BMRB Entry 18342

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PDB ID: 4asw
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR18342
MolProbity Validation Chart

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Title:
Backbone and sidechain assignments for Get5_UBL domain
Deposition date:
2012-03-21
Original release date:
2012-11-15
Authors:
Simon, Aline; Simpson, Peter; Isaacson, Rivka
Citation:

Citation: Simon, Aline; Simpson, Peter; Hawthorne, William; Hale, Lisa; Goldstone, Rachael; Isaacson, Rivka. "1H, 13C and 15N assignments of Sgt2N-terminal dimerisation domain and its binding partner, Get5 Ubiquitin-like domain."  Biomol. NMR Assignments 7, 271-274 (2013).
PubMed: 23001946

Assembly members:

Assembly members:
Get5_ubl, polymer, 97 residues, 10963.5 Da.

Natural source:

Natural source:   Common Name: Baker's yeast   Taxonomy ID: 4932   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET46

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Get5_ubl: AHHHHHHVDDDDKMDNAAVH LTLKKIQAPKFSIEHDFSPS DTILQIKQHLISEEKASHIS EIKLLLKGKVLHDNLFLSDL KVTPANSTITVMIKPNP

Data sets:
Data typeCount
13C chemical shifts383
15N chemical shifts82
1H chemical shifts601

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Get5_ubl1

Entities:

Entity 1, Get5_ubl 97 residues - 10963.5 Da.

1   ALAHISHISHISHISHISHISVALASPASP
2   ASPASPLYSMETASPASNALAALAVALHIS
3   LEUTHRLEULYSLYSILEGLNALAPROLYS
4   PHESERILEGLUHISASPPHESERPROSER
5   ASPTHRILELEUGLNILELYSGLNHISLEU
6   ILESERGLUGLULYSALASERHISILESER
7   GLUILELYSLEULEULEULYSGLYLYSVAL
8   LEUHISASPASNLEUPHELEUSERASPLEU
9   LYSVALTHRPROALAASNSERTHRILETHR
10   VALMETILELYSPROASNPRO

Related Database Links:

BMRB 18669 18671
PDB 2LXA 2LXC 3ZDM 4A20 4ASW 4GOC

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks