BMRB Entry 15967
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR15967
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NMR-STAR v3 text file.
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Citation: Kim, Jin Hae; Fuzery, Anna; Tonelli, Marco; Ta, Dennis; Westler, William; Vickery, Larry; Markley, John. "Structure and Dynamics of the Iron-Sulfur Cluster Assembly Scaffold Protein IscU and Its Interaction with the Cochaperone HscB." Biochemistry 48, 6062-6071 (2009).
PubMed: 19492851
Assembly members:
IscU(D39A), polymer, 128 residues, Formula weight is not available
HscB, polymer, 171 residues, Formula weight is not available
Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Eubacteria Kingdom: not available Genus/species: Escherichia coli
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pTrc99a
Entity Sequences (FASTA):
IscU(D39A): MAYSEKVIDHYENPRNVGSF
DNNDENVGSGMVGAPACGAV
MKLQIKVNDEGIIEDARFKT
YGCGSAIASSSLVTEWVKGK
SLDEAQAIKNTDIAEELELP
PVKIHCSILAEDAIKAAIAD
YKSKREAK
HscB: MDYFTLFGLPARYQLDTQAL
SLRFQDLQRQYHPDKFASGS
QAEQLAAVQQSATINQAWQT
LRHPLMRAEYLLSLHGFDLA
SEQHTVRDTAFLMEQLELRE
ELDEIEQAKDEARLESFIKR
VKKMFDTRHQLMVEQLDNET
WDAAADTVRKLRFLDKLRSS
AEQLEEKLLDF
- assigned_chemical_shifts
| Data type | Count |
| 15N chemical shifts | 77 |
| 1H chemical shifts | 77 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | IscU(D39A) | 1 |
| 2 | HscB | 2 |
Entities:
Entity 1, IscU(D39A) 128 residues - Formula weight is not available
| 1 | MET | ALA | TYR | SER | GLU | LYS | VAL | ILE | ASP | HIS | ||||
| 2 | TYR | GLU | ASN | PRO | ARG | ASN | VAL | GLY | SER | PHE | ||||
| 3 | ASP | ASN | ASN | ASP | GLU | ASN | VAL | GLY | SER | GLY | ||||
| 4 | MET | VAL | GLY | ALA | PRO | ALA | CYS | GLY | ALA | VAL | ||||
| 5 | MET | LYS | LEU | GLN | ILE | LYS | VAL | ASN | ASP | GLU | ||||
| 6 | GLY | ILE | ILE | GLU | ASP | ALA | ARG | PHE | LYS | THR | ||||
| 7 | TYR | GLY | CYS | GLY | SER | ALA | ILE | ALA | SER | SER | ||||
| 8 | SER | LEU | VAL | THR | GLU | TRP | VAL | LYS | GLY | LYS | ||||
| 9 | SER | LEU | ASP | GLU | ALA | GLN | ALA | ILE | LYS | ASN | ||||
| 10 | THR | ASP | ILE | ALA | GLU | GLU | LEU | GLU | LEU | PRO | ||||
| 11 | PRO | VAL | LYS | ILE | HIS | CYS | SER | ILE | LEU | ALA | ||||
| 12 | GLU | ASP | ALA | ILE | LYS | ALA | ALA | ILE | ALA | ASP | ||||
| 13 | TYR | LYS | SER | LYS | ARG | GLU | ALA | LYS |
Entity 2, HscB 171 residues - Formula weight is not available
| 1 | MET | ASP | TYR | PHE | THR | LEU | PHE | GLY | LEU | PRO | ||||
| 2 | ALA | ARG | TYR | GLN | LEU | ASP | THR | GLN | ALA | LEU | ||||
| 3 | SER | LEU | ARG | PHE | GLN | ASP | LEU | GLN | ARG | GLN | ||||
| 4 | TYR | HIS | PRO | ASP | LYS | PHE | ALA | SER | GLY | SER | ||||
| 5 | GLN | ALA | GLU | GLN | LEU | ALA | ALA | VAL | GLN | GLN | ||||
| 6 | SER | ALA | THR | ILE | ASN | GLN | ALA | TRP | GLN | THR | ||||
| 7 | LEU | ARG | HIS | PRO | LEU | MET | ARG | ALA | GLU | TYR | ||||
| 8 | LEU | LEU | SER | LEU | HIS | GLY | PHE | ASP | LEU | ALA | ||||
| 9 | SER | GLU | GLN | HIS | THR | VAL | ARG | ASP | THR | ALA | ||||
| 10 | PHE | LEU | MET | GLU | GLN | LEU | GLU | LEU | ARG | GLU | ||||
| 11 | GLU | LEU | ASP | GLU | ILE | GLU | GLN | ALA | LYS | ASP | ||||
| 12 | GLU | ALA | ARG | LEU | GLU | SER | PHE | ILE | LYS | ARG | ||||
| 13 | VAL | LYS | LYS | MET | PHE | ASP | THR | ARG | HIS | GLN | ||||
| 14 | LEU | MET | VAL | GLU | GLN | LEU | ASP | ASN | GLU | THR | ||||
| 15 | TRP | ASP | ALA | ALA | ALA | ASP | THR | VAL | ARG | LYS | ||||
| 16 | LEU | ARG | PHE | LEU | ASP | LYS | LEU | ARG | SER | SER | ||||
| 17 | ALA | GLU | GLN | LEU | GLU | GLU | LYS | LEU | LEU | ASP | ||||
| 18 | PHE |
Samples:
sample_1: IscU(D39A), [U-15N], 0.7 mM; TRIS 20 mM; DTT 5-10 mM; EDTA 0.5 mM; sodium chloride 150 mM; DSS 0.7 mM; sodium azide 0.02%; HscB 1.4 mM
sample_conditions_1: ionic strength: 0.15 M; pH: 8; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N TROSY HSQC | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - data analysis, processing
SPARKY, Goddard - chemical shift assignment, data analysis, peak picking
VNMRJ, Varian - collection
NMR spectrometers:
- Varian Unity-Inova 600 MHz
Related Database Links:
| BMRB | 16245 16603 17282 17836 17837 17844 18359 18360 18361 18362 18381 18750 18754 15541 |
| PDB | |
| DBJ | BAA16423 BAB36818 BAG78339 BAI26774 BAI31803 BAA16421 BAB36816 BAG78337 BAI26772 BAI31801 |
| EMBL | CAD02745 CAP76981 CAQ32902 CAQ88187 CAQ99420 CAP76979 CAQ32900 CAQ88189 CAQ99418 CAR03969 |
| GB | AAC75582 AAG57643 AAL21436 AAN44075 AAN81505 AAA18299 AAC75580 AAG57641 AAN44073 AAN81502 |
| PIR | AE0824 E85897 |
| REF | NP_311422 NP_417024 NP_457073 NP_461477 NP_708368 NP_289084 NP_311420 NP_417022 NP_708366 NP_754934 |
| SP | P0ACD4 P0ACD5 P0ACD6 P0ACD7 A1AE64 A7ZPX0 A8A333 B1IWD4 B1LNI2 |
| AlphaFold | P0ACD4 P0ACD5 P0ACD6 P0ACD7 A1AE64 A7ZPX0 A8A333 B1IWD4 B1LNI2 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks