Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR16543
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Citation: Bodner, Christina; Maltsev, Alexander; Dobson, Christopher; Bax, Ad. "Differential phospholipid binding of alpha-synuclein variants implicated in Parkinson's disease revealed by solution NMR spectroscopy." Biochemistry 49, 862-871 (2010).
PubMed: 20041693
Assembly members:
monomer alpha-synuclein, polymer, 140 residues, 14470 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28(a)
Entity Sequences (FASTA):
monomer alpha-synuclein: MDVFMKGLSKAKEGVVAAAE
KTKQGVAEAAGKTKEGVLYV
GSKTKEGVVHGVATVAEKTK
EQVTNVGGAVVTGVTAVAQK
TVEGAGSIAAATGFVKKDQL
GKNEEGAPQEGILEDMPVDP
DNEAYEMPSEEGYQDYEPEA
Data type | Count |
15N chemical shifts | 133 |
1H chemical shifts | 133 |
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | monomer -synuclein | 1 |
Entity 1, monomer -synuclein 140 residues - 14470 Da.
1 | MET | ASP | VAL | PHE | MET | LYS | GLY | LEU | SER | LYS | |
2 | ALA | LYS | GLU | GLY | VAL | VAL | ALA | ALA | ALA | GLU | |
3 | LYS | THR | LYS | GLN | GLY | VAL | ALA | GLU | ALA | ALA | |
4 | GLY | LYS | THR | LYS | GLU | GLY | VAL | LEU | TYR | VAL | |
5 | GLY | SER | LYS | THR | LYS | GLU | GLY | VAL | VAL | HIS | |
6 | GLY | VAL | ALA | THR | VAL | ALA | GLU | LYS | THR | LYS | |
7 | GLU | GLN | VAL | THR | ASN | VAL | GLY | GLY | ALA | VAL | |
8 | VAL | THR | GLY | VAL | THR | ALA | VAL | ALA | GLN | LYS | |
9 | THR | VAL | GLU | GLY | ALA | GLY | SER | ILE | ALA | ALA | |
10 | ALA | THR | GLY | PHE | VAL | LYS | LYS | ASP | GLN | LEU | |
11 | GLY | LYS | ASN | GLU | GLU | GLY | ALA | PRO | GLN | GLU | |
12 | GLY | ILE | LEU | GLU | ASP | MET | PRO | VAL | ASP | PRO | |
13 | ASP | ASN | GLU | ALA | TYR | GLU | MET | PRO | SER | GLU | |
14 | GLU | GLY | TYR | GLN | ASP | TYR | GLU | PRO | GLU | ALA |
sample_1: sodium phosphate 20 mM; protein, [U-15N; U-2H], 0.6 mM; H2O 95%; D2O 5%
sample_conditions_1: pH: 6.0; pressure: 1 atm; temperature: 293 K
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - peak picking
BMRB | 16300 16302 16342 16546 16547 16548 16904 16939 17214 17498 17648 17649 17654 17665 17910 18207 18208 18243 18857 18860 19257 19337 19338 19344 19345 19350 19351 25227 25228 |
PDB | |
DBJ | BAB29375 BAF82858 BAG73790 |
EMBL | CAG33339 CAG46454 |
GB | AAA16117 AAC02114 AAG30302 AAH13293 AAI08276 |
REF | NP_000336 NP_001009158 NP_001032222 NP_001129014 NP_001139526 |
SP | P37840 P61139 P61140 P61142 P61143 |
AlphaFold | P37840 P61139 P61140 P61142 P61143 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks