BMRB Entry 25071

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PDB ID: 2mrf
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR25071
MolProbity Validation Chart

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Title:
NMR structure of the ubiquitin-binding zinc finger (UBZ) domain from human Rad18
Deposition date:
2014-07-03
Original release date:
2014-09-19
Authors:
Rizzo, Alessandro; Salerno, Paige; Bezsonova, Irina; Korzhnev, Dmitry
Citation:

Citation: Rizzo, Alessandro; Salerno, Paige; Bezsonova, Irina; Korzhnev, Dmitry. "NMR Structure of the Human Rad18 Zinc Finger in Complex with Ubiquitin Defines a Class of UBZ Domains in Proteins Linked to the DNA Damage Response."  Biochemistry 53, 5895-5906 (2014).
PubMed: 25162118

Assembly members:

Assembly members:
Rad18-UBZ, polymer, 33 residues, 3628.145 Da.
ZINC ION, non-polymer, 65.409 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGEX

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Rad18-UBZ: GSRQVTKVDCPVCGVNIPES HINKHLDSCLSRE

Data sets:
Data typeCount
1H chemical shifts168
13C chemical shifts102
15N chemical shifts27

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1Rad18-UBZ1
2ZINC ION2

Entities:

Entity 1, Rad18-UBZ 33 residues - 3628.145 Da.

1   GLYSERARGGLNVALTHRLYSVALASPCYS
2   PROVALCYSGLYVALASNILEPROGLUSER
3   HISILEASNLYSHISLEUASPSERCYSLEU
4   SERARGGLU

Entity 2, ZINC ION - Zn - 65.409 Da.

1   ZN

Related Database Links:

PDB 2MRE 2MRF
REF XP_012616146

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