BMRB Entry 6024

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6024
MolProbity Validation Chart

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Title:
1H, 13C and 15N backbone resonance assignment for TEM-1, a 28.9 kDa beta-lactamase from E. coli
Deposition date:
2003-11-27
Original release date:
2004-07-06
Authors:
Savard, Pierre-Yves; Levesque, Roger; Makinen, Marvin; Gagne, Stephane
Citation:

Citation: Savard, Pierre-Yves; Sosa-Peinado, Alejandro; Levesque, Roger; Makinen, Marvin; Gagne, Stephane. "Letter to the Editor: 1H, 13C and 15N backbone resonance assignments for TEM-1, a 28.9 kDa [bgr]-lactamase from E. coli"  J. Biomol. NMR 29, 433-434 (2004).
PubMed: 15213455

Assembly members:

Assembly members:
TEM-1 beta-lactamase, polymer, 263 residues, 28836 Da.

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pet24

Entity Sequences (FASTA):

Entity Sequences (FASTA):
TEM-1 beta-lactamase: HPGTLVKVKDAEDQLGARVG YIELDLNSGKILESFRPEER FPMMSTFKVLLCGAVLSRVD AGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSA AITMSDNTAANLLLTTIGGP KELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPAA MATTLRKLLTGELLTLASRQ QLIDWMEADKVAGPLLRSAL PAGWFIADKSGAGERGSRGI IAALGPDGKPSRIVVIYTTG SQATMDERNRQIAEIGASLI KHW

Data sets:
Data typeCount
13C chemical shifts759
15N chemical shifts249
1H chemical shifts249

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1TEM-11

Entities:

Entity 1, TEM-1 263 residues - 28836 Da.

1   HISPROGLYTHRLEUVALLYSVALLYSASP
2   ALAGLUASPGLNLEUGLYALAARGVALGLY
3   TYRILEGLULEUASPLEUASNSERGLYLYS
4   ILELEUGLUSERPHEARGPROGLUGLUARG
5   PHEPROMETMETSERTHRPHELYSVALLEU
6   LEUCYSGLYALAVALLEUSERARGVALASP
7   ALAGLYGLNGLUGLNLEUGLYARGARGILE
8   HISTYRSERGLNASNASPLEUVALGLUTYR
9   SERPROVALTHRGLULYSHISLEUTHRASP
10   GLYMETTHRVALARGGLULEUCYSSERALA
11   ALAILETHRMETSERASPASNTHRALAALA
12   ASNLEULEULEUTHRTHRILEGLYGLYPRO
13   LYSGLULEUTHRALAPHELEUHISASNMET
14   GLYASPHISVALTHRARGLEUASPARGTRP
15   GLUPROGLULEUASNGLUALAILEPROASN
16   ASPGLUARGASPTHRTHRMETPROALAALA
17   METALATHRTHRLEUARGLYSLEULEUTHR
18   GLYGLULEULEUTHRLEUALASERARGGLN
19   GLNLEUILEASPTRPMETGLUALAASPLYS
20   VALALAGLYPROLEULEUARGSERALALEU
21   PROALAGLYTRPPHEILEALAASPLYSSER
22   GLYALAGLYGLUARGGLYSERARGGLYILE
23   ILEALAALALEUGLYPROASPGLYLYSPRO
24   SERARGILEVALVALILETYRTHRTHRGLY
25   SERGLNALATHRMETASPGLUARGASNARG
26   GLNILEALAGLUILEGLYALASERLEUILE
27   LYSHISTRP

Samples:

sample_1: TEM-1 beta-lactamase, [U-97.5% 15N], 0.7 mM; sodium phosphate 25 mM; Imidazole 3.5 mM; DSS 0.1 mM; sodium azide 0.1%; D2O 10%; H2O 90%

sample_2: TEM-1 beta-lactamase, [U-97.5% 13C; U-97.5% 15N], 0.6 mM; sodium phosphate 25 mM; Imidazole 3.5 mM; DSS 0.1 mM; sodium azide 0.1%; D2O 10%; H2O 90%

sample_3: TEM-1 beta-lactamase, [U-97.5% 13C; U-97.5% 15N; U-96% 2H], 0.7 mM; sodium phosphate 25 mM; Imidazole 3.5 mM; DSS 0.1 mM; sodium azide 0.1%; D2O 10%; H2O 90%

Ex-cond_1: pH: 6.6 na; temperature: 303 K; ionic strength: 0.05 M

Experiments:

NameSampleSample stateSample conditions
Chemical shifts were corrected for the TROSY experiments by adding 46.1Hz tonot availablenot availableEx-cond_1
the 1H shifts and removing 46.1Hz to the 15N shiftsnot availablenot availableEx-cond_1
The 800MHz spectrometer used in this project is located at the NANUC (Alberta)not availablenot availableEx-cond_1

Software:

Vnmr v6.1c - Acquisition of NMR experiments

NMRPipe v2.2 - Processing of NMR spectra

NMRView v5.0.4 - Sequential assignment

NMR spectrometers:

  • Varian INOVA 600 MHz
  • Varian INOVA 800 MHz

Related Database Links:

BMRB 16392 6357 7236 7237 7238 7239
PDB
DBJ BAA00795 BAA03488 BAA12825 BAA14388 BAA19239
EMBL CAA04868 CAA05682 CAA05685 CAA05686 CAA05689
GB AAA24057 AAA25053 AAA32208 AAA53119 AAA53121
PIR S60310 S60312 T51301
PRF 2018199A
REF NP_052173 NP_569411 NP_608310 NP_758767 NP_775035
SP P62593 P62594 Q48406
TPE CAJ85677
AlphaFold P62593 P62594 Q48406 P62593 P62594 Q48406

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks