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Sequence for Human DHFR:
1 VAL 2 GLY 3 SER 4 LEU 5 ASN
6 CYS 7 ILE 8 VAL 9 ALA 10 VAL
11 SER 12 GLN 13 ASN 14 MET 15 GLY
16 ILE 17 GLY 18 LYS 19 ASN 20 GLY
21 ASP 22 LEU 23 PRO 24 TRP 25 PRO
26 PRO 27 LEU 28 ARG 29 ASN 30 GLU
31 PHE 32 ARG 33 TYR 34 PHE 35 GLN
36 ARG 37 MET 38 THR 39 THR 40 THR
41 SER 42 SER 43 VAL 44 GLU 45 GLY
46 LYS 47 GLN 48 ASN 49 LEU 50 VAL
51 ILE 52 MET 53 GLY 54 LYS 55 LYS
56 THR 57 TRP 58 PHE 59 SER 60 ILE
61 PRO 62 GLU 63 LYS 64 ASN 65 ARG
66 PRO 67 LEU 68 LYS 69 GLY 70 ARG
71 ILE 72 ASN 73 LEU 74 VAL 75 LEU
76 SER 77 ARG 78 GLU 79 LEU 80 LYS
81 GLU 82 PRO 83 PRO 84 GLN 85 GLY
86 ALA 87 HIS 88 PHE 89 LEU 90 SER
91 ARG 92 SER 93 LEU 94 ASP 95 ASP
96 ALA 97 LEU 98 LYS 99 LEU 100 THR
101 GLU 102 GLN 103 PRO 104 GLU 105 LEU
106 ALA 107 ASN 108 LYS 109 VAL 110 ASP
111 MET 112 VAL 113 TRP 114 ILE 115 VAL
116 GLY 117 GLY 118 SER 119 SER 120 VAL
121 TYR 122 LYS 123 GLU 124 ALA 125 MET
126 ASN 127 HIS 128 PRO 129 GLY 130 HIS
131 LEU 132 LYS 133 LEU 134 PHE 135 VAL
136 THR 137 ARG 138 ILE 139 MET 140 GLN
141 ASP 142 PHE 143 GLU 144 SER 145 ASP
146 THR 147 PHE 148 PHE 149 PRO 150 GLU
151 ILE 152 ASP 153 LEU 154 GLU 155 LYS
156 TYR 157 LYS 158 LEU 159 LEU 160 PRO
161 GLU 162 TYR 163 PRO 164 GLY 165 VAL
166 LEU 167 SER 168 ASP 169 VAL 170 GLN
171 GLU 172 GLU 173 LYS 174 GLY 175 ILE
176 LYS 177 TYR 178 LYS 179 PHE 180 GLU
181 VAL 182 TYR 183 GLU 184 LYS 185 ASN
186 ASP
Formula Weight for Human DHFR: 21322 Da
Human VGSLNCIVAVSQNMGIGKNGDLPWPPLRNEFRYFQRMTTTSSVEGKQNLVIMGKKTWFSI 60
Chicken VRSLNSIVAVCQNMGIGKDGNLPWPPLRNEYKYFQRMTSTSHVEGKQNAVIMGKKTWFSI 60
Drosophila MLRFNLIVAVCENFGIGIRGDLPWR-IKSELKYFSRTTKRTSDPTKQNAVVMGRKTYFGV 59
E. coli --MISLIAALAVDRVIGMENAMPWN-LPADLAWFKRNTLN-------KPVIMGRHTWESI 50
:. *.*:. : ** . :** : : :*.* * : *:**::*: .:
Human PEKNRPLKGRINLVLSRELKEP--PQGAHFLSRSLDDALKLTEQPELANKVDMVWIVGGS 118
Chicken PEKNRPLKDRINIVLSRELKEA--PKGAHYLSKSLDDALALLDSPELKSKVDMVWIVGGT 118
Drosophila PESKRPLPDRLNIVLSTTLQESDLPKG-VLLCPNLETAMKILEE---QNEVENIWIVGGS 115
E. coli G---RPLPGRKNIILSSQPGTD----DRVTWVKSVDEAIAACGD------VPEIMVIGGG 97
*** .* *::** . .:: *: . * : ::**
Human SVYKEAMNHPGHLKLFVTRIMQDFESDTFFPEIDLEKYKLLPEYPGVLSDVQEEKGIKYK 178
Chicken AVYKAAMEKPINHRLFVTRILHEFESDTFFPEIDYKDFKLLTEYPGVPADIQEEDGIQYK 178
Drosophila GVYEEAMASPRCHRLYITKIMQKFDCDTFFPAIP-DSFREVAPDSDMPLGVQEENGIKFE 174
E. coli RVYEQFLPK--AQKLYLTHIDAEVEGDTHFPDYEPDDWESVFS---EFHDADAQNSHSYC 152
**: : :*::*:* ..: **.** ..:. : . : :.. .:
Human FEVYEKND--- 186
Chicken FEVYQKSVLAQ 189
Drosophila YKILEKHS--- 182
E. coli FEILERR---- 159
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