BMRB Entry 15887

Name: 1H, 15N chemical shifts of the UHM domain of Puf60 in complex with U2AF65(85-112)
Published: 2008-10-15

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR15887

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 15N chemical shifts of the UHM domain of Puf60 in complex with U2AF65(85-112)

Deposition date:

2008-07-25

Original release date:

2008-10-15

Authors:

Corsini, Lorenzo; Sattler, Michael

Citation:

Citation: Corsini, Lorenzo; Sattler, Michael. "Backbone assignment of the UHM domain of Puf60 free and bound to five ligands" Biomol. NMR Assignments 2, 211-214 (2008).
PubMed: 19636907

Assembly members:

Assembly members:
UHM_domain_of_Puf60, polymer, 110 residues, 12637.1 Da.
U2AF65(85-112), polymer, 36 residues, 4341.9 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET9d

Entity Sequences (FASTA):

Entity Sequences (FASTA):
UHM_domain_of_Puf60: MKHHHHHHPMESTVMVLRNM VDPKDIDDDLEGEVTEECGK FGAVNRVIIYQEKQGEEEDA EIIVKIFVEFSIASETHKAI QALNGRWFAGRKVVAEVYDQ ERFDNSDLSA
U2AF65(85-112): KKKVRKYWDVPPPGFEHITP MQYKAMQAGSHHHHHH

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
15N chemical shifts	84
1H chemical shifts	84

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	UHM domain of Puf60	1
2	U2AF65(85-112)	2

Entities:

Entity 1, UHM domain of Puf60 110 residues - 12637.1 Da.

1	MET	LYS	HIS	HIS	HIS	HIS	HIS	HIS	PRO	MET
2	GLU	SER	THR	VAL	MET	VAL	LEU	ARG	ASN	MET
3	VAL	ASP	PRO	LYS	ASP	ILE	ASP	ASP	ASP	LEU
4	GLU	GLY	GLU	VAL	THR	GLU	GLU	CYS	GLY	LYS
5	PHE	GLY	ALA	VAL	ASN	ARG	VAL	ILE	ILE	TYR
6	GLN	GLU	LYS	GLN	GLY	GLU	GLU	GLU	ASP	ALA
7	GLU	ILE	ILE	VAL	LYS	ILE	PHE	VAL	GLU	PHE
8	SER	ILE	ALA	SER	GLU	THR	HIS	LYS	ALA	ILE
9	GLN	ALA	LEU	ASN	GLY	ARG	TRP	PHE	ALA	GLY
10	ARG	LYS	VAL	VAL	ALA	GLU	VAL	TYR	ASP	GLN
11	GLU	ARG	PHE	ASP	ASN	SER	ASP	LEU	SER	ALA

Entity 2, U2AF65(85-112) 36 residues - 4341.9 Da.

1

LYS

VAL

ARG

LYS

TYR

TRP

ASP

VAL

2

PRO

GLY

PHE

GLU

HIS

ILE

THR

PRO

3

MET

GLN

TYR

LYS

ALA

MET

GLN

ALA

GLY

SER

4

HIS

UNP	Q9UHX1 P26368
BMRB	15885 15888 15889 15890 15891
PDB	2DNY 3DXB 1JMT
DBJ	BAE28998 BAE30474 BAE40622 BAF85062 BAI46053
EMBL	CAG33424 CAH89379 CAH93447
GB	AAB41656 AAD44358 AAF05605 AAF23589 AAF27522 EHB13442
REF	NP_001039598 NP_001127017 NP_001128734 NP_001129505 NP_001158072 XP_006216375
SP	Q2HJG2 Q3UEB3 Q5R469 Q9UHX1 Q9WV25
TPG	DAA22863
AlphaFold	Q9UJY7 Q96HC5 Q2HJG2 Q3UEB3 Q5R469 Q9UHX1 Q9WV25

BMRB Entry 15887

Additional metadata:

Assembly:

Entities:

Samples:

Experiments:

Software:

NMR spectrometers:

Related Database Links:

1	MET	LYS	HIS	HIS	HIS	HIS	HIS	HIS	PRO	MET
2	GLU	SER	THR	VAL	MET	VAL	LEU	ARG	ASN	MET
3	VAL	ASP	PRO	LYS	ASP	ILE	ASP	ASP	ASP	LEU
4	GLU	GLY	GLU	VAL	THR	GLU	GLU	CYS	GLY	LYS
5	PHE	GLY	ALA	VAL	ASN	ARG	VAL	ILE	ILE	TYR
6	GLN	GLU	LYS	GLN	GLY	GLU	GLU	GLU	ASP	ALA
7	GLU	ILE	ILE	VAL	LYS	ILE	PHE	VAL	GLU	PHE
8	SER	ILE	ALA	SER	GLU	THR	HIS	LYS	ALA	ILE
9	GLN	ALA	LEU	ASN	GLY	ARG	TRP	PHE	ALA	GLY
10	ARG	LYS	VAL	VAL	ALA	GLU	VAL	TYR	ASP	GLN
11	GLU	ARG	PHE	ASP	ASN	SER	ASP	LEU	SER	ALA

1	MET	LYS	HIS	HIS	HIS	HIS	HIS	HIS	PRO	MET
2	GLU	SER	THR	VAL	MET	VAL	LEU	ARG	ASN	MET
3	VAL	ASP	PRO	LYS	ASP	ILE	ASP	ASP	ASP	LEU
4	GLU	GLY	GLU	VAL	THR	GLU	GLU	CYS	GLY	LYS
5	PHE	GLY	ALA	VAL	ASN	ARG	VAL	ILE	ILE	TYR
6	GLN	GLU	LYS	GLN	GLY	GLU	GLU	GLU	ASP	ALA
7	GLU	ILE	ILE	VAL	LYS	ILE	PHE	VAL	GLU	PHE
8	SER	ILE	ALA	SER	GLU	THR	HIS	LYS	ALA	ILE
9	GLN	ALA	LEU	ASN	GLY	ARG	TRP	PHE	ALA	GLY
10	ARG	LYS	VAL	VAL	ALA	GLU	VAL	TYR	ASP	GLN
11	GLU	ARG	PHE	ASP	ASN	SER	ASP	LEU	SER	ALA

1	MET	LYS	HIS	HIS	HIS	HIS	HIS	HIS	PRO	MET
2	GLU	SER	THR	VAL	MET	VAL	LEU	ARG	ASN	MET
3	VAL	ASP	PRO	LYS	ASP	ILE	ASP	ASP	ASP	LEU
4	GLU	GLY	GLU	VAL	THR	GLU	GLU	CYS	GLY	LYS
5	PHE	GLY	ALA	VAL	ASN	ARG	VAL	ILE	ILE	TYR
6	GLN	GLU	LYS	GLN	GLY	GLU	GLU	GLU	ASP	ALA
7	GLU	ILE	ILE	VAL	LYS	ILE	PHE	VAL	GLU	PHE
8	SER	ILE	ALA	SER	GLU	THR	HIS	LYS	ALA	ILE
9	GLN	ALA	LEU	ASN	GLY	ARG	TRP	PHE	ALA	GLY
10	ARG	LYS	VAL	VAL	ALA	GLU	VAL	TYR	ASP	GLN
11	GLU	ARG	PHE	ASP	ASN	SER	ASP	LEU	SER	ALA