD 3PHW 3PRM 3PRP 3PT2 3PTF 3Q3F 3RUL 3TBL 3TMP 3U30 3UGB 3VDZ 3VFK 3VHT 3VUW 3VUX 3VUY 3WWQ 3WXE 3WXF 3WXG 3ZLZ 3ZNH 3ZNI 3ZNZ 4A18 4A19 4A1B 4A1D 4ADX 4AP4 4AUQ 4BBN 4BOS 4BOZ 4BVU 4CXC 4CXD 4D5L 4D61 4DDG 4DDI 4DHJ 4DHZ 4FJV 4HXD 4I6N 4IG7 4IUM 4JIO 4JQW 4K1R 4K7S 4K7U 4K7W 4KSK 4KSL 4KZX 4KZY 4KZZ 4LCD 4LDT 4LJO 4LJP 4M0W 4MDK 4MM3 4MSM 4MSQ 4NQK 4NQL 4P4H 4PIG 4PIH 4PIJ 4PQT 4R62 4RF0 4RF1 4S1Z 4S22 4UEL 4UF6 4UN2 4UPX 4UQ1 4UQ4 4UQ5 4V3K 4V3L 4W20 4W22 4W23 4W25 4W27 4W28 4WHV 4WLR 4WUR 4WZP 4XKL 4XOF 4XOK 4XOL 4XYZ 4Y1H 4Z9S 4ZFR 4ZFT 4ZPZ 5A5B 5AF4 5AF5 5AF6 5AIT 5AIU 5CAW 2MRF
DBJ BAA03983 BAA09860 BAA11842 BAA11843 BAA23486
EMBL CAA26488 CAA28495 CAA30183 CAA30815 CAA33466
GB AAA02769 AAA28154 AAA28997 AAA28998 AAA28999
PIR I50437 I51568 I65237 JN0790 S13928
PRF 0412265A 1212243A 1212243B 1212243C 1212243D
REF NP_001005123 NP_001006688 NP_001009117 NP_001009202 NP_001009286
SP P0C273 P0C275 P0C276 P0CG47 P0CG48
TPD FAA00319
TPE CEL68433 CEL70397 CEL75964 CEL78064
TPG DAA18802 DAA20663 DAA20672 DAA24675 DAA28295
AlphaFold P0C273 P0C275 P0C276 P0CG47 P0CG48

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