BMRB Entry 17844

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR17844

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Title:
The assigned chemical shifts of disordered-IscU complexed with IscS
Deposition date:
2011-08-09
Original release date:
2012-02-28
Authors:
Kim, Jin Hae; Tonelli, Marco; Markley, John
Citation:

Citation: Kim, Jin Hae; Tonelli, Marco; Markley, John. "Disordered form of the scaffold protein IscU is the substrate for iron-sulfur cluster assembly on cysteine desulfurase"  Proc. Natl. Acad. Sci. U. S. A. 109, 454-459 (2012).
PubMed: 22203963

Assembly members:

Assembly members:
IscU, polymer, 128 residues, Formula weight is not available
IscS, polymer, 404 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pTrc99a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
IscU: MAYSEKVIDHYENPRNVGSF DNNDENVGSGMVGAPACGDV MKLQIKVNDEGIIEDARFKT YGCGSAIASSSLVTEWVKGK SLDEAQAIKNTDIAEELELP PVKIHCSILAEDAIKAAIAD YKSKREAK
IscS: MKLPIYLDYSATTPVDPRVA EKMMQFMTMDGTFGNPASRS HRFGWQAEEAVDIARNQIAD LVGADPREIVFTSGATESDN LAIKGAANFYQKKGKHIITS KTEHKAVLDTCRQLEREGFE VTYLAPQRNGIIDLKELEAA MRDDTILVSIMHVNNEIGVV QDIAAIGEMCRARGIIYHVD ATQSVGKLPIDLSQLKVDLM SFSGHKIYGPKGIGALYVRR KPRVRIEAQMHGGGHERGMR SGTLPVHQIVGMGEAYRIAK EEMATEMERLRGLRNRLWNG IKDIEEVYLNGDLEHGAPNI LNVSFNYVEGESLIMALKDL AVSSGSACTSASLEPSYVLR ALGLNDELAHSSIRFSLGRF TTEEEIDYTIELVRKSIGRL RDLSPLWEMYKQGVDLNSIE WAHH

Data sets:
Data typeCount
13C chemical shifts77
15N chemical shifts77
1H chemical shifts77

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1IscU1
2IscS2

Entities:

Entity 1, IscU 128 residues - Formula weight is not available

1   METALATYRSERGLULYSVALILEASPHIS
2   TYRGLUASNPROARGASNVALGLYSERPHE
3   ASPASNASNASPGLUASNVALGLYSERGLY
4   METVALGLYALAPROALACYSGLYASPVAL
5   METLYSLEUGLNILELYSVALASNASPGLU
6   GLYILEILEGLUASPALAARGPHELYSTHR
7   TYRGLYCYSGLYSERALAILEALASERSER
8   SERLEUVALTHRGLUTRPVALLYSGLYLYS
9   SERLEUASPGLUALAGLNALAILELYSASN
10   THRASPILEALAGLUGLULEUGLULEUPRO
11   PROVALLYSILEHISCYSSERILELEUALA
12   GLUASPALAILELYSALAALAILEALAASP
13   TYRLYSSERLYSARGGLUALALYS

Entity 2, IscS 404 residues - Formula weight is not available

1   METLYSLEUPROILETYRLEUASPTYRSER
2   ALATHRTHRPROVALASPPROARGVALALA
3   GLULYSMETMETGLNPHEMETTHRMETASP
4   GLYTHRPHEGLYASNPROALASERARGSER
5   HISARGPHEGLYTRPGLNALAGLUGLUALA
6   VALASPILEALAARGASNGLNILEALAASP
7   LEUVALGLYALAASPPROARGGLUILEVAL
8   PHETHRSERGLYALATHRGLUSERASPASN
9   LEUALAILELYSGLYALAALAASNPHETYR
10   GLNLYSLYSGLYLYSHISILEILETHRSER
11   LYSTHRGLUHISLYSALAVALLEUASPTHR
12   CYSARGGLNLEUGLUARGGLUGLYPHEGLU
13   VALTHRTYRLEUALAPROGLNARGASNGLY
14   ILEILEASPLEULYSGLULEUGLUALAALA
15   METARGASPASPTHRILELEUVALSERILE
16   METHISVALASNASNGLUILEGLYVALVAL
17   GLNASPILEALAALAILEGLYGLUMETCYS
18   ARGALAARGGLYILEILETYRHISVALASP
19   ALATHRGLNSERVALGLYLYSLEUPROILE
20   ASPLEUSERGLNLEULYSVALASPLEUMET
21   SERPHESERGLYHISLYSILETYRGLYPRO
22   LYSGLYILEGLYALALEUTYRVALARGARG
23   LYSPROARGVALARGILEGLUALAGLNMET
24   HISGLYGLYGLYHISGLUARGGLYMETARG
25   SERGLYTHRLEUPROVALHISGLNILEVAL
26   GLYMETGLYGLUALATYRARGILEALALYS
27   GLUGLUMETALATHRGLUMETGLUARGLEU
28   ARGGLYLEUARGASNARGLEUTRPASNGLY
29   ILELYSASPILEGLUGLUVALTYRLEUASN
30   GLYASPLEUGLUHISGLYALAPROASNILE
31   LEUASNVALSERPHEASNTYRVALGLUGLY
32   GLUSERLEUILEMETALALEULYSASPLEU
33   ALAVALSERSERGLYSERALACYSTHRSER
34   ALASERLEUGLUPROSERTYRVALLEUARG
35   ALALEUGLYLEUASNASPGLULEUALAHIS
36   SERSERILEARGPHESERLEUGLYARGPHE
37   THRTHRGLUGLUGLUILEASPTYRTHRILE
38   GLULEUVALARGLYSSERILEGLYARGLEU
39   ARGASPLEUSERPROLEUTRPGLUMETTYR
40   LYSGLNGLYVALASPLEUASNSERILEGLU
41   TRPALAHISHIS

Samples:

IscU:IscS: IscU, [U-13C; U-15N], 1 mM; IscS 1.5 mM; TRIS 50 mM; DTT 5 mM; DSS 0.7 mM; EDTA 0.5 mM; H2O 93%; D2O 7%

sample_conditions_1: ionic strength: . M; pH: 7.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCIscU:IscSisotropicsample_conditions_1
3D HNCOIscU:IscSisotropicsample_conditions_1

Software:

VNMRJ, Varian - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY, Goddard - chemical shift assignment, data analysis, peak picking

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

BMRB 15967 16245 16603 17282 17836 17837 18359 18360 18361 18362 18381 18750 18754
PDB
DBJ BAA16423 BAB36818 BAG78339 BAH64655 BAI26774 BAA16424 BAB36819 BAG78340 BAI26775 BAI31804
EMBL CAD02745 CAP76981 CAQ32902 CAQ88187 CAQ99420 CAD02746 CAP76982 CAQ32903 CAQ88186 CAQ99421
GB AAC75582 AAG57643 AAL21436 AAN44075 AAN81505 AAG57644 AAL21437 AAN44076 AAN81506 AAO68037
PIR AE0824 A65030 AF0824 D91053 H85897
REF NP_311422 NP_417024 NP_457073 NP_461477 NP_708368 NP_289087 NP_311423 NP_457074 NP_461478 NP_708369
SP P0ACD4 P0ACD5 P0ACD6 P0ACD7 A7ZPX4 A8A336 A9MHJ4 A9N1X5 B1IWD1
AlphaFold P0ACD4 P0ACD5 P0ACD6 P0ACD7 A7ZPX4 A8A336 A9MHJ4 A9N1X5 B1IWD1

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks