BMRB Entry 18669

Name: Solution structure of the Get5 ubiquitin-like domain
Published: 2012-11-20

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PDB ID: 2lxa
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR18669
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of the Get5 ubiquitin-like domain

Deposition date:

2012-08-19

Original release date:

2012-11-20

Authors:

Chartron, Justin; VanderVelde, David; Clemons, William

Citation:

Citation: Chartron, Justin; VanderVelde, David; Clemons, William. "Structures of the Sgt2/SGTA dimerization domain with the Get5/UBL4A UBL domain reveal an interaction that forms a conserved dynamic interface." Cell Rep. 2, 1620-1632 (2012).
PubMed: 23142665

Assembly members:

Assembly members:
Get5, polymer, 87 residues, 9999.781 Da.

Natural source:

Natural source: Common Name: Baker's yeast Taxonomy ID: 4932 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET33b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Get5: MVHLTLKKIQAPKFSIEHDF SPSDTILQIKQHLISEEKAS HISEIKLLLKGKVLHDNLFL SDLKVTPANSTITVMIKPNL EHHHHHH

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	270
15N chemical shifts	78
1H chemical shifts	550

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Get5	1

Entities:

Entity 1, Get5 87 residues - 9999.781 Da.

1

MET

VAL

HIS

LEU

THR

LEU

LYS

ILE

GLN

2

ALA

PRO

LYS

PHE

SER

ILE

GLU

HIS

ASP

PHE

3

SER

PRO

SER

ASP

THR

ILE

LEU

GLN

ILE

LYS

4

GLN

HIS

LEU

ILE

SER

GLU

LYS

ALA

SER

5

HIS

ILE

SER

GLU

ILE

LYS

LEU

LYS

6

GLY

LYS

VAL

LEU

HIS

ASP

ASN

LEU

PHE

LEU

7

SER

ASP

LEU

LYS

VAL

THR

PRO

ALA

ASN

SER

8

THR

ILE

THR

VAL

MET

ILE

LYS

PRO

ASN

LEU

9

GLU

HIS

Samples:

sample_1: Get5, [U-100% 13C; U-100% 15N], 1 mM; sodium phosphate 20 mM; sodium chloride 100 mM; sodium azide 0.02%; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 100 mM; pH: 6.1; pressure: 1 atm; temperature: 297.5 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D (H)CCH-TOCSY	sample_1	isotropic	sample_conditions_1
3D 1H-15N TOCSY	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC aliphatic	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1

Software:

VNMRJ, Varian - collection

Analysis, CCPN - chemical shift assignment

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

TALOS+, Cornilescu, Delaglio and Bax - data analysis

ARIA v2.3, Linge, O'Donoghue and Nilges - refinement, structure solution

NMR spectrometers:

Varian INOVA 600 MHz

BMRB	18342 18671
PDB	2LXA 2LXC 3ZDM 4A20 4ASW 4GOC
DBJ	GAA26218
EMBL	CAA88151 CAA99130 CAY86179
GB	AHY77206 AJP41438 AJT70857 AJT71348 AJT71834
REF	NP_014530
SP	Q12285
TPG	DAA10672
AlphaFold	Q12285