BMRB Entry 7325

Title:
Backbone and Ile(d1), Leu and Val Sidechain Resonance Assignments of the NUDIX Domain of Yeast Dcp2
Deposition date:
2006-11-04
Original release date:
2007-08-22
Authors:
Deshmukh, Mandar; Oku, Yuko; Gross, John
Citation:

Citation: Deshmukh, Mandar; Jones, Brittnee; Quang-Dang, Duc-Uy; Flinders, Jeremy; Floor, Stephen; Kim, Candice; Jemielity, Jacek; Kalek, Marcin; Darzynkiewicz, Edward; Gross, John. "mRNA decapping is promoted by an RNA-binding channel in Dcp2."  Mol. Cell 29, 324-336 (2008).
PubMed: 18280238

Assembly members:

Assembly members:
yDcp2(100-245), polymer, 146 residues, 17353.9 Da.

Natural source:

Natural source:   Common Name: Baker's Yeast   Taxonomy ID: 4932   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Saccharomyces Cerevisiae

Experimental source:

Experimental source:   Production method: recombinant technology   Vector: GB1-pET30a

Data sets:
Data typeCount
13C chemical shifts459
15N chemical shifts135
1H chemical shifts273

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1gb1-yDcp21

Entities:

Entity 1, gb1-yDcp2 146 residues - 17353.9 Da.

1   LYSLYSSERILEPROVALARGGLYALAALA
2   ILEPHEASNGLUASNLEUSERLYSILELEU
3   LEUVALGLNGLYTHRGLUSERASPSERTRP
4   SERPHEPROARGGLYLYSILESERLYSASP
5   GLUASNASPILEASPCYSCYSILEARGGLU
6   VALLYSGLUGLUILEGLYPHEASPLEUTHR
7   ASPTYRILEASPASPASNGLNPHEILEGLU
8   ARGASNILEGLNGLYLYSASNTYRLYSILE
9   PHELEUILESERGLYVALSERGLUVALPHE
10   ASNPHELYSPROGLNVALARGASNGLUILE
11   ASPLYSILEGLUTRPPHEASPPHELYSLYS
12   ILESERLYSTHRMETTYRLYSSERASNILE
13   LYSTYRTYRLEUILEASNSERMETMETARG
14   PROLEUSERMETTRPLEUARGHISGLNARG
15   GLNILELYSASNGLUASP

Samples:

sample_1: yDcp2(100-245), [U-2H; U-13C; U-15N], 0.5 mM; Na Phosphate 20 mM; Sodium Sulfate 100 mM; Arginine 50 mM; Glutamate 50 mM; NaCl 100 mM

sample_2: yDcp2(100-245), [U-2H; NA-Ile,Leu,Val metthyl; U-13C; U-15N], 0.5 mM; Na Phosphate 20 mM; Sodium Sulfate 100 mM; Arginine 50 mM; Glutamate 50 mM; NaCl 100 mM

NMR_Buffer: pH: 7.0; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
HNCAsample_1not availableNMR_Buffer
HN(CO)CAsample_1not availableNMR_Buffer
HN(CA)CBsample_1not availableNMR_Buffer
HN(COCA)CBsample_1not availableNMR_Buffer
HNCOsample_1not availableNMR_Buffer
HN(CA)COsample_1not availableNMR_Buffer
H(CCO)NHsample_1not availableNMR_Buffer
(H)C(CO)NHsample_1not availableNMR_Buffer

Software:

No software information available

NMR spectrometers:

  • Bruker Avance 500 MHz

Related Database Links:

PDB
DBJ GAA25980
EMBL CAA93389 CAA95998
GB AAA68866 AAC99860 ABN58539 ABN58548 ABN58557
REF NP_014281
SP A6ZRW5 P53550
TPG DAA10430
AlphaFold A6ZRW5 P53550

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks