BMRB Entry 19789

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PDB ID: 2mkp
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR19789
MolProbity Validation Chart

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Title:
N domain of cardiac troponin C bound to the switch fragment of fast skeletal troponin I at pH 6
Deposition date:
2014-02-11
Original release date:
2014-02-11
Authors:
Robertson, Ian; Pineda-Sanabria, Sandra; Holmes, Peter; Sykes, Brian
Citation:

Citation: Robertson, Ian; Pineda-Sanabria, Sandra; Holmes, Peter; Sykes, Brian. "Conformation of the critical pH sensitive region of troponin depends upon a single residue in troponin I."  Arch. Biochem. Biophys. ., .-. (2013).
PubMed: 24333682

Assembly members:

Assembly members:
cNTnC, polymer, 89 residues, 10070.361 Da.
sTnI(115-131), polymer, 17 residues, 1862.297 Da.
CALCIUM ION, non-polymer, 40.078 Da.

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-3a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
cNTnC: MDDIYKAAVEQLTEEQKNEF KAAFDIFVLGAEDGCISTKE LGKVMRMLGQNPTPEELQEM IDEVDEDGSGTVDFDEFLVM MVRCMKDDS
sTnI(115-131): RMSADAMLKALLGSKHK

Data sets:
Data typeCount
13C chemical shifts94
15N chemical shifts82
1H chemical shifts416

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1cNTnC1
2sTnI(115-131)2
3entity_CA3

Entities:

Entity 1, cNTnC 89 residues - 10070.361 Da.

1   METASPASPILETYRLYSALAALAVALGLU
2   GLNLEUTHRGLUGLUGLNLYSASNGLUPHE
3   LYSALAALAPHEASPILEPHEVALLEUGLY
4   ALAGLUASPGLYCYSILESERTHRLYSGLU
5   LEUGLYLYSVALMETARGMETLEUGLYGLN
6   ASNPROTHRPROGLUGLULEUGLNGLUMET
7   ILEASPGLUVALASPGLUASPGLYSERGLY
8   THRVALASPPHEASPGLUPHELEUVALMET
9   METVALARGCYSMETLYSASPASPSER

Entity 2, sTnI(115-131) 17 residues - 1862.297 Da.

1   ARGMETSERALAASPALAMETLEULYSALA
2   LEULEUGLYSERLYSHISLYS

Entity 3, entity_CA - Ca - 40.078 Da.

1   CA

Related Database Links:

BMRB 15385 15388 15400 15427 16190 16752 17103 25034 25035 25120 25495 4401
PDB 1AJ4 1AP4 1DTL 1J1D 1J1E 1LA0 1LXF 1MXL 1SPY 1WRK 1WRL 2CTN 2JT0 2JT3 2JT8 2JTZ 2JXL 2KFX 2KGB 2KRD 2L1R 2MKP 2MZP 3SD6 3SWB 4GJE 4GJF
DBJ BAA02369 BAG36483
EMBL CAA30736 CAG46663 CAG46683 CAA32392 CAB59981 CAF89916 CAF89918 CAF89919
GB AAA36772 AAA37492 AAA37493 AAA48654 AAA49502 AAA19813 AAA31490 AAA40485 AAA42149 AAB26824
PIR S07450 TPHUCC
PRF 1403394A 1510257A 750650A 1914142A
REF NP_001029277 NP_001029523 NP_001123715 NP_001272501 NP_001291793 NP_001027530 NP_001076252 NP_001139301 NP_001139313 NP_001162377
SP P02591 P05936 P09860 P19123 P63315 P02643 P13412 P27768 P48788
TPG DAA16908 DAA13498
AlphaFold P02591 P05936 P09860 P19123 P63315 P02643 P13412 P27768 P48788

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