Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR16752
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Citation: Blumenschein, Tharin; Tripet, Brian; Hodges, Robert; Sykes, Brian. "Mapping the Interacting Regions between Troponins T and C" J. Biol. Chem. 276, 36606-36612 (2001).
PubMed: 11473120
Assembly members:
TnC, polymer, 161 residues, Formula weight is not available
TnT(160-193), polymer, 34 residues, Formula weight is not available
Natural source: Common Name: chicken Taxonomy ID: 9031 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Gallus gallus
Experimental source: Production method: chemical synthesis
Data type | Count |
binding constants | 4 |
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | TnC | 1 |
2 | TnT(160-193) | 2 |
Entity 1, TnC 161 residues - Formula weight is not available
1 | MET | ASP | ASP | ILE | TYR | LYS | ALA | ALA | VAL | GLU | ||||
2 | GLN | LEU | THR | GLU | GLU | GLN | LYS | ASN | GLU | PHE | ||||
3 | LYS | ALA | ALA | PHE | ASP | ILE | PHE | VAL | LEU | GLY | ||||
4 | ALA | GLU | ASP | GLY | CYS | ILE | SER | THR | LYS | GLU | ||||
5 | LEU | GLY | LYS | VAL | MET | ARG | MET | LEU | GLY | GLN | ||||
6 | ASN | PRO | THR | PRO | GLU | GLU | LEU | GLN | GLU | MET | ||||
7 | ILE | ASP | GLU | VAL | ASP | GLU | ASP | GLY | SER | GLY | ||||
8 | THR | VAL | ASP | PHE | ASP | GLU | PHE | LEU | VAL | MET | ||||
9 | MET | VAL | ARG | CYS | MET | LYS | ASP | ASP | SER | LYS | ||||
10 | GLY | LYS | THR | GLU | GLU | GLU | LEU | SER | ASP | LEU | ||||
11 | PHE | ARG | MET | PHE | ASP | LYS | ASN | ALA | ASP | GLY | ||||
12 | TYR | ILE | ASP | LEU | GLU | GLU | LEU | LYS | ILE | MET | ||||
13 | LEU | GLN | ALA | THR | GLY | GLU | THR | ILE | THR | GLU | ||||
14 | ASP | ASP | ILE | GLU | GLU | LEU | MET | LYS | ASP | GLY | ||||
15 | ASP | LYS | ASN | ASN | ASP | GLY | ARG | ILE | ASP | TYR | ||||
16 | ASP | GLU | PHE | LEU | GLU | PHE | MET | LYS | GLY | VAL | ||||
17 | GLU |
Entity 2, TnT(160-193) 34 residues - Formula weight is not available
1 | LEU | ALA | LYS | ALA | ASP | GLN | LYS | ARG | GLY | LYS | ||||
2 | LYS | GLN | THR | ALA | ARG | GLU | MET | LYS | LYS | LYS | ||||
3 | ILE | LEU | ALA | GLU | ARG | ARG | LYS | PRO | LEU | ASN | ||||
4 | ILE | ASP | HIS | LEU |
sample_1: TnC, [U-15N], 0.6 mM; TnT(160-193)21 62 mM; potassium chloride 100 mM; imidazole 10 mM; dithiothreitol 20 mM; CaCl2 6 mM; sodium azide 0.03%; 2,2-dimethyl-2-silapentane-5-sulfonic acid 0.2 mM; H2O 90%; D2O 10%
sample_2: TnC, [U-13C; U-15N], 0.9 mM; TnT(160-193) 33 mM; potassium chloride 100 mM; imidazole 10 mM; dithiothreitol 20 mM; CaCl2 6 mM; sodium azide 0.03%; 2,2-dimethyl-2-silapentane-5-sulfonic acid 0.2 mM; H2O 90%; D2O 10%
sample_conditions_1: pH: 6.9; pressure: 1 atm; temperature: 298 K
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
1D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
1D 1H-15N HSQC | sample_2 | isotropic | sample_conditions_1 |
2D 1H-15N HSQC | sample_2 | isotropic | sample_conditions_1 |
VNMR v6.1B, Varian - processing
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
NMRView, Johnson, One Moon Scientific - processing
BMRB | 16190 17103 19789 25034 25035 25120 25495 |
PDB | |
DBJ | BAA02369 BAG36483 BAB92975 BAB92976 BAB92977 BAB92978 BAB92979 |
EMBL | CAA30736 CAG46663 CAG46683 CAE45814 CAG46725 |
GB | AAA36772 AAA37492 AAA37493 AAA48654 AAA49502 AAA36777 AAA96440 AAA96441 AAA96442 AAA96443 |
PIR | S07450 TPHUCC TPRBTS |
PRF | 1403394A 1510257A 750650A 1810398A |
REF | NP_001029277 NP_001029523 NP_001123715 NP_001272501 NP_001291793 NP_001001441 NP_001001863 NP_001036245 NP_001036246 NP_001036247 |
SP | P02591 P05936 P09860 P19123 P63315 P02641 P09739 P45378 Q75NG9 Q8MKI3 |
TPG | DAA16908 DAA13525 |
AlphaFold | P02591 P05936 P09860 P19123 P63315 P02641 P09739 P45378 Q75NG9 Q8MKI3 |