BMRB Entry 7238

Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR7238

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Title:
NMR Investigation of Tyr105 Mutants in TEM-1 beta-lactamase Suggests Active-Site Dynamical Coupling
Deposition date:
2006-07-18
Original release date:
2007-01-09
Authors:
Doucet, Nicolas; Savard, Pierre-Yves; Pelletier, Joelle; Gagne, Stephane
Citation:

Citation: Savard, Pierre-Yves; Gagne, Stephane. "Backbone dynamics of TEM-1 determined by NMR: evidence for a highly ordered protein."  Biochemistry 45, 11414-11424 (2006).
PubMed: 16981701

Assembly members:

Assembly members:
TEM-1, polymer, 263 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
TEM-1: HPETLVKVKDAEDQLGARVG YIELDLNSGKILESFRPEER FPMMSTFKVLLCGAVLSRVD AGQEQLGRRIHYSQNDLVEN SPVTEKHLTDGMTVRELCSA AITMSDNTAANLLLTTIGGP KELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPAA MATTLRKLLTGELLTLASRQ QLIDWMEADKVAGPLLRSAL PAGWFIADKSGAGERGSRGI IAALGPDGKPSRIVVIYTTG SQATMDERNRQIAEIGASLI KHW

Data sets:
Data typeCount
13C chemical shifts248
15N chemical shifts248
1H chemical shifts248

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1TEM-1 Y105N1

Entities:

Entity 1, TEM-1 Y105N 263 residues - Formula weight is not available

1   HISPROGLUTHRLEUVALLYSVALLYSASP
2   ALAGLUASPGLNLEUGLYALAARGVALGLY
3   TYRILEGLULEUASPLEUASNSERGLYLYS
4   ILELEUGLUSERPHEARGPROGLUGLUARG
5   PHEPROMETMETSERTHRPHELYSVALLEU
6   LEUCYSGLYALAVALLEUSERARGVALASP
7   ALAGLYGLNGLUGLNLEUGLYARGARGILE
8   HISTYRSERGLNASNASPLEUVALGLUASN
9   SERPROVALTHRGLULYSHISLEUTHRASP
10   GLYMETTHRVALARGGLULEUCYSSERALA
11   ALAILETHRMETSERASPASNTHRALAALA
12   ASNLEULEULEUTHRTHRILEGLYGLYPRO
13   LYSGLULEUTHRALAPHELEUHISASNMET
14   GLYASPHISVALTHRARGLEUASPARGTRP
15   GLUPROGLULEUASNGLUALAILEPROASN
16   ASPGLUARGASPTHRTHRMETPROALAALA
17   METALATHRTHRLEUARGLYSLEULEUTHR
18   GLYGLULEULEUTHRLEUALASERARGGLN
19   GLNLEUILEASPTRPMETGLUALAASPLYS
20   VALALAGLYPROLEULEUARGSERALALEU
21   PROALAGLYTRPPHEILEALAASPLYSSER
22   GLYALAGLYGLUARGGLYSERARGGLYILE
23   ILEALAALALEUGLYPROASPGLYLYSPRO
24   SERARGILEVALVALILETYRTHRTHRGLY
25   SERGLNALATHRMETASPGLUARGASNARG
26   GLNILEALAGLUILEGLYALASERLEUILE
27   LYSHISTRP

Samples:

sample_1: TEM-1 (Y105N), [U-13C; U-15N], 0.8 mM; imidazole 4 mM; DSS 0.1 mM; H2O 90%; D2O 10%

conditions_1: pH: 6.6; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
15N-HSQCsample_1not availableconditions_1
3D-HNCOsample_1not availableconditions_1
3D-HN(CO)CAsample_1not availableconditions_1
3D-CBCA(CO)NHsample_1not availableconditions_1

Software:

VNMR v6.1c, Varian - spectra acquisition

NMRPipe v2.3, Frank Delaglio (Ad Bax) - Transform/process data

NMRView v5.2.2, One moon scientific - analyze data

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

BMRB 16392 6024 6357 7236 7237 7239
PDB
DBJ BAA00795 BAA03488 BAA12825 BAA14388 BAA19239
EMBL CAA04868 CAA05682 CAA05685 CAA05686 CAA05689
GB AAA24057 AAA25053 AAA32208 AAA53119 AAA53121
PIR S60310 S60312 T51301
PRF 2018199A
REF NP_052173 NP_569411 NP_608310 NP_758767 NP_775035
SP P62593 P62594 Q48406
TPE CAJ85677
AlphaFold P62593 P62594 Q48406

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks