BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 6357

Title: Backbone Resonance Assignments of Wild-Type TEM-1 Beta-lactamase from E. coli

Deposition date: 2004-10-14 Original release date: 2006-07-20

Authors: Julien, Olivier; Savard, Pierre-Yves; Gagne, Stephane

Citation: Julien, Olivier; Savard, Pierre-Yves; Gagne, Stephane. "Backbone Resonance Assignments of Wild-Type TEM-1 Beta-lactamase from E. coli"  The BMRB entry is the only known published source for the data. (2004).

Assembly members:
TEM-1 beta-lactamase, polymer, 263 residues, 28907 Da.

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pet24

Entity Sequences (FASTA):
TEM-1 beta-lactamase: HPETLVKVKDAEDQLGARVG YIELDLNSGKILESFRPEER FPMMSTFKVLLCGAVLSRVD AGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSA AITMSDNTAANLLLTTIGGP KELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPAA MATTLRKLLTGELLTLASRQ QLIDWMEADKVAGPLLRSAL PAGWFIADKSGAGERGSRGI IAALGPDGKPSRIVVIYTTG SQATMDERNRQIAEIGASLI KHW

Data sets:
Data typeCount
1H chemical shifts248
13C chemical shifts727
15N chemical shifts248

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1TEM-11

Entities:

Entity 1, TEM-1 263 residues - 28907 Da.

1   HISPROGLUTHRLEUVALLYSVALLYSASP
2   ALAGLUASPGLNLEUGLYALAARGVALGLY
3   TYRILEGLULEUASPLEUASNSERGLYLYS
4   ILELEUGLUSERPHEARGPROGLUGLUARG
5   PHEPROMETMETSERTHRPHELYSVALLEU
6   LEUCYSGLYALAVALLEUSERARGVALASP
7   ALAGLYGLNGLUGLNLEUGLYARGARGILE
8   HISTYRSERGLNASNASPLEUVALGLUTYR
9   SERPROVALTHRGLULYSHISLEUTHRASP
10   GLYMETTHRVALARGGLULEUCYSSERALA
11   ALAILETHRMETSERASPASNTHRALAALA
12   ASNLEULEULEUTHRTHRILEGLYGLYPRO
13   LYSGLULEUTHRALAPHELEUHISASNMET
14   GLYASPHISVALTHRARGLEUASPARGTRP
15   GLUPROGLULEUASNGLUALAILEPROASN
16   ASPGLUARGASPTHRTHRMETPROALAALA
17   METALATHRTHRLEUARGLYSLEULEUTHR
18   GLYGLULEULEUTHRLEUALASERARGGLN
19   GLNLEUILEASPTRPMETGLUALAASPLYS
20   VALALAGLYPROLEULEUARGSERALALEU
21   PROALAGLYTRPPHEILEALAASPLYSSER
22   GLYALAGLYGLUARGGLYSERARGGLYILE
23   ILEALAALALEUGLYPROASPGLYLYSPRO
24   SERARGILEVALVALILETYRTHRTHRGLY
25   SERGLNALATHRMETASPGLUARGASNARG
26   GLNILEALAGLUILEGLYALASERLEUILE
27   LYSHISTRP

Samples:

sample_1: TEM-1 beta-lactamase, [U-97.5% 13C; U-97.5% 15N], 1.0 mM; sodium phosphate 25 mM; Imidazole 4.0 mM; DSS 0.1 mM; sodium azide 0.1%; D2O 10%; H2O 90%

sample_2: TEM-1 beta-lactamase, [U-97.5% 15N], 1.0 mM; sodium phosphate 25 mM; Imidazole 4.0 mM; DSS 0.1 mM; sodium azide 0.1%; D2O 10%; H2O 90%

Ex-cond_1: pH: 6.6 na; temperature: 303 K; ionic strength: 0.05 M

Experiments:

NameSampleSample stateSample conditions
Chemical shifts were corrected for the TROSY experiment by adding 46.1Hz to thenot availablenot availableEx-cond_1
1H shifts and removing 46.1Hz to the 15N shifts.not availablenot availableEx-cond_1

Software:

Vnmr v6.1c - Acquisition of NMR experiments

NMRPipe v2.2 - Processing of NMR spectra

NMRView v5.2.2 - Sequential assignment

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

BMRB 16392 6024 7236 7237 7238 7239
PDB
DBJ BAA00795 BAA03488 BAA12825 BAA14388 BAA19239
EMBL CAA04868 CAA05682 CAA05685 CAA05686 CAA05689
GB AAA24057 AAA25053 AAA32208 AAA53119 AAA53121
PIR S60310 S60312 T51301
PRF 2018199A
REF NP_052173 NP_569411 NP_608310 NP_758767 NP_775035
SP P62593 P62594 Q48406
TPE CAJ85677

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts