BMRB Entry 7208

Name: 1H, 13C, 15N Chemical shift assignment for Glycophorine A in bicelles and micelles
Published: 2007-01-09

Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR7208

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 13C, 15N Chemical shift assignment for Glycophorine A in bicelles and micelles

Deposition date:

2006-07-04

Original release date:

2007-01-09

Authors:

Mineev, Konstantin; Bocharov, Eduard; Maslennikov, Innokenty; Goncharuk, Marina; Arseniev, Alexander

Citation:

Citation: Mineev, Konstantin; Bocharov, Eduard; Maslennikov, Innokenty; Goncharuk, Marina; Arseniev, Alexander. "Structure and dynamics of glycophorin A TM segment in micelles and bicelles" The BMRB entry is the only known published source for the data..

Assembly members:

Assembly members:
GpATM, polymer, 38 residues, 4235 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
GpATM: RVQLAHHFSEPEITLIIFGV MAGVIGTILLISYGIRRL

Data sets:

assigned_chemical_shifts
- shifts_mic
- shifts_bic
coupling_constants
- J_coupling_list_mic
- J_coupling_list_bic
heteronucl_T1_relaxation
- T1_list_bic
- T1_list_mic
heteronucl_T2_relaxation
- T2_list_bic
- T2_list_mic

Data type	Count
13C chemical shifts	268
15N chemical shifts	71
1H chemical shifts	567
coupling constants	84
T1 relaxation values	64
T2 relaxation values	64

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	dimer of Glycophorin A transmembrane segments A	1
2	dimer of Glycophorin A transmembrane segments B	1

Entities:

Entity 1, dimer of Glycophorin A transmembrane segments A 38 residues - 4235 Da.

1

ARG

VAL

GLN

LEU

ALA

HIS

PHE

SER

GLU

2

PRO

GLU

ILE

THR

LEU

ILE

PHE

GLY

VAL

3

MET

ALA

GLY

VAL

ILE

GLY

THR

ILE

LEU

4

ILE

SER

TYR

GLY

ILE

ARG

LEU

Samples:

bic_hetero: GpATM, [U-15N; U-13C], 1.0 ± 0.2 mM; DMPC, [U-2H], 16.0 ± 1.0 mM; GpATM 1.0 ± 0.2 mM; DHPC, [U-2H], 64.0 ± 1.0 mM

mic_N15: GpATM, [U-15N], 2.0 ± 0.2 mM; Dodecylphosphocholine, [U-2H], 60.0 ± 2.0 mM

mic_N1513C: GpATM, [U-15N; U-13C], 2.0 ± 0.2 mM; Dodecylphosphocholine, [U-2H], 60.0 ± 2.0 mM

bic_15N13C: GpATM, [U-15N; U-13C], 2.0 ± 0.2 mM; DMPC, [U-2H], 16.0 ± 1.0 mM; DHPC, [U-2H], 64.0 ± 1.0 mM

bic_15N: GpATM, [U-15N], 2.0 ± 0.2 mM; DMPC, [U-2H], 16.0 ± 1.0 mM; DHPC, [U-2H], 64.0 ± 1.0 mM

mic_hetero: GpATM, [U-15N; U-13C], 1.0 ± 0.2 mM; Dodecylphosphocholine, [2H], 60.0 ± 2.0 mM; GpATM 1.0 ± 0.2 mM

micelles: pH: 6.0; temperature: 313 K

bicelles: pH: 5.4; temperature: 313 K

Experiments:

Name	Sample	Sample state	Sample conditions

Software:

VNMR -

NMR spectrometers:

Varian Unity 600 MHz

PDB	1AFO 2KPE 2KPF
DBJ	BAF83250
EMBL	CAA30843 CAA36095 CAC83868 CAC83869 CAC83870
GB	AAA18258 AAA52624 AAA52768 AAA88044 AAA88051
PRF	0412211A 1311302A 1314320A 1605205A
REF	NP_002090
SP	P02724
AlphaFold	P02724