BMRB Entry 7432

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR7432

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Title:
Structural characterization of IscU and its interaction with HscB
Deposition date:
2008-09-28
Original release date:
2009-08-20
Authors:
Kim, Jin Hae; Fuzery, Anna; Marco, Tonelli; William, Westler; Markley, John
Citation:

Citation: Kim, Jin Hae; Fuzery, Anna; Tonelli, Marco; Ta, Dennis; Westler, William; Vickery, Larry; Markley, John. "Structure and dynamics of the iron-sulfur cluster assembly scaffold protein IscU and its interaction with the cochaperone HscB."  Biochemistry 48, 6062-6071 (2009).
PubMed: 19492851

Assembly members:

Assembly members:
IscU(D39A), polymer, 128 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pTrc99a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
IscU(D39A): MAYSEKVIDHYENPRNVGSF DNNDENVGSGMVGAPACGAV MKLQIKVNDEGIIEDARFKT YGCGSAIASSSLVTEWVKGK SLDEAQAIKNTDIAEELELP PVKIHCSILAEDAIKAAIAD YKSKREAK

Data sets:
Data typeCount
13C chemical shifts209
15N chemical shifts105
1H chemical shifts105
heteronuclear NOE values105
T1 relaxation values105
T2 relaxation values105

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1IscU(D39A)1

Entities:

Entity 1, IscU(D39A) 128 residues - Formula weight is not available

1   METALATYRSERGLULYSVALILEASPHIS
2   TYRGLUASNPROARGASNVALGLYSERPHE
3   ASPASNASNASPGLUASNVALGLYSERGLY
4   METVALGLYALAPROALACYSGLYALAVAL
5   METLYSLEUGLNILELYSVALASNASPGLU
6   GLYILEILEGLUASPALAARGPHELYSTHR
7   TYRGLYCYSGLYSERALAILEALASERSER
8   SERLEUVALTHRGLUTRPVALLYSGLYLYS
9   SERLEUASPGLUALAGLNALAILELYSASN
10   THRASPILEALAGLUGLULEUGLULEUPRO
11   PROVALLYSILEHISCYSSERILELEUALA
12   GLUASPALAILELYSALAALAILEALAASP
13   TYRLYSSERLYSARGGLUALALYS

Samples:

sample_1: IscU(D39A), [U-13C; U-15N], 2.5 mM; TRIS 20 mM; DTT 5-10 mM; EDTA 0.5 mM; sodium chloride 150 mM; DSS 0.7 mM; sodium azide 0.02%

sample_2: IscU(D39A), [U-15N], 0.6-0.7 mM; TRIS 20 mM; DTT 5-10 mM; EDTA 0.5 mM; sodium chloride 150 mM; DSS 0.7 mM; sodium azide 0.02%

sample_conditions_1: ionic strength: 0.15 M; pH: 8; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
2D 1H-15N NOESY HSQCsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_2isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - data analysis, processing

SPARKY, Goddard - chemical shift assignment, data analysis, peak picking

VNMRJ, Varian - collection

NMR spectrometers:

  • Varian Unity-Inova 600 MHz
  • Varian Unity-Inova 800 MHz

Related Database Links:

BMRB 15541 15967 16245 16603 17282 17836 17837 17844 18359 18360 18361 18362 18381 18750 18754
PDB
DBJ BAA16423 BAB36818 BAG78339 BAI26774 BAI31803
EMBL CAD02745 CAP76981 CAQ32902 CAQ88187 CAQ99420
GB AAC75582 AAG57643 AAL21436 AAN44075 AAN81505
PIR AE0824
REF NP_289086 NP_311422 NP_417024 NP_457073 NP_461477
SP P0ACD4 P0ACD5 P0ACD6 P0ACD7
AlphaFold P0ACD4 P0ACD5 P0ACD6 P0ACD7

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks