BMRB Entry 18669

Title:
Solution structure of the Get5 ubiquitin-like domain   PubMed: 23142665
Deposition date:
2012-08-19
Original release date:
2012-11-20
Authors:
Chartron, Justin; VanderVelde, David; Clemons, William
Citation:

Citation: Chartron, Justin; VanderVelde, David; Clemons, William. "Structures of the Sgt2/SGTA dimerization domain with the Get5/UBL4A UBL domain reveal an interaction that forms a conserved dynamic interface."  Cell Rep. 2, 1620-1632 (2012).

Assembly members:

Assembly members:
Get5, polymer, 87 residues, 9999.781 Da.

Natural source:

Natural source:   Common Name: Baker's yeast   Taxonomy ID: 4932   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Saccharomyces cerevisiae

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET33b

Experimental source:

Natural source:   Common Name: Baker's yeast   Taxonomy ID: 4932   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Saccharomyces cerevisiae

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET33b

Data sets:
Data typeCount
13C chemical shifts270
15N chemical shifts78
1H chemical shifts550

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Get51

Entities:

Entity 1, Get5 87 residues - 9999.781 Da.

1   METVALHISLEUTHRLEULYSLYSILEGLN
2   ALAPROLYSPHESERILEGLUHISASPPHE
3   SERPROSERASPTHRILELEUGLNILELYS
4   GLNHISLEUILESERGLUGLULYSALASER
5   HISILESERGLUILELYSLEULEULEULYS
6   GLYLYSVALLEUHISASPASNLEUPHELEU
7   SERASPLEULYSVALTHRPROALAASNSER
8   THRILETHRVALMETILELYSPROASNLEU
9   GLUHISHISHISHISHISHIS

Samples:

sample_1: Get5, [U-100% 13C; U-100% 15N], 1 mM; sodium phosphate 20 mM; sodium chloride 100 mM; sodium azide 0.02%; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 100 mM; pH: 6.1; pressure: 1 atm; temperature: 297.5 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D (H)CCH-TOCSYsample_1isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aliphaticsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1

Software:

VNMRJ, Varian - collection

Analysis, CCPN - chemical shift assignment

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

TALOS+, Cornilescu, Delaglio and Bax - data analysis

ARIA v2.3, Linge, O'Donoghue and Nilges - refinement, structure solution

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

BMRB 18342 18671
PDB
DBJ GAA26218
EMBL CAA88151 CAA99130 CAY86179
GB AHY77206 AJP41438 AJT70857 AJT71348 AJT71834
REF NP_014530
SP Q12285
TPG DAA10672
AlphaFold Q12285

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts